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《Chinese Ophthalmic Research》 2001-05
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Differentiation of fungal species in fungal keratitis by confocal microscopy

Li Shaowei, Gebhardt BM, Shi Weiyun, et al. Shandong Eye Institute, Qingdao 266071  
Objective To evaluate the possibility of distinguishing among three fungal species using confocal microscope in the living eye. Mathods 18 rabbits were divided into three groups. Their right Corneas were inoculated with three different fun- gal species. The central corneal epithelium was removed within a 5 mm diameter and an aliquot of 1 × l07 cell/ml of Candida albican suspension was instilled or some cultured spores of Aspergillus funigatus or Fusarium solani were placed on the corneal surface. A soft contact lens was used to cover the cornea and tarsorrhaphy was performed. The infected corneas were examined by confocal mi- croscopy, smears and histopathological analysis at 5, 10, and 15 days postinoculation. Results In 1 - 5 days after infection,fungi- and acute inflammatory cells be seen in the anterior stroma. During 5-10 days,the host response suppressed fungal growth;by days 10-15,the infections were resolved. However, the diseased procession and the inflammatory reaction were severe in the Aspergillus and Fusarium than Candida infection. Budding yeast predominated after Candida infection and appeared as bright,hyper-reflective structures. Later,it became chain-like structures. Hyphae from Aspergillus appeared as slightly thicker, hyper-reflective branching structure compared with Candida. After mid stage,it became longer and less branch structures and stayed a longer time in corneal tissue than Candida. Hyphae of Fusarium appeared as worm-shape structures at the cornea surface at the early stage,became thicker stick-like structures with fewer branches. These results correlated with inspection of the smears and the histopathology study. Con- clusion The confocal microscopy provides valuable information in differentiation of fungal species in the living eye.
【CateGory Index】: R772.21
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【References】
Chinese Journal Full-text Database 5 Hits
1 Xue-Yong Wang,Xu-Mei Wang Department of Ophthalmology,Red Cross Light Hospital of Junan County,Junan County 276600,Shandong Province,China;Application of confocal microscopy in fungal keratitis[J];国际眼科杂志;2011-02
2 Zhang Jun,Wang Liya,Sun Shengtao,Zhang Yueqin.Department of Ophthalmology,Affilliated First Hospital of Zhengzhou University,Zhengzhou 450002.Henan Eye Institute of Ophthalmology,Zhengzhou 450003,China;Clinical features and confocal microscopic imaging characteristics of Fusarium keratitis[J];眼科研究;2008-03
3 SHI Wei-yun ,NIU Xiao-guang,WANG Fu-hua, GAO Hua, LI Shao-wei, ZENG Qing-yan, XIE Li-xin. Shandong Eye Institute and Qingdao Eye Hospital, Qingdao 266071,China;Evaluation of antifungal chemotherapeutic effects on fungal keratitis by confocal microscopy[J];中华眼科杂志;2005-07
4 XIAO Cai wen,MA Jing xue,XU Shen,FAN Yu xiang,HOU Si qing,YANG Yun dong,CUI Hui lai,CHEN Jun e. 1The Central Hospital of Cangzhou; 2The Secord Hospital of Hebei Medical Science Univeristy 061001 China;The experimental study of keratomycosis treated by Ozone solution[J];中国实用眼科杂志;2004-12
5 Li Shaowei,Fang Jing, Gebhardt BM,et al. Shandong Eye Institute and Hospital, Qingdao 266071;A comparison of virus isolation and PCR with antigen membrane test in diagnosis of HSK[J];眼科研究;2002-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 XIE Lixin, LI Shaowei, SHI Weiyun, et al. Institute of Ophthalmology, Shandong Academy of Medical Sciences, Qingdao 266071;Clinical diagnosis of fungal keratitis by confocal microscopy[J];中华眼科杂志;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Cheng Jun;Zhai Hualei;Wang Junyi;Xie Lixin;Qingdao Eye Hospital, Shandong Eye Institute;;Clinical features and treatments of retrocorneal fungal infection[J];中华眼科杂志;2017-10
2 YANG Jie;NIU Ji-ming;DU Min;The Second People’s Hospital of Zhengzhou City;;Observation and Analysis of Fungal Hyphae Form under Confocal Microscope[J];中国医学创新;2015-36
3 WANG Guang-xue;Liangqiu Community Hospital of Feixian County;;CLINICAL ANALYSIS OF KERATOMYCOSIS[J];山东医学高等专科学校学报;2015-02
4 CUI Feng-hua;LI Suxia;SHI Weiyun;Shandong Academy of Medical Sciences,Jinan University;Shandong Eye Hospital,Shandong Eye Institute;;The retrospective analysis of causes of misdiagnosis and the prognosis of bacterial corneal ulcer[J];临床眼科杂志;2015-01
5 Zheng Yongzheng;Xie Maosong;Liu Guanghui;Ren Bingyi;Pan Mingdong;Department of Ophthalmology,The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine;Department of Ophthalmology,The First Affiliated Hospital of Fujian Medical University;;Application of 2% mercurochrome solution staining in the diagnosis of fungal keratitis[J];中国实用眼科杂志;2014-12
6 Pei Sen;Ji Yazhou;Wu Aihong;Shen Lei;Mu Zechen;Anyang Eye Hospital;;Observation of the effects that fungal keratitis treated by corneal lamellar resection combined with amniotic membrane(AM) transplantation using confocal microscopy[J];中国实用眼科杂志;2014-09
7 LIU Su-yuan;ZHANG An-ping;SHAN Xiu-shui;YUE Li;Eye Hospital in Tangshan City;Life Sciences,Hebei Union University;;Pathogenic analysis and diagnosis of dungal keratitis inTangshan Region[J];中国实用眼科杂志;2013-07
8 Ang Li,Zhong-Yi Fan Department of Ophthalmology,No.463 Hospital of Chinese PLA,Shenyang 110042,Liaoning Province,China;Clinical study of confocal laser microscopy in fungal keratitis[J];国际眼科杂志;2013-06
9 Yan-Dong Liu Xingtai Eye Hospital, Xingtai 054001, Hebei Province, China;Conjunctival flap covering combined with iris incarcerating surgery for the treatment of full-thickness fungal keratitis[J];国际眼科杂志;2012-05
10 YAO Xiao-ming,CAO Duan-rong,LI Ming,NIE Dan-yao,YU Li.Shenzhen Eye Hospital,Ji-nan University, Shenzhen 518000,China;Comparative study of the laser scans confocal microscopy examination of fungal corneal ulcer before and after the corneal scraping[J];中国实用眼科杂志;2011-11
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yang Zheng, Yi-Ping Qiao, Xi-Bo Zhang, Guang-Rong Liao, Zhao-Xia Huang Eye Center, the Affiliated Hospital of Luzhou Medical College,Luzhou 646000, Sichuan Province, China;Clinical analysis of early diagnosis of 70 cases of fungal keratitis[J];国际眼科杂志;2010-04
2 Lei Jin1,Jian-Ping Cui21Xuzhou Eye Hospital,Xuzhou 221002,Jiangsu Province,China; 2Department of Ophthalmology,Xuzhou First People's Hospital,Xuzhou 221002,Jiangsu Province,China;Clinical application of corneal confocal microscopy in the etiology of corneal ulcer diagnosis[J];国际眼科杂志;2008-12
3 SHI Wei-yun ,NIU Xiao-guang,WANG Fu-hua, GAO Hua, LI Shao-wei, ZENG Qing-yan, XIE Li-xin. Shandong Eye Institute and Qingdao Eye Hospital, Qingdao 266071,China;Evaluation of antifungal chemotherapeutic effects on fungal keratitis by confocal microscopy[J];中华眼科杂志;2005-07
4 PAN Fei,ZHANG Bei,YAO Yu-feng,QIU Wen-ya. Department of Ophthalmology,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China;Clinical diagnosis of fungal keratitis using confocal microscopy[J];中国实用眼科杂志;2004-01
5 ZHANG Wenhua, PAN Zhiqiang, WANG Zhiqun, JIN Xiuying, LUO Shiyun, ZOU Yang, WU Yuying, LI Ran. Beijing Institute of Ophthalmology, Beijing 100005, China Corresponding author:ZHANG Wenhua (Email: panxiaow@public3.bta.net.cn);The variance of pathogenic organisms of purulent ulcerative keratitis[J];中华眼科杂志;2002-01
6 Li Shaowei, Gebhardt BM, Shi Weiyun, et al. Shandong Eye Institute, Qingdao 266071;Differentiation of fungal species in fungal keratitis by confocal microscopy[J];眼科研究;2001-05
7 SUN Dong Ping 1 LU Ping 1 ZHANG Ai Juan 1 FANG Zhi Jie 2 LI Hong Zhao 2 (The chemical school of NUST,NanJing 210094) 1 (The life institute of NanJing University,NanJing 210093) 2;STUDY ON THE STERILIZATION EFFECTS OF FUNGI BY THE TREATMENT OF OZONE[J];微生物学通报;2001-01
8 WANG Liya(Email:liyawang1@yahoo.com), ZHANG Yueqin, WANG Yinqi, et al Henan Institute of Ophthalmology, Zhengzhou 450003,China;Spectrum of mycotic keratitis in China[J];中华眼科杂志;2000-02
9 XIE Lixin, LI Shaowei, SHI Weiyun, et al. Institute of Ophthalmology, Shandong Academy of Medical Sciences, Qingdao 266071;Clinical diagnosis of fungal keratitis by confocal microscopy[J];中华眼科杂志;1999-01
10 Zhang Wenfu, et al Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071;TEST FOR EFFICACY OF OZONE SOLUTION IN ACTIVATING VESICULAR STOMATITIS VIRUS[J];中国消毒学杂志;1998-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Wang Liya;Xu Zhongzhong;Zhang Junjie;Sun Shengtao;Li Jin;Yu Xiaofei;Zhu Lei;Zhang Yueqin;He Yi;Li Jiachen;Wang Lulu;Tao Siyu;Henan Eye Institute,Henan Eye Hospital,People's Hospital of Zhengzhou University;;Topical voriconazole as an effective treatment for fungal keratitis[J];中华眼科杂志;2016-09
2 Xia Yuan;Xue Chunyan;Wu Yan;Huang Zhenping;Department of Ophthalmology,Nanjing General Hospital of Nanjing Military Command PLA;;The features of in vivo laser confocal microscope images of frequent fungal keratitis[J];中华实验眼科杂志;2016-02
3 YANG Jie;NIU Ji-ming;DU Min;The Second People’s Hospital of Zhengzhou City;;Observation and Analysis of Fungal Hyphae Form under Confocal Microscope[J];中国医学创新;2015-36
4 Ma Junxin;Wang Linnong;Zhou Ruxia;Yu Yang;Du Tongxin;Department of Ophthalmology,Nanjing First Hospital,Nanjing Medical University;;Application of real-time PCR in pathogenic detection of necrotizing herpes stromal keratitis[J];中华实验眼科杂志;2015-05
5 Qiong Xie;Ming-Hong Gao;Hai Yu;Department of Ophthalmology,the General Hospital of Shenyang Military Command;;Clinical observation of cryotherapy and amniotic membrane transplantation in the management of Fusarium corneal ulcer[J];国际眼科杂志;2014-10
6 Hui Xiao;De Yan Zhang;Zhong Yi Fan;Department of Ophthalmology,the No.463 Hospital of Chinese PLA;;Clinical features and confocal microscopic imaging characteristics of 466 cases with infectious keratitis[J];国际眼科杂志;2014-10
7 Shi Weiyun;Xie Lixin;Shandong Eye Hospital,Shandong Eye Institute;;The status quo and expectation of corneal research in China[J];中华眼科杂志;2014-09
8 Pei Sen;Ji Yazhou;Wu Aihong;Shen Lei;Mu Zechen;Anyang Eye Hospital;;Observation of the effects that fungal keratitis treated by corneal lamellar resection combined with amniotic membrane(AM) transplantation using confocal microscopy[J];中国实用眼科杂志;2014-09
9 LIU Changxiu;OU Lanzhi;ZHOU Xiaoyan;Lanshi Hospital of Chancheng District;The Third People's Hospital of Nanhai District;Zhujiang Hospital of Southern Medical University;;Clinical Observation on LEEP Electro-Scalpel Combined with Ozone in the Treatment of Bartholin Gland Cyst[J];临床医学工程;2014-05
10 ZHAO Zhi-wei;ZHANG Yun-li;The Central Hospital of Jinzhou City;;Analysis of Laboratory Rapid Diagnostic Method of Fungal Keratitis[J];中国医学创新;2014-01
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