Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Establishment of necrotic injured models of primary hepatocytes from rats

Yang Yan, Chen Minzhu (Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032)  
Objective To establish the nectotic injured models of hepatocytos in vitro for further studying protective effects of drugs on injured hepatocytes. Methods The hepatocytes were isolated by perfusion of liver with Ⅳ type collagenase in rats and incubated with CCl 4 or H 2O 2. The necrotic hepatocytes induced by CCl 4 or H 2O 2 were investigated by measuring the levels of AST and ALT, the amount of MDA and the activity of GSHpx, and MTT colormetry. Results The most optimal injured conditions of CCl 4 or H 2O 2 were separately determined as CCl 4: 8 mmol·L -1 , 6 h; H 2O 2: 0 6 mmol·L -1 , 1 h. Conclusion Two kinds of necrotic injured models of hepatocytes may be created separately by the most optimal injured conditions of CCl 4 or H 2O 2.
【Fund】: 安徽省教委课题基金!资助 (编号 :95JL0 0 69
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 LI Li1,YU Hongru1,WANG Hongxin2,YANG Jing2 (1.Liaoning Medical University;2.Research Institute of Pharmacy,Liaoning Medical University,Jinzhou 121000 China);The Effect of Calf Liver Extract on the Damage of the Neonatal Mice Hepatocytes[J];Journal of Liaoning Medical University;2008-02
2 SHEN Hong,GU Fuping,ZHANG Bei,et al.Medical Department,Huzhou Teachers College,Huzhou 313000,CHINA;Comparison of the protective effects of different extracts of ginger oil on hepatocytes injury induced by hydrogen peroxide in vitro[J];Jiangsu Medical Journal;2009-08
3 YANG Yan, CHEN Min-Zhu (Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, China);Inhibitory effect of total extract of Astragalus on hepatocyte apoptosis[J];Chinese Journal of Pharmacology and Toxicology;2001-04
4 YANG Yan CHEN Min-Zhu(Institute of Clinical Pharmacology, Anhui Medical University,Hefei 230032);Protective effects of total extract of astragalus(TEA) onprimary rats hepatocytes injured by CCl4 or H2O2[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2000-04
5 OU Yu,ZHENG Shan,LIN Lin(School of life science and technology,China Pharmacutical University,Nanjing,210009 China);Protective Effects of C-Phycocyanin on Hepatoma Cell HepG2 Injury in vitro[J];Pharmaceutical Biotechnology;2009-05
6 YAN Bing1,2,DING An-wei1,ZHANG Li1(1.Jiangsu Key Laboratory for TCM Formulae Research,N anjing University of Chinese Medicine,Nanjing 210046,China;2.Dept.of Pharmaceuticals,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,China);Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl_4 or H_2O_2[J];Journal of Pharmaceutical Practice;2010-06
7 LANG Guiling(The Friendship Hospital of Dalian,Dalian116001,China) ZHOU Qin,LI Weiping,JIN Zhao,YU Yang,HAN Guozhu(Dalian Medical University,Dalian116023,China);Protective Action of Tea Polyphenol on HepG2 Cells[J];China Pharmacy;2006-09
8 DUAN Xiaoqun,LIN Xing,JIAO Yang,HUANG Renbin(Dept.of Pharmacology,Guangxi Medical Univer-sity,Nanning530021,China);Protection of Longyanshen Polysaccharide on CCl 4 -induced Damage of Primary Cultured Hepatocytes in Rats[J];China Pharmacy;2006-15
9 JIAO Yang,DUAN Xiao-qun,LIN Xing,HUANG Ren-bin (Department of Pharmacology,Guangxi Medical University,Guangxi Nanning 530021,China);Protective effects of Yulangsan polysaccharide on primary cultured rat hepatocytes injured by CCl_4[J];Chinese Journal of Hospital Pharmacy;2006-11
10 LI Guo-li,WEI Hong-shan,ZHANG Jian-ping,XIAO Fan,HAN Jun-yan,LIU Zhi-ying,FAN Wen-mei,LIU Shun-ai,ZENG Hui.Institute of Infectious Diseases,Beijing Ditan Hospital,Beijing 100011,China;The protection of panax notoginseng saponins against liver damage and its potential mechanisms[J];Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version);2007-01
Chinese Journal Full-text Database 2 Hits
1 Rong Zhengxing, Liu Huizhong, Bao Jingqi, ChenHongzhuan, Sun Yuyan. Sun Chen. (Dpart-ment of Pharniacology. Shanghai 2nd MedicalUniversity, Shanghai 200025 ) .;Direct Micro Determination of GlutatbionePeroxidase Activity in Mice Blood.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1994-04
Chinese Journal Full-text Database 10 Hits
1 SUI Hai-xia, GAO Peng, XU Hai-bin, et al (National Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China);A Rapid Toxic Screening Experiment on Duzhong[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-06
2 WAN Mei-rong 1 ,ZHU Hong-hu 1 ,CHEN Shao-zhi 2 (1.Central Laboratory,Allilisted Hospital,Xuzhou Medical College,Xuzhou Jiangsu221002,China; 2.Xuzhou Children's Hospital,Xuzhou Jiangsu 221000,China);The Feasibility for Screening Anticancerous Drug Using Human Leukemic Cell Lines[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-02
3 SUI Hai-xia,GAO Peng,XU Hai-bin(National Institute of Nutrition and Food Safety, China CDC, Beijing 100021);The Safety Evaluation of Herba Epimedii Water Extract[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-06
4 SUN Xuan,XIA Xin-hui,LIAO Yu-fen, ZHANG Dong-fang,WANG Gu-xin,LUO Gang (Department of Urology,Futian People's Hospital,Shenzhen 518033,Guangdong,China);Induction of specific CTL against bladder cancer by MUC1 gene-modified dendritic cells transfected with dextran coated magnetic iron oxide nanoparticles[J];Carcinogenesis,Teratogenesis & Mutagenesis;2011-02
5 Yang Ruihua,Hai Chunxu,Li Ruizhen,Wu Duanzong Department of Military Hygiene,the Forth Military Medical University ,Xi'an 710032;DOSE-RESPONSE RELATIONSHIP BETWEEN CELL PROLIFERATION AND HYDROGEN PEROXIDE[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-02
6 Xininigen et al(College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018);Cloning and Expression of IL-2 Gene from Holstein-Friesian Cow[J];Journal of Anhui Agricultural Sciences;2008-35
7 Zhang Mei,Xia Tian,Zhang Zhonghai,Hang Li,Xu Chongmin (Dept.of TCM XiJing Hospital,The 4th Militery Medical University,Xi'an 710032);STUDY OF SIL-2R AND LYMPHOCYTE PROLIFERATIVE ACTIVITY IN PATIENTS WITH SPLEEN ASTHENIA[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;1999-06
8 Chen Yong,Wu Minyu (Department of Microbiology and Immunology of Bengbu Medical College,Bengbu 233003,China);EFFECT OF FANGJI HUANGQI DECOCTION ON Mφ AND T CELL FUNCTION OF SPLEEN ASTHENIA MICE[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;2000-01
9 ZHU Xiao feng, ZHANG Rong hua,PENG Ke ping(The First Hospital of Ji′nan University,Guangdong Guangzhou 510630, China);Effect of Yigu Capsule Drug-containing Serum onIGF-Ⅰ Secretion by Osteoblasts in Vitro[J];Journal of Anhui Traditional Chinese Medical College;2003-02
10 SHEN Guo ming, XU Ying,L Lei,WANG Wang jiu, XU Guan sun (Departerment of Integrated Traditional Chinese and Western Medicine, Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);Effect of Electro-acupuncture on the Immunoreactivity of Vasoactive Intestinal Peptide in Gastric Mucosal Injury Rats[J];Journal of Anhui Traditional Chinese Medical College;2004-03
China Proceedings of conference Full-text Database 1 Hits
1 Wu Fengzhi, Jiao Xiaodan and Yu Xihong (Northeast Agricultural University, Harbin 150030, China);Effects of Cinnamic Acids on the Antioxidant Enzymes and Proline of Cucumber Seedlings by Soil Culture[A];[C];2004
Chinese Journal Full-text Database 10 Hits
1 MA Ke-xue et al(College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007);Progress in Models of Animal Liver Regeneration[J];Journal of Anhui Agricultural Sciences;2009-13
2 ZOU Hao- jun,MING Liang;Progress of studies on anti-inflammation, immunodulatory and anti-senility effects of total flavones[J];Journal of Anhui Health Vocational & Technical College;2003-06
3 GAO Chang Kun, GAO Jian, MA Ru Long 1, XU Xian Xiang 1, HUANG Peng 1, NI Shou Dong 1 (Dept of Phar, The First Affliated Hospital of Anhui Medical University,Hefei 230022; 1Anhui College of Traditional Chinese Medicine,Hefei 230038);Research on analgesic and anti-inflammatory and invigorate circulation effects of Total Saponins of Achyranthes[J];Anhui Medical and Pharmaceutical Journal;2003-04
4 Jiang Congqiao,Ling xianzhao,Lu Zheng,Ge Xin,Zhong Zhengrong,Xie Qun,Yang Yongkang (Department of General Surgery,Affiliated Hospital of Bengbu Medical College,Anhui 233004 );Role of spleen on hepatic regeneration after partial hepatectomy[J];JOURNAL OF BENGBU MEDICINAE COLLEGE;1998-06
5 Zhao Jihong 1) Liang Yu 2) Yan dayu 2) (1)College of Basic Sci., North China Univ. of Tech., 100041, Beijing, China 2) Graduate School of Univ. of Sci. &Tech. of China, 100039, Beijing, China);Structure-Antioxidation Relationship of Flavonoid Antioxidants[J];Journal of North China University of Technology;2001-01
6 XU Bin, HUANG Zhu-qing, HAN Li-hong, et al. (Department of Infectious Diseases, Shanghai Armed Police Hospital, Shanghai 201103, China);The Role of the Replication of Hepatitis B Virus in the Progress of Liver Fibrosis[J];Labeled Immunoassays and Clinical Medicine;2002-03
7 Jiang Jinlan1,Lu Wenfu1,Hu ChunguangXiong Wei1,Yan Weiqun1,Sun Dejun2 (1 Department of Biochemistry,Institute of Biological Engineering,Norman Bethune University of Medical Sciences,Changchun 130021,China; 2 Jilin Workers′ Medical University);Separation and long-term cultivation of rat hepatocytes[J];Journal of Norman Bethune University of Medical Science;2000-06
8 Jin Shugen (The Affiliated Shuguang Hospital of Shanghai University of TCM, Shanghai);Clinical and Experimental Research on Chronic Hepatic Disease Treated with Rougan Kangxian Decoction[J];China Journal of Traditional Chinese Medicine and Pharmacy;1994-04
9 LI Baogang, LI Jiyao, LIANG Huiyun, ZHU Wenyu(Department of Physiology, Beijing Medical University, Beijing 100083)Keywords Taurine/pharmacol Liver cirrhosis, experimental/prev Collagen/metab;THE PROTECTIVE EFFECT OF TAURINE ON RAT HEPATIC FIBROSIS[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1998-04
10 CHEN Xiang-qing,WANG Qin-mao,FANG Hua-wu,et al (Dept.Of Pharmacy,Anhui Provinical Hospital,Anhui Hefei 230001,China);PROTECTIVE EFFECTS OF TOTAL GLUCOSIDES OF PAEONY AND EXTCACT FROM ANGELICA SINENSIS ON EXPERIMENTAL IMMUNOLOGICAL LIVER INJURY[J];Journal of Chengde Medical College;2004-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 QIU Ji1,GUI Shuang-ying2(1.Department of Pharmacy,First Renmin Hospital,Hefei 230061;2.School of Pharmacy,Anhui College of Traditional Chinese Medicine,Hefei 230031);Protective effect of astragalus extract on acute liver injury in mice[J];Anhui Medical and Pharmaceutical Journal;2009-06
2 LI Baowen ZHANG Lan LV ZenghuaDepartment of Surgery,Binzhou Medical College,Binzhou 256603;Effects of astragalus on serum and liver tissue TGF-β1 level in immature rats with obstructive jaundice[J];Journal of Binzhou Medical College;2006-04
3 FU Tingliang1,2 ZHANG Wentong1 GAO Yong2 et al1 Department of Pediatric Surgery,Qilu Hospital of Shandong University,Jinan 250012;2 Binzhou Medical University;Effects of astragalus on liver ultrastructure change and IL-10 mRNA expression in growing rats with obstructive jaundice[J];Journal of Binzhou Medical University;2009-03
4 ZHU Ya-Hong1 YAN Hui1 GUI Zhong-Zheng1,2(1School of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China);A Study on the Protective Effect of Cordyceps militaris Polysaccharide to the Primary Cultured Rat Hepatocytes Injured by CCl_4[J];Science of Sericulture;2009-01
5 WU Guo\|tu\+1, LIN Jian\|zhong\+2, WANG Rui\|xing\+1, HUANG Zi\|qiang\+2 (1.Division of Physiology and Pathophysiology, 2.Division of Pharmacology, Fujian Medical University, Fuzhou\ 350004, China) \ \;The Antitumor Activity of Herb Gui-Lin-San[J];Journal of Fujian Medical University;2003-01
6 WU Guo-tu~1,XU Jian-hua~2,ZUO Wen-zong~3(1.Department of Physiology and Pathophysiology,Fujian Medical University,Fuzhou 350004,China);An Experimental Study of the Chinese Herb"Zhong Zhui Kou Fu Yao Ye" on Antitumor Activity[J];Strait Pharmaceutical Journal;2006-03
7 LI Yan,WANG Hua,ZHOU Xiao-qiong,ZHONG Wen-ping,CHEN Tao-tao(College of Life Science of Longyan University / Key Laboratory of Preventive Veterinary Medicine,Educational Commission of Fujian Province / Fujian Engineering Research Center for Prevention and Control of Zoonosis and Viral Diseases,Longyan 364000,Fujian,China);Effect of Compound Ginkgo Leaf Preparation on Antioxidant Activity and Inhibiting NDV Replication in Vitro[J];Hubei Agricultural Sciences;2010-03
8 CHEN Lei,WU Hao,YAO Jing-qian,CHANG Nian(Nan Jing University of Chinses Medicine,Jiangsu 210029,China);Protective effects of polysaccharides from Hyriopsis cumingii on hepatocytes injured by CCl_4[J];Chinese Journal of Marine Drugs;2008-02
9 SUN Xian-pu,ZHANG Guang-ye,XU Hou-mei,et al.Rizhao traditional Chinese medicine hospital,Rizhao,276800,China.;The clinical study of interventional therapy combining with Jianpi Shugan decoction for treatment of primary hepatic carcinoma with spleen hyperfunction[J];Chinese Imaging Journal of Integrated Traditional and Western Medicine;2007-02
10 XU Jiansheng, YANG Xingyi ,CHEN Dechang, LI Xiujiang,PAN Shuming. Departement of Emergency Medicine,Changzheng Hospital, Shanghai 200003, China;Experimental study on astragalosides injection inhibiting hepatoeyte apoptosis after cardiopulmonary resuscitation in rats[J];Journal of Emergency Medicine;2005-04
China Proceedings of conference Full-text Database 2 Hits
1 ;肝康颗粒对小鼠免疫性肝损伤的保护作用[A];[C];2006
2 Shui Lihua 1,Guo Min 1,Li Chuanxun 2,Yang Tong 2*,Han Guozhu 2,Li Nan 3(1 Department of pharmacy,Dalian Hospital of Dermatology,Liaoning Dalian,116021;2 College of pharmacy,Dalian Medical University,Liaoning Dalian,116044;3 Department of Analytic-Chemical,Dalian University of Sciences,Liaoning Dalian,116000.);Experimental Study of the Tea Polyphenol Cream Enhancing Healing of Skin Ulcer in Rabbit[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved