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《Acta Genetica Sinica》 2003-02
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A New Method for EST Clustering

ZHANG Li Da,YUAN De Jun,ZHANG Jian Wei,WANG Shi Ping ①,ZHANG Qi Fa (National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)  
We developed an EST (expressed sequence tag) clustering method,ESTClustering,to generate high quality unique expressed sequence based on large scale EST sequencing.The method uses consensus sequences to sequence analyze with megablast and assemble each cluster with phrap in clustering process.The clustering strategy can efficiently identify gene family and alternate splicing forms of expressed sequences.It can also reduce the adverse effects caused by sequence errors.The ESTClustering method tends to provide more expressed gene forms comparing with the UniGene clustering method of the National Center for Biotechnology Information.Analysis of the 112 256 ESTs of Arabidopsis with ESTClustering produced 23 581 EST clusters.Among these Arabidopsis EST clusters,13 597 have corresponding genome coding sequences and this number is close to the number of genes predicted with Arabidopsis ESTs.Using this clustering method,a total of 147 191 rice ESTs were clustered into 33 896 groups.
【Fund】: 国家重点基础发展规划 (973 )资助项目~~
【CateGory Index】: Q75
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Chinese Journal Full-text Database 3 Hits
1 MO Hui\|dong (Lab of Quantitative genetics, Yangzhou Univ, Yangzhou, 225009, China);LOOK BACK AND REFLECT ON GENETIC RESEARCHES OF VARIATION FOR QUANTITATIVE TRAITS A CHALLENGE FOR QUANTITATIVE GENETICS IN POST-GENOME ERA[J];Journal of Yandzhou University;2003-02
2 YI Le-fei 1,LIU Chu-wu 2,WANG Ping 1,ZHOU Xiang-hong 1 (1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China);cDNA cloning and characterization of a novel ubiquitin-conjugating enzyme gene from Porphyra yezoensis Ueda[J];Journal of Fisheries of China;2009-05
3 YI Le-fei1,WANG Ping1,ZHOU Xiang-hong1,LIU Chu-wu2 (1. Jiangsu Key Laboratory of Marine Biotechnology,HuaiHai Institute of Technology,Lianyungang 222005,China;2. Fisheries College, Guangdong Ocean University,Zhanjiang 524088,China);In silico cloning and analysis of cysteine synthase gene from Porphyra yezoensis Ueda[J];Journal of Fishery Sciences of China;2009-04
Chinese Journal Full-text Database 10 Hits
1 LI En-min, XU Li-yan, YANG Fan, et al(Biochemistry and Molecular Biology Department, Shantou University Medical College, Shantou 515031, China);SCREENING DIFFERENTIALLY EXPRESSED GENES FROM SUPPRESSION SUBTRACTIVE HYBRIDIZATIONAL POSITIVE CLONES USING REVERSE mRNA DOT BLOT TECHNIQUE[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-02
2 Jiang Changjun (Depp. Tment of Tea Science, Anhui Agricultural University, Hefei 230036);The construction of cDNA library of pollen from Brassica nupus[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1995-03
3 GAO Jian~1, XU Xiao-feng~1 , WU Hui-ling~1, CHEN Xue ping~2, ZHOU Jia-hao~3(1. College of Life Science, Nanjing Normal University, Nanjing 210097; 2. College of Economics and Technology,University of Science and Technology of China,Hefei 230052; 3.Zhong-ao Environment Protection Co.Itd,Xuancheng 242000);Construction of Full-length cDNA Library from A NativeTobacco Germplasm and Its Quality Analysis[J];Journal of Anhui Agricultural University;2004-01
4 GUAN Yan ying1,YAN fei2,YANG Xiao lou (1.Department of Biology,Baoding Teachers College,Baoding 071051; 2 College of Life Science,Hebei University,Baoding 071002,China;3.Anxin County Junior High school,Anxin 071600,China);Process of Study on Transformation of the Medical Plants by Agrobacterium Rhizogenes[J];Journal of Baoding Teachers College;2003-02
5 Gao Da wei (YanShan University,Material College, Qinhuangdao Hebei 066004,China);Applications of Random Amplified Polymorphic DNA to Analyze and Show Genetic Diversity[J];JOURNAL OF YELLOW CATTLE SCIENCE;2000-03
6 CHANG Zhen Zhan 1, GUO De An 1, ZHENG Jun Hua 1 JIN Ming Shan 2, Xie Mei Yin 2, SHEN Xin 3, WANG Sha Sheng 3 (1.School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083, China; 2. Beijing First School of Light In;Hairy root culture and anthraquinone production of Cassia obtusifolia L in a bioreactor[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;2000-02
7 ZHANG Li rong, XU Da qing, LIU Da qun (College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China);SSR marker, ISSR marker and their application to plant genetics and breeding[J];Journal of Agricultural University of Hebei;2002-01
8 GUI Xiu-mei,ZHANG Jin-lin,DONG Jin-gao,AN Xin-long(Lab of Mycotoxin,Agricultural University of Hebei, Baoding 071001, China);Establishment of the optimal RAPD reaction system of Setosphaeria turcica[J];Journal of Agricultural University of Hebei;2005-01
9 ;Washington, D.C.[J];Joy English;2005-11
10 CHEN Dafu 1, 3 NIU Baolong 2 WENG Hongbiao 2 MENG Zhiqi 2 L Shunlin 1 ( 1 College of Animal Science, Zhejiang University, Hangzhou 310029; 2 Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021; 3 College of Bee Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002);Cloning of Adenine Nucleotide Translocase Gene From Bombyx mori Based on EST Database[J];Acta Sericologica Sinica;2004-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YI Le-fei et al(School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Cloning and Analysis of BADH Gene from Helianthus annuus[J];Journal of Anhui Agricultural Sciences;2008-32
2 WANG Wei et al (College of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Quantitative Traits Loci Mapping Methods of Diploid Traits[J];Journal of Anhui Agricultural Sciences;2009-13
3 ZHANG Wen-guang~1, WANG Ya-chun~2, LI Jin-quan~1(1.College of Animal Science and Veterinary Medicine, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia 010018, China; 2. College of Animal Science and Technology, China Agricultural University, Beijing 010018, China);Quantitative Genetics, Genomics and the Future of Animal Breeding[J];Journal Yellow Cattle Science;2004-05
4 ZHOU Xiao-guo~(1,2),ZHANG Zheng-bin~1 (1 The Research Center of Agricultural Resources,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China;2 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);Advances in Loci Mapping and Analysis of Quantitative Traits of Crops[J];Acta Botanica Boreali-occidentalia Sinica;2005-03
5 DU Chun-fang 1,2,LI Peng-bo1,LI Run-zhi 2* (1 Cotton Research Institute,Shanxi Academy of Agricultural Sciences,Yuncheng,Shanxi 044000,China;2 Center for Agricultural Biotechnology,Shanxi Agricultural University,Taigu,Shanxi 030801,China);Research Advances in Molecular Basis of the Variations and QTL Cloning of Quantitative Plant Traits[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
6 CHEN Yong-zhong, WANG Xiang-nan(Hunan Forestry Academy, Changsha 410004, China);The outlook of the biotechnology breeding research on oil tea Camellia[J];Hunan Forestry Science and Technology;2005-04
7 ZHANG Ri-Qing,DING Zhi-Lei,ZHANG Xu,WEN Li(College of Resource and Environment,Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Literature Review of Genetic Improvement in Oiltea[J];Nonwood Forest Research;2006-04
8 HE Xiao-hong, HU Zhi-qiu, WANG Wei, XU Chen-wu (Dept of Agron, Nanjing Agric Univ, Nanjing, 210095, China) Lab of Quant Gen, Yangzhou Univ, Yangzhou, 225009, China);Statistical method for mapping QTL controlling endosperm traits in cereals[J];Journal of Yandzhou University;2005-01
9 AO Yan, XU Chen-wu (Lab of Quant Gen, Yangzhou Univ, Yangzhou, 225009, China);Bayesian method of linear regression analysis and its application in QTL mapping[J];Journal of Yandzhou University;2005-02
10 PAN Xue-biao, ZHANG Ya-fang, ZUO Shi-min, CHEN Zong-xiang ou, 225009, China);Discussion on QTLs identification and application for important quantitative traits in crops[J];Journal of Yandzhou University;2005-02
China Proceedings of conference Full-text Database 1 Hits
1 SONG Xiu-Fang;SONG Tong-Ming;DAI Jing-Rui;LI Jian-Sheng National Maize Improvement Center,China Agricultural University,Beijing, 100094;QTL mapping for kernel traits in high-oil maize[A];[C];2004
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