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《Journal of the Graduates Sun Yat-Sen University(Natural Sciences、Medicine)》 2002-02
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Development of Yeast Two-hybrid System

Zhu Jingyi (Zhongshan University,Guangzhou 510275)  
Yeast two-hybrid system has been the most important means for researching the interaction between proteins since it was constructed.Yeast two-hybrid system was widely used in every biological field,and has revealed the interaction of many unknown protein.On the basic of yeast two-hybrid system,there spring up a lot of new means for its limitation. For instant,the yeast tri-hybrid system,the one-hybrid system,the reverse two-hybrid system,the two-hybrid system in mammal cell,sos system,and so on.Yeast two-hybrid system and the means derive from it has successfully been used to detect the interaction be- tween protei-protein,protein-DNA,protein-RNA,protein-ligand.
【Key Words】: Yeast two-hybrid system One-hybrid system Reverse two-hybrid system Two-hybrid system in mammal cell Sos system
【CateGory Index】: Q503
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