Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Genetic Analysis of Transgenes in the Progenies of Bt Rice Crossed to Conventional Rice Varieties

WANG Zhong-hua,CUI Hai-rui,SHU Qing-yao,XIANG You-bing,WU Dian-xing,GAO Ming wei,XIAYing-wu (Institute of Nuclear Agricultural Sciences,Zhejiang University, Huajiachi Hangzhou,310029,China) Xiong ying Cheng, Illimar Altosaar (Department of Biochemi  
Improved histochemical staining for GUS activity,PCR and Western blotting were used to detect the population of Bt rice crossed to conventional rice varieties A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants.The result demonstrated that cry1Ab gene closely inherited and expressed with reporter gene gus.Therefore,it is possible to develop GUS assisted selection to preliminarily identify the Bt gene and study the inheritance of transgenes in (back)cross breeding.Mendelian segragation of reporter gene Gus was observed in F2,BC1 and BC1F2 progenies.Thus indicated that transgenes inherited as a single dominant gene in the progenies of Bt rice crossed to conventional rice varieties.
【Fund】: 浙江省科委“九五”重大项目;; 洛氏基金资助!项目(RF97001#556)
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
1 CHEN Wen-yue1,GUAN Shi-liang2,YU Xiang-qun1,SONG Liang1,YING Wu1(1.Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China; 2.Seed Company of Chun'an County,Chun'an 311700,China);Strategy and Progress of Breeding Insect-resistant Bt Transgenic Japonica Rice[J];Acta Agriculturae Jiangxi;2007-10
2 ZHAO Mei-xiang1,HUANG Jie1,2,XIA Rui-qiang1,XIAO Ning1,HONG Yi-huan1,CHEN Jian-min1(1.College of Bioscience and Biotechnology,Yangzhou University,Yangzhou,Jiangsu 225009,China; 2.Agricultural Technology Extension Center of Suzhou,Suzhou,Jiangsu 215000,China);Inheritance and Expression of Green Fluorescent Protein Gene(gfp) in Transgenic Barley(Hordeum vulgare L.)[J];Journal of Triticeae Crops;2008-03
3 Chen Yejian 1) Shu Qingyao 2) Wu Qibao 3) Zhang Zengqing 1) Liu Shouping 1) He Fangyin 1) ( 1)Taizhou Agricultural Research Academy of Zhejiang Province, Linhai 317000; 2)Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou 310029; 3)Sanmen Seed Station,Sanmen 317100);SUPERIORITY OF Bt GENE IN HYBRID RICE PROGENIES[J];Acta Agriculturae Shanghai;2004-03
4 WANG Jun\|hui 1 ZHANG Jian\|guo 1 HU Jian\|jun 1 ZHANG Zhen 2 ZHANG Shou\|gong (1 Research Institute of forestry Chinese Academy of Forestry, Beijing\ 100091, China) (2 Institute of Forest Ecology Environment and protection Chinese Academy of Forestry, Beijing\ 100091 China);Studies on Safety Assessment of Transgenic Bt Poplar[J];Progress In Biotechnology;2004-01
5 Wang Nian Wang Junhui Zhang Jianguo Zhang Shougong (Research Institute of Forestry,Chinese Academy of Forestry, Beijing 100091);Current Development of Transgenic Plants and the Segregation of Transgene in the Progenies of Transgenic Plants[J];Biotechnology Information;2004-01
6 Li Weifeng1,2 Yang Lang3(1Guangxi Entry-Exit Inspection & Quarantine Bureau,Nanning 530028;2Lab.of Insect ecology,South China agricultural University,Guangzhou 510642;3Guangxi Crop Genetic Improvement and Biotechnology Lab,Guangxi Academy of Agricultural Sciences,Nanning 530007);Current Development of Detecting Methods in Transgenic Rice[J];Biotechnology Bulletin;2006-05
7 XIE Xiao Bo ①,CUI Hai Rui, SHEN Sheng Quan, WU Dian Xing, XIA Ying Wu, SHU Qing Yao ② (Laboratory of Mutation Genetics and Plant Molecular Improvement, Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310029, China);Studies on the Distorted Segregation of Foreign Genes in Transgenic Rice Progenies[J];Acta Genetica Sinica;2002-11
8 WANG Zhong-hua, SHU Qing-yao, YE Gong-yin, GUI Hai-rui, XIA Ying-wu( Institute of Nuclear Agricultural Sciences , Zhejiang University, Hangzhou 310029; Department of Plant Protection , Zhejiang University, Hangzhou 310029);Genetic Analysis of Resistance of Bt Rice to Stripe Stem Borer (Chilo suppressalis)[J];Scientia Agricultura Sinica;2002-01
9 WANG Lei,QU Yue-Jun,DU Ren-Jie,WU Li-Li,WANG Yu-Cheng,JIANG Ting-Bo Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northest Forest University,Harbin 150040,China;Identification of MT Overexpression in Tobacco and Analysis of Cd~(2+) Resistance in Transgenic Plants[J];Plant Physiology Communications;2009-04
Chinese Journal Full-text Database 2 Hits
1 Xiang Youbin 1 Liang Zhuqing 1 Gao Mingwei 1 Shu Qingyao 1 Ye Gongyin 2 Cheng Xiongying 1,3 I.Altosaar 3 1(Institute of Nuclear Agricultural Sciences,Zhejiang Agricultural University,Hangzhou 310029) 2(Department of plant pr;Agrobacterium mediated Transformation of Insecticidal Bacillus thuringiensis cryIA(b) and cryIA(c) Genes and Their Expression in Rice[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1999-04
2 Li Yane Jiao Gaili Li Shujun Fan Xiaoping Wu Xia Chen Zhixian (Institute of Cotton, Shanxi Academy of Agricultural Sciences, Yuncheng 044000) Guo Sandui (Biotechnology Research Center, Chinese Academy of Agricultural Sciences) Zhu Zhen (I;Obtaining of Homozygous Transgenic Cotton Lines and Their Genetic Analysis[J];SCIENTIA AGRICULTURA SINICA;1998-05
Chinese Journal Full-text Database 10 Hits
1 Xiang Taihe et al (Rice Research Institute and Key Laboratory of Rice Genetics and Breeding of Agricultural Ministry,Anhui Academy of Agricultural Sciences,Hefei 230031);Preliminary Study on Transferring Antibacterial Polypeptide Shiva A Gene into Restorer Line Minhui 63 of Hybrid Rice Via Agrobacterium mediated Gene Transformation System[J];Journal of Anhui Agricultural Sciences;1999-06
2 YUAN Zhi-dong,YAO Hong-wei,YE Gong-yin,HU Cui(Institute of Insect Sciences /State Key Laboratory of Rice Biology,Zhejiang University,Hangzhou 310029,China);Survival Analysis of the Larvae from Different Hybrids of Silkworm,Bombyx mori Exposure to Bt Rice Pollen[J];Bulletin of Sericulture;2006-03
3 HU Fan-rong~(1,2) (1.Institute of Nulear Agricultual Sciences, Zhejiang University, Hangzhou 310029, China; 2.Bioengineering School, Jinhua College of Profession and Technology, Jinhua 321007, China);Preliminary study on major factors for tissue culture and genetic transformation of Japanese lawngrass ( Zoysia japonica Steud.)[J];Journal of Agricultural University of Hebei;2004-02
4 JIANG Cai-Ying1,2 YAO Hong-Wei2 YE Gong-Yin2 HU Cui2(1College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China;2State Key Laboratory of Rice Biology,Institute of Insect Sciences,Zhejiang University;Hangzhou 310029,China);Effect of Sunlight on Content and Activity of Insecticidal Protein in the Pollen of Transgenic Bt Rice[J];Science of Sericulture;2007-04
5 ZHANG Lei~(1,2), WU Dian-xing~1, HU Fan-rong~1, WANG Hai-qiu~1, MA Chuan-xi~2(1. Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 2. Agronomy Department, Anhui Agricultural University, Hefei 230036, China);Optimization of major factors for tissue culture and Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica)[J];Acta Pratacultural Science;2004-04
6 Liu Wuge 1* Xue Qingzhong 2 1 Rice Research Institute, Guangdong Academy of Agricultural Science, Guangzhou, 510640 2 Department of Agronomy, Zhejiang University, Hangzhou, 31002 *Corresponding auther,liuwuge@21cn com;Study on Expression Characteristics of CpTI Gene in Transgenic Plants of Rice[J];Molecular Plant Breeding;2003-02
7 Li Aihong1,3* Dai Zhengyuan1 Shu Qingyao2 Zuo Shimin3 Liu Guangqing1 Shen Shengquan2 Kong Xiangdou1 Zhang Hongxi11 Lixiahe Agricultural Science Institute of Jiangsu Province, Yangzhou, 2250072 Institute of Nuclear Agricultural Science, Zhejiang University(Huajiachi Campus), Hangzhou, 3100293 Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, 225009;Marker Assisted Selection and Evaluation for its Resistance to Striped Stem Borers and Leaf Folders in Progenies of Rice Germplasm Transferred with Cry1Ab Gene[J];Molecular Plant Breeding;2004-04
8 Lv Yan1 Wang Pingrong2 Sun Yeying2 Dong Chunlin2 Chen Dexi2 Deng Xiaojian2*1 College of Chemistry Biology and Technology, Yantai University, Yantai, 264005; 2 Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130;Application of Agrobacterium-mediated Transformation in Rice Genetic Engineering[J];Molecular Plant Breeding;2005-04
9 Jin Wanmei1,2* Pan Qinghua2 Yin Shuping2 Dong Jing2 Jiang Lijie2 Yang Li2 Chen Qinghua2 Zhao Jianbo2 1 College of Agricultural and Biotechnology Science, China Agricultural Universi ty, Beijing, 100094; 2 Institute of Forestry and Pomology, Beijing Academy of Ag riculture and Forestry Science, Beijing 100093;Progress of the Genetic Stability and Breeding Behavior of Foreign Gene in Genetically Modified Plants[J];Molecular Plant Breeding;2005-06
10 Lin Xiufeng1 Liu Zhiming1 Liu Depu1* Hao Wenyuan1 Tang Kexuan2 Wu Tianlong31 Biotechnology Research Center, Jilin Academy of Agriculture Science, Gongzhuling, 136100; 2 State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433; 3 School of Agriculture and Biology, Shanghai Jitong University, Shanghai, 201101;Introduction Double Insect Resistance Genes CryIA(a)-pta to Japonica Rice and Assessment of Resistance to Rice Stem Borer[J];Molecular Plant Breeding;2006-03
China Proceedings of conference Full-text Database 7 Hits
1 Jiao Xiaoguo Zhang Guoan Tu Jumin Cui Xuhong (Central China Agriculture University,Wuhan,China 430070);Bioassay on resistance to pests of the transgenic Bt rice[A];[C];2002
2 Wang Cai-lin Zhao Ling Zhu Zhen Zhang Ya-dong Lin Jing Zhang Suo-bing Chen Tao Liu Xian-jin Wang Dong-Ian Huang Jun-qi (Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) Gong Zhen-zhen (Shanghai Institute of Biochemistry and cell Biology, Academy Sinica, Shanghai, 200031, China);Transgenic Rice Plant Transformed by Introduction of Arrowhead Proteinase Inhibitor (API) Geneinto Rice by Pollen-tube Pathway Method[A];[C];2004
3 JIAO Gai-li, NAN Zhi-Run, ZHANG Huan-yang, WANG Jiao-juan, ZHAO Jun-xia (Institute of Cotton, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China);Study on the Regeneration of Transgenic Colored Cotton Plants by Agrobacterium-mediated[A];[C];2004
4 Jingyu Wang;Xiaofu Zhou;Xiuyun Lin;Mingsheng Li;Chenghai Jin;Guicai Wang Rice Institute, Jilin Academy of Agricultural Sciences Gongzhuling 136100 Gene Engineering Institute, Jilin Normal University Siping 136000;Setting Up of Japonica Rice Transformation System Via Biolistic[A];[C];2003
5 WANG Hai-yan, YE Qing-fu;Adsorption, Desorption and Biodegradation in Soil of Cry1Ab Toxin Protein from Bt Transgenic Rice[A];[C];2004
6 Zhang Qijun Li Cong Yu Derong Sun Yonghua LU Chuangen Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing Branch of National Rice Improvement Center,Jiangsu High Quality Rice R&D Center,Nanjing,210014;Innovating new Transgenic Wide Compatible Rice Resource Harboring Disease-Resistant and Insecticidal Genes[A];[C];2010
7 ZHUO,Chunliu,CAI Jiongliang,FANG Junyang,ZHOU Ming,GUO Zhenfei (Biotechnology Laboratory for Turfgrass and Forages,Department of Plant Science,College of Life Sciences,South China Agricultural University,Guangzhou 510642:China.);Early light induced protein(ELIP) gene from Medicago falcata confers resistance to abiotic stresses[A];[C];2010
Chinese Journal Full-text Database 10 Hits
1 WANG Jia-bao,WANG Liu-ming,SHEN Fa-fu,ZHANG Xue-kun,YANG Jing,LIU Ren-zhong (Shangdong Cotton Research Center,Jinan 250100,China);Effect of environment elements on Bt-protein content in transgenic Bt cotton[J];Shandong Agricultural Sciences;2000-06
2 LI Ru-zhong 1,SHEN Fa-fu 2,WANG Zong-wen 1,WANG Jing-hui 1,LIU Cheng-yun 1,SHEN Gui-fang 1 (1 Shandong Cotton Research Center, Jinan 250100,China;2 Shandong Agricultural University, Taian 271018,China);Temporal and spatial dynamics of Bt gene expression in Bt transgenic cotton[J];Shandong Agricultural Sciences;2002-02
3 Shan Lei,Bi Yuping,Wang Xingjun,Xu Pingli & Li Guangcun (Biotechnology Research Center,Shangdong Academy of Agricultural Sciences,Jinan 250100);Genetic Analysis on the Progeny of the Transgenic Tomato with Double-resistance to TMV and CMV[J];SHANDONG AGRICULTURAL SCIENCES;1998-04
4 Zhou Dongsheng et al (Department of Plant Protection,Anhui Agricultural University,Hefei 230036);Chamber Evaluation Technology of Insect resistance of Bt Transgenic Cotton[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;2000-05
5 TANG Wei(Institute of Basic Medicine,Yunyang Medical College,Shiyan, Hubei 442000);Inheritance Analysis of Progenies of Several Transgenic Plants[J];Journal of Anhui Agricultural Sciences;2007-31
6 YUAN Sheng-liang et al(Hebei Agricultural University,Baoding,Hebei 071000);Brief Analysis on the Insect-resistant Genes Species and Their Insect-resistant Principles[J];Journal of Anhui Agricultural Sciences;2007-31
7 ZHOU Dong sheng 1,WU Zhen ting 1,WANG Xue lin 1,NI Chun geng 1, ZHENG Hou jin 2,XIA Jing 2 (1 Department of Plant Protection, Anhui Agricultural University,Hefei 230036; 2 The General Seeds Company of Anhui Province);Influence of Fertilization and Environmental Temperature on the Resistance of Bt Transgenic Cotton to Cotton Bollworm[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-04
8 LIN Yi (Department of Bioengineering,Anhui Agricultural University,Hefei 230036);Risk Assessment of Bar Gene Transfer from B and D Genomes of Transformed Wheat (Triticum aestivum) Lines to Jointed Goatgrass (Aegilops cylindrica)[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-02
9 YU Zhihua CHEN Minyong\ GU Hongya\ PAN Naisui\ CHEN Zhangliang (College of Life Sciences, Peking University, Beijing, 100871) LI\ Yan (Dandong Academy of Agriculture Sciences, Liaoning, 118109);Upland Rice Transformation[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1996-04
10 Wang Zezhou Qiu Quansheng (College of Life Sciences, Beijing Normal University, 100875, Beijing, China);THE TRANSIENT EXPRESSION OF MGFP4 IN RICE CALLI[J];JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE);2000-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Dong-peng,YU Li-jie(College of Biological Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025);Establishment of Tomato Transformation Technique Via the Pollen tube Pathway Method[J];Northern Horticulture;2010-14
2 YAN Li-ping1,XIA Yang1,LIANG Hui-min2,WANG Zhen-zhen1,LI Shuang-yun1,LIU Cui-lan1,PANG Cai-hong1(1.Shandong Provincial Academy of Forestry,Jinan 250014,China;2.Jiangsu Agriculture and Forestry Profession Technology College,Jurong 212400,China);A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene[J];Acta Prataculturae Sinica;2009-06
3 CUO Yushuang, ZHU Yanming,LI Jie,BAI Xi,CAI Hua (College of Life Sciences, Northeast Agricultural University, Harbin 150030, China);Investigation and analysis of the main agronomic traits of transgenic soybean with two anti-fungal protein genes[J];Journal of Northeast Agricultural University;2008-01
4 DU Tao,GUO Jian-hua,ZHANG Hai-zhou,HOU Ming-sheng, HUANG Xiao-mao,ZHOU Ning-yi,LIN Mu-lan(Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071 ,China;College of Plant Science and Technology, Huazhong Agricultural University,Wuhan 430071 ,China;Xuchang Institute of Forest ,Xuchang,He'nan 461000,China);Gene shiva-1 Inheritance and Expression of Transgenic Paulownia[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
5 Li Aihong1,3* Dai Zhengyuan1 Shu Qingyao2 Zuo Shimin3 Liu Guangqing1 Shen Shengquan2 Kong Xiangdou1 Zhang Hongxi11 Lixiahe Agricultural Science Institute of Jiangsu Province, Yangzhou, 2250072 Institute of Nuclear Agricultural Science, Zhejiang University(Huajiachi Campus), Hangzhou, 3100293 Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, 225009;Marker Assisted Selection and Evaluation for its Resistance to Striped Stem Borers and Leaf Folders in Progenies of Rice Germplasm Transferred with Cry1Ab Gene[J];Molecular Plant Breeding;2004-04
6 Wang Lei Yu Lijie* Ling Chunying Li Ranhong Department of Biology,College of Life and Environment Science,Harbin Normal University,Harbin,150025;Primary Study on Transgenic Chinese Cabbage with Interleukin-4 Gene via Pollen-tube Pathway[J];Molecular Plant Breeding;2006-06
7 LI Xiao-yong1,TANG Hai-yan2,YANG Xin-qing1,LI Rong-bai1(1.Rice Research Institute of GXAAS,Nanning 530007,China;2.Hejiashan Foundation Seed Farm of Changde City,Hunan Province,Changde 415123,China);Comparison of agronomic traits among progenies derived from double-transgenic (Bt/NPR1) hybrids and their resistance identification[J];Guangxi Agricultural Sciences;2008-02
8 CAI Hua1,ZHU Yanming1,GUO Yushuang2,BAI Xi1,LI Yong1,JI Wei1(1.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China;2.Guizhou Tobacco Research Institute,Guiyang 550081,China);Analysis of genetic stability of transgenic soybean with two anti-fungal protein genes[J];Journal of Northeast Agricultural University;2011-07
9 GU Ya-nan,ZHAO Yong-liang,WANG Dan,CHEN Jing (Bioengineering Department,Henan University of Technology,Zhengzhou 450001,China);Development of the Identification of the Target Protein in Transgenic Plant Research[J];Journal of Henan Normal University(Natural Science);2010-06
10 WANG Zhong-hua~1 ZHANG Li-cheng~(1,2)(1 Institute of Biotechnology,Zhejiang Wanli University,Ningbo,Zhejiang 3151002 Agricultural Office,Government of Baifeng Town in Beilun Division,Ningbo,Zhejiang 315000);CURRENT RESEARCH TRENDS OF BREEDING APPLICATION OF BT TRANSGENIC RICE[J];Journal of Nuclear Agricultural Sciences;2007-02
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Chen Zhixian Li Shujun (Cotton Res.Ins., Shanxi Acad. of Agri. Sci., Yucheng) N.L.Trolinder (Cropping Systems Res. Lab., USDA-ARS, Lubbock, Texas 79409) J.R.Goodin (Texas Tech. University, Lubbock, Texas 79409);SOME CHARACTERISTICS OF SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN UOTTON CELL SUSPENSION CULTURE[J];Scientia Agricultura Sinica;1987-05
2 Clen Zhixian;Danny J.Llewllyn;Fan Yunliu;Li Shujun;Guo Sanduei;Jiao gaili;Zhao Junxia(1.Institute of Cotton, Shanxi Academy of Agricultural Sciences,Yuncheng,Shanxi,044000,China)(2.CSIRO Division of Piant Industry,Canberra,ACT2601,Australia)(3.Biotechnolo;2,4-D RESISTANT TRANSGENIC COTTON PLANTS PRODUCED BY AGROBACTERIUM-MEDIATED GENE TRANSFER[J];SCIENTIA AGRICUTURA SINICA;1994-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved