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《Chinese Journal of Drug Evaluation》 2019-04
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Study on HPLC Fingerprint of Qinghouyan Mixture

JIANG Fan-cheng;LIANG Yun;YAN Zhang-yu;ZHAO Yue-ran;SUN Rui;LIU Ying;Dalian Inspection and Certification Technology Service Center;Dalian Food and Drug Safety Complaint Reporting and Acceptance Center;  
Objective: To establish a high performance liquid chromatographic fingerprint analysis method for Qinghouyan mixture,and provide evidence for its scientific identification and quality control. Methods: The column was ZORBAX SB-C_(18)( 4. 6 mm × 250 mm,5 μm). The mobile phase was gradient elution with acetonitrile-3 % acetic acid solution. The flow rate was 1. 0 mL·min~(-1).The detection wavelength was 270 nm. The column temperature was 30 ℃. Results: The chromatographic peaks of Qinghouyan mixture were well separated. The common pattern of fingerprints of Qinghouyan mixture was established. The distribution of 19 common fingerprint peaks,7 characteristic peaks and all chromatographic peaks were determined. Conclusion: The method is simple,reproducible and operability,and can be used to establish the quality standard of Qinghouyan mixture and the quality control of Qinghouyan mixture.
【CateGory Index】: R286.0
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