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The effect of curcumin on the ultrastructure of kidney and cell proliferation in nephrotoxic sera nephritis rats

BAO Huaying, CHEN Ronghua, GUO Mei, et al. Nangjing Children's Hospital, The Affiliated Hospital of Nanjing Medical University,Nanjing 210008,China  
Objective To clarify whether the curcumin has renal protective role,we scrutinized the display of the pathological and ultrastructure and the expression of proliferation cell nuclear antigen (PCNA) in the kidneys after the nephrotoxic nephritis(NTN)rats received curcumin.Methods 72 healthy male Sprague Dawley rats were divided into three groups of 24 animals each group.Normal control group was injected with normal saline 0 5 ml/day (0 9% NaCl)intra caudal vein for two days,at the same time administered normal saline 0 5 ml/kg intraperitoneally daily;NTN group was injected with 0 5 ml/day nephrotoxic sera through the tail vein for two days and dimethyl sulfoxide (DMSO) 0 5 ml/kg was given intraperitoneally daily;Curcumin group (CUR),was injected with 0 5 ml nephrotoxic sera as above and meanwhile received 50mg/kg/day of curcumin intraperitoneally every day.Animals were killed on days 3,7,14,28 in batch afterwards and samples were collected.Some of renal tissues were fixed in 10% formalin for light microscopy stained with the periodic acid Schiff reaction or eosin,hematoxylin counterstain and for appreciation of the expression of the PCNA by immunohistochemistry or fixed in 4% formaldehydum polymerisatum for electronic micrograph. Results Treatment with curcumin was associated with less inflammatory cell infiltration and tubular interstitial damage,attenuation of the cell proliferation and PCNA positive cell in glomerulus compared with NTN rats.The electronic microscope showed curcumin could relieve the fusion of podocytes foot process and arrest the increased thickness tendency of the glomerular basement membrane.It also suggested curcumin could curb the proliferation of mesangial and epithelium cells and it could diminish the infiltration of neutrophils and monocytes in renal tissue,especially in the interstitial.Conclusion Curcumin relieves the renal injury and ameliorates its ultrastructure change in NTN rats.It might exert its renoprotective role through curbing inflammatory cell infiltrating in renal tissue and inhibiting the mesangial and epithelial cells proliferation.It may have renoprotective role in NTN rats.
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