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《Hereditas》 2008-02
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Localization of nucleolar DNA and transcription sites of rRNA genes in situ in wheat cells

HE Jie 1 ,TAO Wei 2 ,HAO Shui 3 1.Department of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China; 2.College of Life Sciences,Peking University,Beijing 100871,China; 3.Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China  
By using the conventional electron microscopic technique and DNA specific cytochemical staining method (NAMA-Ur),we directly observed the arrangement and location of intranucleolar DNA in situ in wheat(Triticum aestivum L.)cells.The results showed that nucleolar DNA was found in Fibrillar Centers(FC),Dense Fibrillar Component(DFC) and the transitional region between FC and DFC.Moreover,the nucleolar DNA was distributed along the periphery of FC and by surrounding FC.We employed RNP preference staining(Bernhard staining)method to visualize the distribution and position of RNP in situ in nucleoli of wheat cells.The results directly showed that RNP mainly located in the transitional region between FC and DFC,in DFC and in Granular Component(GC).Moreover,RNP was irregularly distributed around FC.By employing anti-RNA/DNA hybrid antibodies,we directly and selectively labeled transcription sites of rRNA genes and testified that localization of transcription sites was not only in the transitional region between DFC and FC but also inDFC of nucleoli in wheat cells.
【Fund】: 国家自然科学基金(编号:39770367)资助~~
【CateGory Index】: Q943;S512.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 HE Jie TAO Wei (Department of Biotechnology, Harbin Institute of Technology, Harbin 150001) (College of Life Sciences, Peking University, Beijing 100871);ULTRASTRUCTURAL LOCALIZATION OF TRANSCRIPTION SITES OF rRNA GENES IN THE NUCLEOLUS OF WHEAT[J];Acta Biologiae Experimentalis Sinica;2002-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LU Xian ping 1, LI Bao jin 2, CHEN Sheng le 2, et al 1Department of Nuclear Medicine and 2Department of Surgery, Memorial Hospital, Sun Yat sen University of Medical Sciences,Guangzhou 510120,P R China;Effect of proliferous kinetics of targeting chemotherapy on colorectal carcinoma cells[J];CHINESE JOURNAL OF CANCER;2000-04
2 ZHANG Liyong,SUN Haijing,JIAO Mingda(Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China);Nucleolus ultrastructure and distribution sites of rDNA of rat liver cells[J];Journal of Northeast Normal University (Natural Science Edition);2002-04
3 ZHANG Fei-xiong,PENG Li (Department of Biology,Capital Normal University,Beijing 100037,China);Electron microscopic observation of nucleolar channels in root-tip meristematic cell nuclei of common wheat[J];Acta Botanica Boreali-occidentalia Sinica;2002-01
4 YIN Jiang mei,ZHANG Fei xiong * (Department of Biology, Capital Normal University, Beijing 100037,China);Ultrastructure of the nucleolus and the location of rDNA replicating in root-tip meristematic cells of Triticum aestivum L.[J];Journal of Chinese Electron Microscopy Society;2003-01
5 Amrutlal K. Patel1,2, Doug Olson3, and Suresh K. Tikoo1,2,4* 1Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada; 2Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada; 3National Research Council, Plant Biotechnology Institute, University of Saskatchewan, Saskatoon, Canada; 4School of Public Health, University of Saskatchewan, Saskatoon, Canada.;Proteomic Analysis of Bovine Nucleolus[J];基因组蛋白质组与生物信息学报(英文版);2010-03
6 Amrutlal K. Patel~(1,2), Doug Olson~3, and Suresh K. Tikoo~(1,2,4*) 1 Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada; 2 Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada; 3 National Research Council, Plant Biotechnology Institute, University of Saskatchewan, Saskatoon, Canada; 4 School of Public Health, University of Saskatchewan, Saskatoon, Canada.;Proteomic Analysis of Bovine Nucleolus[J];基因组蛋白质组与生物信息学报(英文版);2010-03
7 TAO Wei, HE Jie, JIAO Mingda, HE Mengyuan & HAO ShuiDepartment of Cell Biology, College of Life Sciences, Peking University, Beijing 100871, China; Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;Configuration of nucleolar DNA in situ in nucleolus of Allium cepa cells[J];科学通报(英文版);2001-01
8 Liu Yue; Fu Qingji ;Zheng Hongzhu(Department of Urology, the 210th Hospital of PLA,Dalian 116021);Expression of AgNOR in renal pelvis cancar and its clinical significance[J];JOURNAL OF CLINAICAL UROLOGY;1997-01
9 Liu Yue; Fu Qingji; Wang Lixin et al(Department of Urology,the 210th Hospital of PLA,Dalian, 116021 );Expression of AgNOR and PCNA in benign and malignant prostatic lesions[J];Journal of Clinaical Urology;1998-10
10 Xu-Xu Sun, Jing Yi* (Department of Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China);Nucleolar Proteins: Their Location, Translocation and Nucleolar Functions[J];Chinese Journal of Cell Biology;2008-05
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