Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Genetica Sinica》 2002-12
Add to Favorite Get Latest Update

Studies of the Cellular Biological Function of Expression Change of RAB5A Gene in Human Lung Adenocarcinoma GLC-82 and SPC-al

LIU Fang Li ①, LI Yu, GAO Ling Han, FU Song Bin, LI Pu ( Laboratory of Medical Genetics,Harbin Medical University,Harbin 150086,China )  
To determine the effect of RAB5A gene over expression on invasion and differentiation of Human Lung Adnocarcinoma Cells SPC al and GLC 82. Using constructed antisense RNA of RAB5A (pcDNA3-AntiRAB5A) and RAB5A eukaryotic expression vector (pcDNA3.1-RAB5A),we stably transfected them into low differentiation human lung adenocarcinoma GLC 82 cell and human lung adenocarcinoma cells SPC al with low metastasis potential capability respectively in vitro . We observed the change of capability of transfected cells in invading recombinant basic membrane and chemotactic motion experiment in vitro , and we make use of hypodermic method with tumor cells in nude to observe the change of differentiation in transfected GLC 82 cells. The transfected GLC 82 cells with pcDNA3-antiRAB5A showed notable alteration. The capability of invading recombinant basic membrane and chemotatic motion decreased in transfected GLC 82 cells. The differentiation of transfected GLC 82 cells was apparently improved. The array of adenocytes is regular and it appears adenoid structure. After RAB5A eukaryotic expression plasmid was transfected into SPC al ,the invasive activity of cells is increased. Over expression of RAB5A played an important role in invasion and differentiation of human lung adenocarcinoma cells SPC al and GLC 82.
【Fund】: 国家自然科学基金 (3 9970 3 96) ;; 黑龙江省自然科学基金 (D980 9)资助~~
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Dongxing,YAN Dengguo,ZHAO Bingbo(Department of General Surgery,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China);Establishment of Subcutaneous Transplatation Tumor Models of Human Colon Carcinoma with Nude Mice[J];贵阳医学院学报;2010-03
2 ZHU Hua, XIAN Li-ping1, ZHANG Yu-meng1, LI Xiao-dong1, ZOU Deng-feng (Guilin Medical College, Guilin of 541004,P.R.China; Guangxi Traditional Chinese Medical University1, Nanning 530001,P.R.China);Human Cancer of the Stomach Transplanting Tumour Changes the Model of Nude Mouse Research General Situation[J];科学技术与工程;2007-23
3 ZHANG Xiao-ai,SHI Qing,WU Hua-cheng,CAO Wen-jun,SUN Shun-chang,NI Pei-hua,FAN Qi-shi(Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China);Suppression Effect of Introgeneous siRNA on Expression of RAB5A and RABEX5[J];上海交通大学学报(医学版);2006-02
【Citations】
Chinese Journal Full-text Database 5 Hits
1 YAN Cheng hui,WANG Bai qiu,WU Yan,FU Song bin,LI Pu (Laboratory of Medical Genetics,Harbin Medical University,Harbin,Heilongjiang,150086,China);Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines[J];遗传;2001-01
2 YAN Cheng-hui, WANG Bai-qiu , WU Yan, et al (Department of Medical Genetics, Harbin Medical University, Harbin 150086, China);Over-expression research of wild-type p53 gene in a pair of lung adenocarcinoma cell lines with different high-low metastasis potential: Anip973 and AGZY83-a[J];哈尔滨医科大学学报;2000-04
3 CHEN Yu 1,ZHANG Ze kun 2,ZOU Rong 1,et al (1.Medical Genetics Laboratory,Harbin Medical University;2.The Second Affiliated Hospital, Harbin Medical University,Harbin 150086,China);Construction of RAB5A gene eukaryotic expression vector by direct cloning[J];哈尔滨医科大学学报;2000-02
4 LI Yu, SONG Yan, LU Gang, ZOU Rong, ZOU Ya-nan, ZHANG Gui-yin, LI Pu (Laboratory of Medical Genetics, Harbin Medical Uniuersity, Harbin 150086, China);A Metastasis-Related Gene with Non-small Lung Carcinoma——RAB5A Gene[J];遗传;1999-04
5 LI Yu, FENG Huichen, CHEN Yü, et al. Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086;RAB5A, a gene possibly related to metastasis of human carcinoma of the lung and stomach[J];中华肿瘤杂志;1999-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 QU Yi-kun,XU Jian,ZHANG Ying-hai,LIU Wei-xin ( Department of General Surgery,the First Affiliated Hospital of Jiamusi Uni-versity,Jiamusi Heilongjiang 154002,China);Expression of RAB5A protein in human gastric carcinoma[J];局解手术学杂志;2013-04
2 GU Xinyue~1,CHA Nier~2,ZHANG Ming~3,LI Zhigao~1 1.Department of Colorectal Surgery,Affiliated Third Hospital of Harbin Medical University,Harbin 150081; 2.Department of Oncological Surgery,Inner Mongolia People's Hospital,Hohhot 010017;3.Department of Breast Surgery,Affiliated Third Hospital of Harbin Medical University,Harbin 150081.;The expression and correlation of Rab5A and CD44v9 in breast cancer tissues[J];实用肿瘤学杂志;2012-02
3 LI Xiao-dong,LIU Wen-sheng(The Fourth Hospital of Harbin Medical University,Harbin 150001,P.R.China);Expression of RAB5A Protein in Breast Caicinoma and Its Relation with Matastasis[J];科学技术与工程;2012-01
4 WANG Ying-jian,LI Hong-yuan,LI Yan,MIN Jie.Department of Endorcrine Surgery,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China;The Expression and Clinical Significance of RABEX-5 and RAB5 in Breast Cancer[J];四川大学学报(医学版);2011-02
5 JU Bang-l,CHEN Yong-bing,YU Cong-hui,et al(Department of Hepatobiliary Surgery,General Hospital of Chinese PLA Beijing Military Region,Beijing 100700,China);Clinical Significance of p53 protein expression in hepatolithiasis associated intrahepatic cholangiocarcinoma[J];中华保健医学杂志;2010-04
6 LI Zhi-gao,LI Xiao-dong,WANG Xi-yao,et al(Department of Abdominal Surgery,The Third Clinical College,Harbin Medical University,Harbin 150081,China);Inhibitory effect of RAB5A on the growth and metastasis of colorectal cancers in nude mice[J];哈尔滨医科大学学报;2007-03
7 LI Xiao-dong,LI Zhi-gao(Department of Surgery,The Third Clinical College,Harbin Medical University,Harbin 150040,China);The effect of RAB5A gene on the invasion of colorectal neoplasm cells[J];哈尔滨医科大学学报;2006-03
8 LI Xiao-dong(Department of Abdomen Surgery,The Affiliated Tumor Hospital of Harbin Medical University,Harbin 150040,China);Expression of RAB5A in Well or Poorly Differentiated Colorectal Cancers[J];黑龙江医学;2006-05
9 Zhi-Gao Li, Xiao-Dong Li, Xin-Shu Dong, Hong-Gang Pang, Jie Du, Department of Abdomen Surgery, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China;Significance of RAB5A and CD44v9 expression in colorectal cancer tissues[J];世界华人消化杂志;2006-09
10 ZHANG Xiao-ai,SHI Qing,WU Hua-cheng,CAO Wen-jun,SUN Shun-chang,NI Pei-hua,FAN Qi-shi(Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China);Suppression Effect of Introgeneous siRNA on Expression of RAB5A and RABEX5[J];上海交通大学学报(医学版);2006-02
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Bao-Lai, ZANG Kai-Hong, GAO Bei, LI Wen-Guang, GAO Ming-Tang, WU Yong -Jie School of Pharmacy, Lanzhou University, Lanzhou Medical College, Gansu Province Key Laboratory of Preclinical Study for New Traditional Chinese Medicine, Lanzh ou 730000, Gusan, China;Effects of different number of tumor cells on xenograft tumor growth and efficacy of anti-tumor agents in mice[J];中国临床药理学与治疗学;2004-12
2 RAO Lin,ZHAN Lin-sheng,PENG Jian-chun,WANG Quan-li* (Institute of Transfusion,Academy of Military Medical Sciences,Beijing 100850,China);Inhibition of Hepattis C Virus IRES Mediated Gene Expression by Short Interfering RNAs[J];中国生物化学与分子生物学报;2004-01
3 LIU Fang Li ①, LI Yu, GAO Ling Han, FU Song Bin, LI Pu ( Laboratory of Medical Genetics,Harbin Medical University,Harbin 150086,China );Studies of the Cellular Biological Function of Expression Change of RAB5A Gene in Human Lung Adenocarcinoma GLC-82 and SPC-al[J];遗传学报;2002-12
4 TAO Houquan *, YAO Ming, ZOU Shouchun, ZHAO Dajian, QIU Huasen. *Department of Surgery, Zhejiang Provincial People′s Hospital, Hangzhou 310014,China;Effect of angiogenesis inhibitor Rg3 on the growth and metastasis of gastric cancer in SCID mice[J];中华外科杂志;2002-08
5 LI Yu, SONG Yan, LU Gang, ZOU Rong, ZOU Ya-nan, ZHANG Gui-yin, LI Pu (Laboratory of Medical Genetics, Harbin Medical Uniuersity, Harbin 150086, China);A Metastasis-Related Gene with Non-small Lung Carcinoma——RAB5A Gene[J];遗传;1999-04
6 Wang Fangyu,Zhu Renmin,Wang L in etal Departmentof Gastroenterology,Jinling H ospital(Nanjing2 1 0 0 0 2 );Establishment of a model of human gastric cancer in nude mice via orthotopic transplantation[J];金陵医院学报;1997-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 TAO Wenhua;BU Ping;WANG Jijun;PAN Chenling;KONG Guimei;Department of Integrated Traditional Chinese and Western Medicine,Clinic College of Medicine of Yangzhou University;;Research progress of melittin in inhibiting the growth of gastrointestinal cancer[J];胃肠病学和肝病学杂志;2014-05
2 Yao Wenjian;Bai Yu;Zhao Baosheng;Department of Thoracic Surery,the First Affiliated Hospital of Xinxiang Medical College;Department of Pathology,Xinxiang Medical College;;Establishment of subcutaneous and orthotopic transplatation tumor model of human esophageal cancer in nude mice[J];重庆医学;2014-07
3 WANG Quan-kai;YANG Quan-hui;GUO Jing;XIE Guang-yun;CUI Tao;XU Rong-kun;XU Jian-ning;National Institute of Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention;Institute of Basic Medical Sciences Chinese Academy of Medical Sciences;;Establishment and application of implantation model of human hepatocellular carcinoma in nude mice[J];中草药;2014-03
4 Zhang Xiaohong;Jiang Hebi;Ye Tiezhen;Li Wenyi;Guangzhou Women and Children’s Medical Center;Department of Peadiatrics,The Second Affiliated Hospital,Sun Yat-sen University;;Establishment of the implanted animal model of human multidrug resistant cell line HL60/VCR in BALB/c-nu/nu mice[J];广州医药;2013-05
5 ZHEN Yun-huan1,YAN Deng-guo1,ZHANG Dong-xing2,LI Guo-sheng1,GONG Hui1.1.Department of Anus and Intestine Surgery,Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou Province,China;2.Department of General Surgery,The People's Hospital of Guizhou Province,Guiyang 550001,Guizhou Province,China;Sensitivity Test of Chemotherapy on Human Colorectal Cancer in Vivo[J];中国普外基础与临床杂志;2013-01
6 MA Mei1,2,ZHANG Yan2,ZHANG Ling1,WEI Qin1,DUAN Min-jun1,HOU Yue-mei1.(1.Xinjiang Key Laboratory for Medical Animal Model Research,First Teaching Hospital of Xinjiang Medical University, Urumqi 830054,China;2.College of Veterinary Medicine of Xinjiang Agricultural University,Urumqi 830052);Establishment of a nude mouse model of human gastric cancer and characterization of its biological features[J];中国实验动物学报;2012-06
7 CHENG Haiyu,YAN Dengguo,GONG Hui(Department of Anorectal Surgery,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China);Effects of Chemotherapy Drugs on the Expression of PCNA Protein in Transplantation Tumor of Colorectal Carcinoma in Nude Mouse[J];贵阳医学院学报;2012-06
8 XI Sheng-yan,Department of Traditional Chinese Medicine of Medical College of Xiamen University,Xiamen,Fujian 361005,China ZHANG Qian,XIE Hua,YUE Li-feng,GAO Xue-min,School of Basic Medical Sciences of Beijing University of Chinese Medicine,Beijing 100029,China LIU Chao-yang,Tumor research center,Cancer Hospital of Chinese Academy of Medical Sciences,Beijing 100021,China;Effects of hydroxy safflower Yellow-A on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice[J];Journal of Traditional Chinese Medicine;2012-02
9 ZHANG Yun-peng, SUN Peng~*.Department of General Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China;Establishment of gastric cancer model in nude mice[J];国际外科学杂志;2011-07
10 @ZHANG Yan$Xinjiang Laboratory Animal Center!Ulumuq 830002,China @FAN Wen-xi$Xinjiang HuaShiDan Pharmaceutical Research Co.LTD!Ulumuq 830000,China @XU Yi-mei$Xinjiang Laboratory Animal Center!Ulumuq 830002,China @SHI Sheng$Xinjiang Laboratory Animal Center!Ulumuq 830002,China @GUI You-jun$Xinjiang Laboratory Animal Center!Ulumuq 830002,China @YAN Shun-sheng$Xinjiang Laboratory Animal Center!Ulumuq 830002,China;Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice[J];实验动物与比较医学;2013-04
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Meng Xiangwen, Li Yu, Zhang Guiyin, et al. Department of Medical Genetics, Harbin Medical University, Harbin 150086;MOLECULAR CLONING OF TUMOR METASTASIS RELATED GENES FROM HUMAN LUNG ADENOCARCINOMA CELLS BY mRNA DIFFERENTIAL DISPLAY[J];中华医学遗传学杂志;1997-03
2 Wang Yuli et al Cell Culture Laboratory, Cancer Institute, Anshan Steel and Iron Co.;Establishment of Human Pulmonary Adenocarcinoma Cell Line and the Biologic Behavior[J];肿瘤临床;1985-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved