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《Journal of the Graduates Sun Yat-Sen University(Natural Sciences、Medicine)》 2002-02
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Development of Yeast Two-hybrid System

Zhu Jingyi (Zhongshan University,Guangzhou 510275)  
Yeast two-hybrid system has been the most important means for researching the interaction between proteins since it was constructed.Yeast two-hybrid system was widely used in every biological field,and has revealed the interaction of many unknown protein.On the basic of yeast two-hybrid system,there spring up a lot of new means for its limitation. For instant,the yeast tri-hybrid system,the one-hybrid system,the reverse two-hybrid system,the two-hybrid system in mammal cell,sos system,and so on.Yeast two-hybrid system and the means derive from it has successfully been used to detect the interaction be- tween protei-protein,protein-DNA,protein-RNA,protein-ligand.
【Key Words】: Yeast two-hybrid system One-hybrid system Reverse two-hybrid system Two-hybrid system in mammal cell Sos system
【CateGory Index】: Q503
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【Citations】
Chinese Journal Full-text Database 2 Hits
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【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 LI Xian-kun et al(College of Horticulture and Gardening of Hainan University,Danzhou,Hannan 571737);Research and Application Advances of Yeast Two-hybrid Technique[J];Journal of Anhui Agricultural Sciences;2009-07
2 Cui Hongjun 1 Zhang Junjie 1,2 Huang Yubi 1,3 1 Key Laboratory of South west Crop Genetic Resources and Improvement, Ministry of Education, Maize Research Institute, Sichuan Agricultural University, Yaan, 625014; 2 Life and Physical Science College Sichuan Agricultural University, Yaan, 625014; 3 Agronomy College, Sichuan Agricultural University, Yaan, 625014;Construction and Evaluation of Yeast Two Hybrid cDNA Library of Maize Root[J];Molecular Plant Breeding;2008-01
3 ZHANG Li~(1,2) HUANG Jie~(1*)(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071;2.LaiYang agriculture college,Department of plant protection,Qingdao 266109);THE IMPROVEMENT AND ADVANCE IN RESEARCH OF YEAST TWO-HYBRID SYSTEM[J];Transactions of Oceanology and Limnology;2006-01
4 ZHU Hao ZHU Hui YANG Qi CHEN Chun-fen ZHANG Zhong-ming(College of Life Science and Technology/State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China);Construction of Lotus japonicus AD-cDNA Library and Identification of Proteins Interact with NFR1[J];Journal of Huazhong Agricultural University;2007-06
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6 LIN Cun-gang~1,2,XU Hui~1;CHEN Yu-cheng~2 (1.Inst.of Applied Ecol.Chinese Acad.of Sci.Shenyang 110016;2.Coll.of Resources & Environm.SW Agric.Univ.Chongqing 400716);Advance in Research and Application of Yeast Double-Hybrid System[J];Journal of Microbiology;2005-06
7 Chen Guisheng1,Shi Shugui1,Li Lusi1,Chen Kangning1,Yuan Shunzong2,Peng Xu2,Wu Jun2,Luo Gaoxing2(1.Department of Neurology,the Affiliated Southwest Hospital of Third Military Medical University,Chongqing,China 400038;2.Research Institute of Burn,the Affiliated Southwest Hospital of Third Military Medical University,Chongqing,China 400038);Subcloning Mutant SOD1cDNA into pGBKT7 of Yeast Expression Plasmid[J];China Pharmaceuticals;2008-01
8 WUYUN Ta-na, ZHANG Dang-quan, TAN Xiao-feng (The Key Lab. of Non-wood Forest Product of Forestry Ministry, CSFU, Changsha 410004, Hunan, China);Progress in Proteomics Technologies and Its Application to Plant Research[J];Journal of Central South Forestry University;2005-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HU Cheng xiang 1, XU Xiang 1, LIANG Hua ping 1, LU Feng lin 1, WANG Fu long 2, WANG Zheng guo 3 ( 1Department 1, 3Department 4, Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042; 2Department of General Surgery, No. 285 Hospital of PLA, Handan 056001, China);Design and construction of a random peptide library with yeast two hybrid system[J];Acta Academiae Medicinae Militaris Tertiae;2004-01
2 HU Chengxiang,XU Xiang,LIANG Huaping,WANG Fulong,LUO Yan,WANG Zhengguo(Research Institute of Surgery, Third Military Medical University, Chongqing,Sichuan,400042,China);Design and Construction of a Random Peptide Library with Yeast Two-Hybrid System[J];Progress In Veterinary Medicine;2003-02
3 WEI Taiyun, LIN Hanxin, XIE Lianhui*(Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Application of yeast two-hybrid system to plant virology[J];;2003-01
4 XIA Xin-ming,LIU Jin-xian,LIN Ming,WANG Yu-na,HE Shui-lin(College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);The construction of cDNA library with RNA isolated from young fruit of grape challenged with ultraviolet radiation[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2007-02
5 HUANG Gong-hua,LIANG Jing-yao,LIU Xin-guang,LIANG Nian-ci (Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang 524023 China);Construction of yeast two-hybrid cDNA library of HL-60 cells in yeast cells[J];Journal of Guangdong Medical College;2004-01
6 WANG Zhi-cheng,JIANG Jian-xiong*,ZHONG Jun,YI Zi-li,LIU Qing-bo (Key Laboratory of Cell Engineering,HNAU,Changsha 410128,China);Rapid Isolation of Full-Length cDNA Sequence from Gossypium hirsutum Fiber cDNA Library by PCR[J];Journal of Hunan Agricultural University;2005-03
7 ZHANG Li~(1,2) HUANG Jie~(1*)(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071;2.LaiYang agriculture college,Department of plant protection,Qingdao 266109);THE IMPROVEMENT AND ADVANCE IN RESEARCH OF YEAST TWO-HYBRID SYSTEM[J];Transactions of Oceanology and Limnology;2006-01
8 WANG Yu-hui,HU Li-juan,LU Fang,LIU Yan-you,JI Zhi-hong,JIANG Zhou,YANG Bo,WANG Zheng-rongSchool of Basic Medicine and Forensic Medincine,West China Medical Center,Sichuan University,Chengdu Sichuan 610041,China;Research on Protein Interacting with hPeriod1.[J];Space Medicine & Medical Engineering;2006-04
9 ZHU Jing~1,YANG Na-Na~2,LI Hao~1,DU Li-Xin~(3*)(1.Shandong Provincival Center of Laboratory Animals,Jinan,250002 China;2.Taishan Medical University,Tai'an,271016,China;3.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094,China);A Research on the Transformation from λ Phage Library to Plasmid Library[J];Acta Ecologiae Animalis Domastici;2006-01
10 SHI Ming-Wang~(1,2), TAN Xiao-Feng~1, WANG Yi-Qiang~1, HU Fang-Ming~1 ional Forestry Ministry, CSFU, Zhuzhou 412006, Hunan, China; 2.Science and Technology Institute of Henan, Xinxiang 453002, Henan, China);Question and Solution of Construction cDNA Library from Seed of Camellia oleifera[J];Economic Forest Researches;2004-02
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