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《Chinese Journal of Pharmacology and Toxicology》 2007-03
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Preventive effect of asiaticoside on lipopolysaccharides-induced fever and its influence on levels of inflammatory factors in rats

ZHANG Zhuo1,WAN Jing-Yuan1,LI Hong-Zhong1,LUO Fu-Ling1,ZHOU Qi-Xin1,ZHANG Li2(1.Department of Pharmacology,Pharmaceutical Science College,Chongqing University of Medical Sciences,Chonqing 400016,China;2.Department of Pathophysiology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)  
AIM To explore the pyretolytic effect of asiaticoside.METHODS Fifty-six male SD rats were randomly divided into normal,model,vehicle,paracetamol and 3 doses of asiaticoside groups.Rat fever model was made by ip lipopolysaccharides(LPS,100 μg·kg-1).The changes of the core temperature(ΔT)were tested every 30 min after ip LPS,and lasted for 6 h.The rats in asiaticoside(5,15 and 45 mg·kg-1)groups were ig asiaticoside for 3 d before ip LPS.The rats of normal and model groups were ig normal saline,and rats of vehicle group were ig 0.5% carboxymethyl cellulose,instead of asiaticoside.The rats of paracetamol group were ip paracetamol(50 mg·kg-1)only once and 30 min later ip LPS.The myeloperoxidase(MPO)activity in liver was detected with the MPO kit.The protein expressions of cyclooxygenase-2(COX-2)in brain tissue and heme oxygenase-1(HO-1)in liver tissue were analyzed with Western-blot.The prostaglandin E2(PGE2)content in plasma was detected with enzyme immunoassay and the activity of HO-1 in liver tissue was detected with Morita method.RESULTS The fever appearance of the model rats had three febrile phases,and the maximum amplitude of temperature achieved 2.01℃.Compared with model group,the core temperatures of rats in asiaticoside(5,15 and 45 mg·kg-1)groups were obviously lower,and asiaticoside 45 mg·kg-1 group was similar to paracetamol group.Asiaticoside(5,15 and 45 mg·kg-1)obviously reduced the protein expression of COX-2 in brain,MPO activity in liver and PGE2 content in plasma.The activity and expression of HO-1 in liver were elevated after ig asiaticoside(5,15 and 45 mg·kg-1).CONCLUSION Asiaticoside has the inhibitory effect on fever,and this action possibly is related to its inhibition of the COX-2/PGE2 system,reduction of MPO activity and enhancement of the activity and expression of HO-1.
【Fund】: 国家自然科学基金资助项目(30500463)~~
【CateGory Index】: R285.5
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