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《Chinese Pharmacological Bulletin》 2013-08
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Effect of Aloe emodin on metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro

HE Zhen-hui1,HE Tai-ping2,WENG Shan-fan1,HUANG Yue-qun1,LIANG Nian-ci3(1.Dept of Laboratory Medicine,Medical College,Foshan University,Foshan Guangdong 528000,China; 2.Institute of biochemistry and molecular biology,Guangdong Medical College,Zhanjiang Guangdong 524023,China; 3.Guangdong Provincial Key Laboratory for the Research and Development of Natural Drugs,Zhanjiang Guangdong 524023,China)  
Aim To investigate the effect of Aloe emodin(AE) on metastasis-associated abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro.Methods MTT assay was used to evaluate the cell viability of MDA-MB-231 cells after 6h AE treatment.The effect of AE on the invasion and migration of MDA-MB-231 cells was determined by transwell chamber assay.RT-PCR,Western blot were applied to detect the expression levels of FAK mRNA and protein in MDA-MB-231 cells.The secretion of matrix metalloproteinase-9(MMP-9) from MDA-MB-231 cells was detected by zymography assay after AE treatment.Results AE significantly inhibited the invasion and migration of MDA-MB-231 cells in vitro,the inhibitory rates were(52.98±5.46)%,(45.88±8.51)% after 6h treatment with 80 μmol·L-1 AE.After 24h treatment,AE down-regulated the expression levels of FAK mRNA and protein of MDA-MB-231 cells.The secretion of MMP-9 from MDA-MB-231 cells was inhibited by 24h AE treatment.Conclusion AE can inhibit the invasion and migration of MDA-MB-231 cells.Its possible mechanism may be involved in the deduction of the expression level of FAK and the production of MMP-9.
【Fund】: 国家自然科学基金资助项目(No 39870900);; 佛山市医学类科技攻关项目(No 201108064);; 广东省中医药局建设中医药强省课题(No 20111057);; 佛山科学技术学院博士启动基金(No 2012006)
【CateGory Index】: R285.5
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【Citations】
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【Co-citations】
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