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《Acta Genetica Sinica》 1997-05
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Genetic Divergence of Cordyceps sinensis as Estimated by Random Amplified Polymorphic DNA Analysis

CHEN Yongjiu~1 WANG Wen~1 YANG Yuexiong~2 SU Bing~1 ZHANG Yaping~1 XIONG Luyun~3 HE Zhaofan~3 SHU Chuang~2 YANG Darong~2 (1 Laboratory of Cellular and Molecular Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223) (2 Laboratory of Insect Resources Chinese Academy of Sciences Kunming 650223) (3 Institute of Biochem and Nutriology Guizhou Agricultural College Guiyang 550025)  
Random amplified polymorphic DNA(RAPD) has been used in analyzing 13 winterworm, summer grasses (Cordyceps sinensis [Berk.]. Sacc.) from 5 areas in Qingzangplateau. For 19 arbitrary primers, each individual showed about 65 RAPD markers.The genetic distances among those 13 winter worm, summer grasses were correlated totheir geographic distances. Molecular phylogenetic tree constructed by UPGMA and NJsuggested that 13 winter worm, summer grasses could be divided into 3 clusters: eastcluster, north cluster and middle cluster, in consistence with their origins and geneticdivergence among different clusters. Furthermore, our results implied that the RAPDmarkers of winter worm, summer grasses showed a geographic specificity and couldbe a better genetic marker in studying genetic diversity and phylogeny of winter worm,summer grasses.
【Fund】: 国家自然科学基金;; 云南省应用基础研究基金;; 国家杰出青年科学基金;; 云南省引进与培养人材基金
【CateGory Index】: S567.3
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