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《Journal of Yunnan University(Natural Sciences Edition)》 2009-03
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Purification of β-endoglucanase in T. reesei QM9414 and its enzymological study

WU Hong1,NONG Xiang2,ZHANG Qi1,QIN Tian-ying1,HUANG Wei-wei3,TAN De-yong3(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China;2.College of Chemistry and Life Science,Leshan Normal University,Leshan 614000,China;3.School of Life Science,Yunnan University,Kunming 650091,China)  
Two active components Eg1 and Eg2 of β-endoglucanase were purified by utilizing salting out,Sephadex G-75 chromatography and DEAE-Sephadex A50 chromatography from the fermentation broth of Trichoderma reesei QM9414.The relative molecular weights of Eg1 and Eg2 were 65.47ku and 57.04ku respectively;The optimal temperatures of Eg1 and Eg2 were 50℃ and 55℃ respectively;the optimal pH was 4.8 and 5.0 and their Michaelis constants were Km=3.76×10-2g/L and Km=4.20×10-2g/L.According to their enzymological characters,they are newly discovered.It seemed that the research built a basis for the further study of β-endoglucanase in T.reesei QM9414.
【Fund】: 西南民族大学自然科学基金项目资助(23444)
【CateGory Index】: Q78
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