STUDIES ON PROLIFERATION INHIBITION AND ANTI - INVASION AND OF 10 - HYDROXYCAMPTOTHECIN IN HIGHLY METASTASIS HUMAN LUNG CANCER CELL LINE
Zhang Dongfang, Huang Wei, Huang Jiqun , et al. Cancer Institute, Guangzhou Medical College, Guangzhou 510182
Objective:To study the effects on proliferation inhibition and anti - invasion of 10 - hydroxycamp-tothecin (HCAM) in highly metastasis lung cancer cell line (PGCL3). Methods:The proliferation inhibitive rate, the colony- formation rate in soft agar, the invasive ability to reconstituted basement membrane , the laminin adhesion ability, the chemotatic migration ability, the activity of cathepsin B (CB) were used to observe the effect of HCAM on PGCL3 cells. Results: HCAM can decrease the proliferation ability of PGCL3 cells, and the ICso value are 0.17μmol/L. After treatment with 0.25μmol/L and 0.5 μmol/L HCAM for 96h, the invasive ability, the adhesion and migration ability, the secretion of CB and the colony - formation rate in semi - solid agar of the PGCL3 cells were significantly decreased ( P 0. 01), and the inhibition was in a dose -' dependent manner. Conclusion: HCAM can inhibit the proliferation and invasive ability of the PGCL3 human lung cancer cells. The mechanism of anti - invasion is not through blocking one aspect of the invasion but through inhibiting all the aspects of it.