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Malignant transformation of Syrian hamster embryo cell by cyclophosphamide

DAI XiangGuo, WU DeChang1, WANG ZhiQiao(Institute of Pharmacology and Toxicology, 1 Institute of Radiation Medicine of Academy of Military Medical Sciences, Beijing 100850)  
The morphological transformation occurred steadily in Syrian hamster embryo(SHE) cell treated with cyclophosphamide(CP) at 37.5, 75, and 150 mg·L1respectively. There was a weak correlation between dose and the rate of transformation. The biological characteristics of two cell strains derived from different batch of experiments had identified. The results showed that the two cell strains still had strong division and proliferation ability at the 65 th and 99 th generation respectively and they could also grow well in low serum medium and greatly decreased the dependence upon fibroblast growth factor. It was also found that agglutination happened when the both of two cell strains were treated with phytoagglutinin(PHA). Furthermore, it was observed that the two cell strains could form cloning in soft agar(anchorage independent growth) and tumors in the body of the homogeneous species and athymic nude mouse. According to pathological study, the tumors were highdifferentiated fibroblast sarcoma.
【CateGory Index】: R979.1
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