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Effects of gypenosides on platelet aggregation release and cAMP level in rabbits

WU Ji-Liang,QIU Pei-Lun,LIU Jun-Tian,MU Qi-Yun,XIN Dong-Sheng (Dept Pharmacology,Xian Medical University,Xian 710061)  
The antiplatclet aggregation effect of gypenosides(GPs)extracted from Gynoslemma pentaphyllun(Thnb.)Mak.was studied in platelet-rich plasma(PRP)of the rabbit by Bern's turbidometry.GPs inhibited platelet aggregation induced by threshold concentration of ADP,arachidonic acid and collagen in a dose-dependent manner in vitro and IC50 was about 0.49,0.47 and 0.50mg/ml,respectively.GPs(40 mg/kg)also inhibited platelet aggregation in vivo,approximately lasting for 60 min and its maximal inhibition rate for ADP,arachidonic acid and collagen was 32.6,39.6 and 35.7%,respectively.The most powerful action occured at 5 min after giving GPs.In order to study its antiplatelet mechanism,effects of GPs on platelet serotonin release and cAMP level were further studied in PRP of therabbit by fluorescence spcctrophotomctry and in washed platelet suspension of the rabbit by radioimmunoassay,respectively.GPs and aspirin both inhibited platelet serotonin release induced by collagen with inhibition rate of 87.1 and 91.7% at 1.00 mg/ml,respectively.GPs also significantly increased the platelet cAMP level,with increasing rate of 200% at 1.00 mg/ml.The results suggest that GPs is a potent inhibitor to platelet aggregation and its action mechanism may related to inhibiting the platelet from releasing reaction and increasing the platelet cAMP level
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