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《Hereditas(Beijing)》 2002-03
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Introduction of a Simple and Effective Method for Plant Totle RNA Isolation

ZHAO Shuang yi,WU Yao rong,XIA Guang min (School of Life Sciences,Shandong University,Jinan 250100,China)  
Wheat leaf and seeds at different development stages had been squashed in liquid nitrogen,then lysised by urea buffer which contains 0.1% SDS and 0.1% LDS,denatured protein had been removed by NaAc and chloroform precipitation,total RNA was further purified by LiCl.The RNA we obtained had sharp bands of 28S and 18S after agarose gel electrophoresis,23S and 16S RNA bands can also be seen clearly in leaf RNA extract,the value of OD 260 /OD 280 of RNA was 2 05~2 10.5mg RNA can been isolated from 10g leaf of wheat.This method can also been used in high molecular weight DNA isolation but the concentration of SDS and LDS must be increased to 1%.
【Fund】: 国家转基因专项 (J99-A -0 37)农杆菌介导小麦转化技术的研究
【CateGory Index】: Q943
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