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《Clinical Medicine & Engineering》 2010-10
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Determinations of Active Compositions in Rhizoma Alismatis from Different Areas

WU Qiuyun1,CHEN Yanhong1,XIE Youliang2(1Pharmaceutical Department,Guangdong Provincial Hospital of TCM,Guangzhou 510120,China;2Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China)  
Objective To study the content of active compositions of Rhizoma Alismatis in different areas of the country in order to provide some academic basis for quality criterion of Rhizoma Alismatis. Methods Using the methods of HPLC to determine the content of Alisol A-24-acetate and Alisol B-23-acetate in Rhizoma Alismatis of different areas. This method was established on a Symmetry C18 column(200 mm × 4.6 mm 5 μm) with the mobile phase consisting of acetonitril-0.1% phosphonic acid(65: 35) and flow rate of 1.0 ml/min and the temperature at 25 ℃,the detection wavelength was 208 nm. Results The calibration curves showed good linearity in the range of 0.202 μg ~ 4.04 μg of Alisol A-24-acetate;0.406 μg ~ 8.120 μg of Alisol B-23-acetate,respectively. And the average recoveries for Alisol A-24-acetate and Alisol B-23-acetate were 97.8% and 98.3%,respectively. Conclusion The content of Alisol A-24-acetate and Alisol B-23-acetate are significantly different in Rhizoma Alismatis of different areas.
【CateGory Index】: R284
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