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Identification of the Triploid Hybrid Chromosomes of Elymus canadensis L.×Hordeum brivisubulatum

Link.by Genomic in situ Hybridization YU Zhuo~(1,①),YUN Jin-Feng~1,MA You-Zhi~2,XIN Zhi-Yong~2 (1.Agronomy College of Inner Mongolia Agricultural University,Hohhot 010019,China; 2.Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Science,Beijing 100081,China)  
The intergeneric F 1 hybrid between Elymus canadensis L.and Hordeum brivisubulatum Link.is a triploid (2n=3x=21),in which 7 chromosomes,that is equal to the base number of parent's chromosomes,were lost.In order to indentify the chromosome constitution of the triploid F 1 hybrid of E.canadensis L.×H.brivisubulatum Link.,the genomic in situ hybridization of F 1 root tip cell chromosome DNA that H.brivisubulatum H 1H 1H 2H 2 genome DNA labeled with Biotin-16-dUTP was conducted using E.canadensis SSH CH C genome as blocking DNA.The result showed that the chromosomes of triploid F 1 hybrid consisted of 7 chromosomes from E.canadensis S genome and 14 chromosomes from H.brivisubulatum H 1H 2 genome while the 7 chromosomes of H C genome of E.canadensis were lost.
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