Studies on DNA Damage in Human Blood Lymphocytes Using the Single Cell Microgel Electrophoresis Technique
MENG Zi-qiang~1 ZHANG Lian-zhen~2(1 Division of Environmental Biological Toxicology Department of Life Sciences Shanxi University Taiyuan 030006) (2 China Institute of Radiation Protection Taiyuan 030006)
In this paper, the single cell microgel electrophoresis (SCG) technique wasdescribed in detail, and effects of DNA damage of human blood lymphocytes inducedby γ-rays radiation, H_2O_2 and CdCl_2 were studied using this technique. It was shownthat γ-rays, H_2O_2 and CdCl_2 all caused the increase of DNA migration length in thelymphocytes in the dose-dependent manner. The principle of the SCG techinque, causeof DNA migration in the untreated control cells, and noticeable details in the SCGtechnique were also discussed.