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《Journal of Agricultural Science Yanbian University》 2009-02
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Rapid detection of PSTVd using RT-PCR

KANG Zhe-xiu1, JIN Shun-fu1,JIANG Cheng-mo1,XUAN Chun-ji1,LIU Xian-hu2(1.Academy of Agricultural Sciences Institute of Yanbian;2.Agricultural College of Yanbian University:Longjin Jilin 133400,China)  
A pair of RNA primer was designed and synthesized based on the specific sequence of the gene of PSTVd.The total RNA with high purity and quality was obtained from the virus-infected potato tissue by using the improved method.The RNA could be used for cDNA synthesis and polymerase chain reaction(PCR).A specific PCR fragment about 359 bp was obtained from the virus-infected tissue,which was just in accord with the theoretical design,while no amplified products were obtained from the healthy tissue.
【CateGory Index】: S532
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