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《Chinese Journal of Clinical Pharmacology and Therapeutics》 2006-11
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Effect of triptolide on proliferation and apoptosis in A_ 549 lung cancer cell

SONG Lan, XU Zhao-jun~1, ZHANG Cai-ping, QIAO Xin-hui Department of Molecular Biology and Biochemistry, Nanhua University, Hengyang 421001, Hunan, China;~1Cardiothoracic Surgery of the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan, China  
AIM: To study the effect of triptolide on cell proliferation and apoptosis in A_ 549 human lung cancer cell. METHODS: The effect of triptolide on cell viability in A_ 549 cell was evaluated by MTT assay. Cell apoptosis was observed by flow cytometry and DNA ladder analysis. Activities of caspase-9 and caspase-3 were assayed with caspase colorimetric assay kit. RESULTS: Triptolide (eg., 0.5 - 4.0 μg·ml~ -1 ) inhibited cell growth and induced apoptosis in a dose-dependent way at 24 h in A_ 549 cells. The activation of caspase-9 and caspase-3 was significantly increased at 24 h in A_ 549 cells when exposed to triptolide (eg., 0.5 - 4.0 μg·ml~ -1 ). CONCLUSION: Triptolide induces apoptosis in A_ 549 cells partly through mitochondrial signal transduction pathway.
【Fund】: 湖南省科技厅基金项目(№05SK3084);; 衡阳市科委项目(№2005SK01-017)
【CateGory Index】: R734.2
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