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《Chinese Pharmacological Bulletin》 2014-07
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Establishment of a rat model of trigeminal neuralgia induced by photochemical nerve injury

CUI Yue;ZHAO Jia;WANG Ye;SUN Dan-dan;ZHANG Ying;LIU Yang;ZHAO Xiao-liang;NIU Xiao-hong;ZHANG Mei-yu;WANG Dan-qiao;GAO Tian-le;XU Xiao-jun;Basic Medical School,Jilin University;Medical Experimental Center,China Academy of Chinese Medical Sciences;Karolinska Hospital-Huddinge;  
Aim To investigate the behavioral changes of the pain related neuromodulation and neurotransmission in peripheral and central nervous systems in rats with trigeminal neuralgia(TN) and provide a disease relevant animal model for mechanism study of TN. Methods The male SD rats were randomly divided into sham operation group and TN surgical group. The latter group was further divided into model group and gabapentin group(100 mg · kg- 1). TN was induced by intravenous erythrosine B injection and laser irradiation. The pain behavior of rats was evaluated using mechanical pain threshold measured with Von Frey hairs. Fluorescence quantitative PCR technique was deployed to study the change of Tac1 mRNA expressions in trigeminal ganglia. Utilizing microdialysis technique followed by high performance liquid chromatography fluorescence detection(HPLC-FLD),the extracellular striatum fluid was collected and glutamate(Glu) concentration was determined. Results In the model group,the average mechanical pain threshold in facial area innervated by the trigeminal nerve remained below 4g after 7days post surgery. The mechanical threshold of the model group(1.63 ±1. 27) g was significantly lower(P 0.01) than the control group(24. 17 ± 4. 49) g on day10 post surgery. In general,the mechanical withdraw threshold was decreased from the preoperative value of 26g to the postoperative value of(1. 60 ±1. 74) g(P 0. 01),and maintained stable at(0. 71 ± 1. 24)g during the whole dynamic monitoring period from day7 to day60. The successful rate of this model was 63%. After surgery,Tac1 mRNA expression in trigeminal ganglia and extracellular Glu levels in striatum were significantly up-regulated(P 0. 05) in the model group. Animals receiving Gabapentin showed significant improvement in pain symptoms,as well as reductions of Tac1 mRNA expression in trigeminal ganglia and extracellular Glu concentration in striatum(P 0. 05). Conclusions The above described photochemically induced TN rat model can partially mimic the clinical TN symptoms and its pathophysiology. Considering its overall high stability,it is very likely that this model could be used in preclinical mechanism study or drug screening of TN.
【Fund】: 国家科技部国际科技合作项目(No 2010DFA31890);; 中国中医科学院自主选题研究(No ZZ2013003)
【CateGory Index】: R745.11;R-332
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