The methylation status of TMS1 gene in non-small cell lung cancer and its clinical significance
Wang Conghui,Lu youjin(Dept of Geriatric Pulmonary, The First Affiliated Hospital of Anhui Medical University, Hefei 230022)
Objective To evaluate the methylation status of taget of methylation -induced silencing gene(TMS1)and protein expression of TMS1 in non-small cell lung cancer(NSCLC),and to explore its clinical significance. Methods The methylation -specific PCR(MSP) and the immunohisto-chemistry(IHC)were used to test the methylation status of DNA and the protein expression of TMS1 from 50 lung cancer tissues and 50 corresponding nomalig-nant tissues. Results 20 of 50 non-small cell lung cancer tissues were tested to be methylated,the frequence is40%,and there was no methylation in any corresponding nomalignant tissue(P0.05). The protein expression of TMS1 were decreased in 72% of malignant tissues,but were expressed totally in all corresponding nomalignant tissues,none of them was decreased(P0.05).The methylation status of TMS1 gene was closely correlated with the protein expression of TMS1(P0.05). Conclusion The methylation of promotor region of TMS1 gene is a common event in non-small cell lung cancer,and it is correlated with the down-regulation of protin expression. The methylation of TMS1 gene plays an important role in tumorigenesis of non-small cell lung cancer.
【CateGory Index】： R734.2