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Genetic Analysis and Identification of Three Groups Three-line Hybrid Rice and Their Parents by RAPD Markers

MAO Jia ning,DUAN Shi hua, LI Shao qing,ZHU Ying guo (The Key Laboratory of MOE for Plant Development Biology and Institute of Genetics, College of Life Sciences,Wuhan University,Wuhan 430072,China)  
A total of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) techniques with the genome DNA of three groups of three-line hybrid rice and their parents.Thirteen primers produced 43 polymorphism fragments.Six primers of them produced 20 obviously repeatable polymorphic markers among rice lines tested.Using this RAPD markers,the hybrid rice combinations (sterile-line,maintainer-line,restorer-line and F 1)can be effectively identified,and the genetic relationship among them can be shown.
【Fund】: 国家自然科学基金 (批准号 :39770 45 5 )
【CateGory Index】: S511.01
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