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《Acta Universitis Medicinalis Nahui》 2005-02
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Deletion, point mutation and promoter methylation of p16 gene in acute lymphocytic leukemia

Liu Xiaoying, Wang Huiyuan, Zhou Qing et al (Laboratory of Molecular Biology, Anhui Medical University, Hefei 230032)  
Objective To investigate the inactivation mechanism of tumor suppressor gene p16 in 29 Chinese acute lymphocytic leukemia(ALL) patients. Methods DNA was extracted from peripheral leukocytes of 29 ALL patients using phenol/chloroform method. The deletions and point mutations of p16 exon2 and methylation variations in promoter CpG islands of p16 by were analysed by PCR(polymerase chain reaction), PCR-SSCP(PCR-single strand conformational polymorphism) and MSP(methylation-specific PCR) methods. Results In these ALL samples, the homozygous deletion rate of p16 exon2 was 27.6%(8/29), no point mutation of p16 exon2 was found; the methylation rate of the promoter CpG islands of p16 gene was 13.8(4/29). Conclusion Inactivation of p16 gene is related to the progression of ALL.
【Fund】: 安徽省自然科学基金 (编号: 99044312);; 安徽省教育厅自然科学基金(编号:JL97 -077 );; 安徽医科大学校科研基金(编号: 522095、9907、9904)资助项目
【CateGory Index】: R733.7
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liu Xiaoyin, Wang Huiyuan, Lu Yunxia et al (Anhui Medical University, Hefei 230032);Establishment and preliminary application of methylation-specific method for detecting p16 gene[J];Acta Universitis Medicinalis Nahui;2002-01
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 YAO Qing-Jun 1,CHEN Bi 1,HU Da-Hai 1,XU Ming-Da 1,DONG Mao-Long 1,WANG Shu-Sen 2 1Department of Burn Surgery, 2Department of Orthopedics,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China;Methylation control of p16 gene expression in early phase of deep second degree burn and hypertrophic scar after burn healing[J];Journal of The Fourth Military Medical University;2005-04
2 Lu Wensheng, Yu Dexin, Zhou Qing et al (Dept of Biochemistry and Laboratory of Molecular Biology, Anhui Medical University, Hefei 230032 );Inactivation of the p16/MTSI gene in bladder cancer[J];Acta Universitis Medicinalis Nahui;2003-04
3 Wang Yasong,Jin Yongtang,Xue Shaoli,et al(School of Public Health, Anhui Medical University, Hefei 230032);Research on methylation of p16 gene in non-small cell lung cancer[J];Acta Universitatis Medicinalis Anhui;2006-06
4 CHEN Wencheng,PAN Shangling,QIN Zhijian,et al Department of Clinical Microbiology and Immunology,Medical Laboratory College,Youjiang Medical College for Nationalities.Baise 533000,China;Relationship of methylation of p16 gene with long life expectancy of Zhuang ethnic group elderly people:report of 156 cases in Guangxi Bama area[J];Chinese Journal of Multiple Organ Diseases in the Elderly;2010-04
China Proceedings of conference Full-text Database 1 Hits
1 ;Effect of Methylation of P16 Gene on Non-small Cell Lung Cancer[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Yingshe,ZHANG Sizhong *.*Department of Medical Genetics, The Affiliated Hospital, West China University of Medical Sciences, Chengdu, 610041 P.R.China.E mail:zszg@wcums.edu.cn;Detection of methylation status of tumor suppressor gene p16 by methylation specific polymerase chain reaction[J];CHINESE JOURNAL OF MEDICAL GENETICS;1999-05
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