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《Journal of the Graduates Sun Yat-Sen University(Natural Sciences、Medicine)》 2002-02
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Development of Yeast Two-hybrid System

Zhu Jingyi (Zhongshan University,Guangzhou 510275)  
Yeast two-hybrid system has been the most important means for researching the interaction between proteins since it was constructed.Yeast two-hybrid system was widely used in every biological field,and has revealed the interaction of many unknown protein.On the basic of yeast two-hybrid system,there spring up a lot of new means for its limitation. For instant,the yeast tri-hybrid system,the one-hybrid system,the reverse two-hybrid system,the two-hybrid system in mammal cell,sos system,and so on.Yeast two-hybrid system and the means derive from it has successfully been used to detect the interaction be- tween protei-protein,protein-DNA,protein-RNA,protein-ligand.
【Key Words】: Yeast two-hybrid system One-hybrid system Reverse two-hybrid system Two-hybrid system in mammal cell Sos system
【CateGory Index】: Q503
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【References】
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【Citations】
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【Co-citations】
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【Co-references】
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3 LIU\ Fuquan (College of Life Sciences,Peking University,Beijing,100871) ZHANG\ Yanwen (Department of Biology,Nankai University,Tianjin,30071) XU\ Zhihong (Chinese Academy of Sciences,Shanghai Institute of Plant Physiology,Shanghai,200032);Cloning and Primarily Detecting the Function of the Promoter Region of NFL Gene in Tobacco[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;2000-02
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9 HU Cheng xiang 1, XU Xiang 1, LIANG Hua ping 1, LU Feng lin 1, WANG Fu long 2, WANG Zheng guo 3 ( 1Department 1, 3Department 4, Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042; 2Department of General Surgery, No. 285 Hospital of PLA, Handan 056001, China);Design and construction of a random peptide library with yeast two hybrid system[J];Acta Academiae Medicinae Militaris Tertiae;2004-01
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【Secondary References】
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1 Zhang Kaihui(Baoji College of Engineering Technology,Baoji 721013,China);Yeast Two-hybrid System and Application[J];Value Engineering;2011-23
2 AN Hong-xue,SHA Wei,ZHANG Mei-juan,LIU Bo,SONG Lu(College of Life Sciences and Agriculture and Forestry,Qiqihar University,Heilongjiang Qiqihar 161006,China);Cloning, expression vector construction of GpPER1 gene from Grimmia pilifera and its sequence analysis[J];Journal of Qiqihar University(Natural Science Edition);2013-04
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