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《Hereditas》 2006-08
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PCR-RFLP Analysis of the mtDNA Cytb Gene in Three Different Horse Breeds

LI Jin-Lian,SHI You-Fei,BU Ren-Qiqige,MANG Lai(College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China)  
Restriction endonucleases,namely BamHⅠ,TaqⅠ,HaeⅢ,RsaⅠand HincⅡ,were used to analyze the polymorphism of partial mtDNA Cytb gene sequences from 256 horses 6 types(Thoroughbred,Sanhe,Wuzhumuqin,Xinihe,Wushen and Pony)including the imported breed,cultivated breed and local breed.The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.Results indicated BamHⅠ and TaqⅠ polymorphism.In all,7 restriction patterns were defected that could be sorted into 3 haplotypes,of which haplotypes Ⅰ and Ⅲ were the basic haplotypes.We infer that these horses came from one female ancestor possibly through the analysis thorough one pattern,namely BamHⅠ-B.
【Fund】: 国家教育部科学技术研究重点项目(编号:01018)资助~~
【CateGory Index】: S821.2
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