Effects of nerve growth factor on intracellular free Ca2+in isolated brain cells of neonatal rat
Wu Junfang Zhang Juntian(Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050)
Aim Effects of nerve growth factor (NGF) on intracellular free Ca2+ concentration (i) in isolated brain cells of neonatal rat were observed. Methods i was measured with Ca2+ sensitive fluorescent indicator, Fura-2/AM, in isolated brain cells of neonatal rat, and the effects of nerve growth factor (NGF) on the changes in i induced by glutamate (Glu) and hydrogen perioxide (H2O2) were studied. Results The resting i was 208 ±22 nmol/L when the brain cells were incubated in Hanks' solution containing Ca2+ 1.3nmol/L. NGF did not alter the resting i in brain cells. NGF 1～100μg/L dose-dependently attenuated glutamate (0.5 mmol/L) and hydrogen peroxide (0.05 mmol/L) induced elevation of i by 10%, 18%, 32%, 44%, 62% and 14%, 30%, 39%, 49%, and 65%, respectively, with an IC50 of 42.5 and 27.3μg/L (95% confidence limits:28.1～71.5 and 18.1～46.5 μg/L). Conclusion These suggest that NGF protect cortical neurons against glutamate toxicity and oxidative insults via reducing the elevation of i or stabilizing i level.
【CateGory Index】： R965