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Improvement Of Plant Regeneration Frequency in vitro In Indica Rice

Tian Wenzhong(Institute of Genetics Academia Sinica Beijing 100101)Iann Rance;Elunialai Sivamani;Claude Fauquet;Roger N.Beachy(International Laboratory for Tropical Agricultural Biotechnology The Scripps Research Institute La Jolla CA 92037 USA)  
In order to improve the frequency of plant regeneration from calli in indica rice,the influences of different factors on plant regeneration were investigated.Supplement with cytokinins(KT,BAP,Zeatin or 2ip,mg/l)and NAA(1 mg/l),or supplement with thidiazuron(TDZ,5 mg/l)in the induction medium or subculture medium,and partial desiccation of callus before transfer to regeneration medium have been found significantly increase the frequency of plant regeneration in indica rice.5-14 folds more plants were obtained than untreated control by the combination of these treatments with indica varieties TN1,IR72 and IR64.
【CateGory Index】: S511.210.3
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