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《Hereditas(Beijing)》 2001-05
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Genetic Monitoring of Genomic DNAs from Guizhou Miniature Pigs BY Aflp

WU Feng chun 1,WEI Hong 1,GAN Shi xiang 2,ZHOU Jian hua 1,MA Jing 1 ( 1. Laboratory Animal Center, Third Military Medical University,Chongqing 400038,China ; 2. Guiyang Traditional Chinese Medical College ,Guiyang 550002 ,China)  
We reported the application of AFLP markers to detect genetic polymorphic loci in Guizhou miniature pig and their amplified AFLP results.Their genetic construction of the population was also analysed.TenAFLP primers were used,genomic DNAs from 17 pigs were restrictive by pstⅠ,116 AFLP markers were obtained,the obtaining marker numbers of individual primer were between 2~22.The results indicate as the following:(1)AFLP marker is suitable for analysing genetic polymorphism in pig;(2)The similarty index of population in Guizhou miniature pigs was 0.866(0.760~0.967).The study provides a useful parameter with appraise genetic stability of Guizhou miniature pigs.
【Fund】: 国家重点基础研究发展规划项目 ( 2 0 0 0 16 10 6 ) ;; 国家“九五”重点科技攻关项目 ( 96A -2 3-0 6 -0 1);; 国家自然科学基金重点项目 (NO39830 190 );; 面上项目 ( 39770 398)资助
【CateGory Index】: S828.1;Q503
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