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《ACTA GENETICA SINICA》 1995-01
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Structural Analysis of the Gene Coding for Cellobiobydrase II of Trichoderma viride

Wang Jianrong(Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences, Shanghai 200030)Zhang Manfu(College of Biology, Beijing Agricultural University Beijing 100094)Huang Tao(Institute of Developmental Biology, Acadm  
Restriction analysis and bi-directional southern blotting were conducted to localize the CBHII gene of Trichoderma viride in 4kb SolI insert cloned in plasmid pUC19.A 4.4kb EcoRI fragment containing T. viride CBHII gene was verified and subcloned into plasmid pUC19. The physical map of the 4.4kb EcoRI fragment in recombinant pCBHⅡ was then established. Based on the map 18 restriction fragments were subcloned into appropriate plasmids. A11 the short fragments were sequenced by the method of Sanger′s dideoxy chain termination,and 4074bp of DNA sequence was determined which revealed that the CBHII gene of T. viride had a length of 1613bp,flanked by 744bp at 5′end and 1718bp at 3'end. It was deduced that the CBH II gene was composed of 4 exons and 3 introns. The cis-regulatory elements,similar to those in most eukaryotic genes, were found upstream of the CBHII structural gene,but the downstream region of the gene lacked of the sequences associated with the function of transcriptional termination and polyadenylation of mRNA in most eukaryotes, whereas,this region had short repeated sequences and pyrimidine-rich sequences with unknown function. The homology between cellulase genes and their possible function were also discussed.
【CateGory Index】: Q343
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