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《ACTA UNIVERSITIS MEDICINALIS NAHUI》 1999-06
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Establishment of necrotic injured models of primary hepatocytes from rats

Yang Yan, Chen Minzhu (Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032)  
Objective To establish the nectotic injured models of hepatocytos in vitro for further studying protective effects of drugs on injured hepatocytes. Methods The hepatocytes were isolated by perfusion of liver with Ⅳ type collagenase in rats and incubated with CCl 4 or H 2O 2. The necrotic hepatocytes induced by CCl 4 or H 2O 2 were investigated by measuring the levels of AST and ALT, the amount of MDA and the activity of GSHpx, and MTT colormetry. Results The most optimal injured conditions of CCl 4 or H 2O 2 were separately determined as CCl 4: 8 mmol·L -1 , 6 h; H 2O 2: 0 6 mmol·L -1 , 1 h. Conclusion Two kinds of necrotic injured models of hepatocytes may be created separately by the most optimal injured conditions of CCl 4 or H 2O 2.
【Fund】: 安徽省教委课题基金!资助 (编号 :95JL0 0 69
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