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《Journal of Yunnan University(Natural Sciences Edition)》 2009-03
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Purification of β-endoglucanase in T. reesei QM9414 and its enzymological study

WU Hong1,NONG Xiang2,ZHANG Qi1,QIN Tian-ying1,HUANG Wei-wei3,TAN De-yong3(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China;2.College of Chemistry and Life Science,Leshan Normal University,Leshan 614000,China;3.School of Life Science,Yunnan University,Kunming 650091,China)  
Two active components Eg1 and Eg2 of β-endoglucanase were purified by utilizing salting out,Sephadex G-75 chromatography and DEAE-Sephadex A50 chromatography from the fermentation broth of Trichoderma reesei QM9414.The relative molecular weights of Eg1 and Eg2 were 65.47ku and 57.04ku respectively;The optimal temperatures of Eg1 and Eg2 were 50℃ and 55℃ respectively;the optimal pH was 4.8 and 5.0 and their Michaelis constants were Km=3.76×10-2g/L and Km=4.20×10-2g/L.According to their enzymological characters,they are newly discovered.It seemed that the research built a basis for the further study of β-endoglucanase in T.reesei QM9414.
【Fund】: 西南民族大学自然科学基金项目资助(23444)
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 SONG Jin-zhu,YANG Qian,CHEN Zhong-xiang,ZHANG Jing(Dept.of life science and bioengineering,Harbin Institute of Technology,Harbin 150001,China,);Purification and characteristics of celluloses from trichoderma aureoviride[J];Journal of Harbin Institute of Technology;2006-11
2 CHEN Hong-Ge; ZHU Jing; LIANG Gai-QinYAN Zi-Zheng;JIA Xin-Cheng; ZHANG Shu-Zheng(Institute of Microbiology, Academia Sinica,Beijing 100080);PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER[J];MYCOSYSTEMA;2000-01
3 Cao Jian;Study on Purification and Characteristics of Trichoderma reesei Cellulase[J];Food Science;2003-05
4 WU Hong1,2,QIN Tian-ying1,TAN De-yong2,NONG Xiang3(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China;2.School of Life Sciences;Yunnan University;Kunming 650091 China;3.College of Chemistry and Life Science,Leshan Normal University,Leshan 614000,China);Study on production of cellulase using bagasse by Trichoderma reesei QM9414[J];Journal of Yunnan University(Natural Sciences Edition);2008-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YE Sheng-mei,WANG Lan-lan,OUYANG Ming (Dept. of Bioch. Engn., Anhui University of Technology and Science, Wuhu 24100 0,China);Preliminary studies of solid-state fermentation by using fungi to produce cellulase[J];Journal of Anhui Institute of Mechanical and Electrical Engineering;2003-01
2 NIE Guang-jun,XUE Zheng-lian,YUE Wen-jin,XIAO Bi-qing,CHEN Jun(Dept.of Bioch.& Engn.,Anhui University of Technology and Science,Wuhu 241000,China);Study on α-amylase excreted by bacillus subtilis acted with different content of colchicine[J];Journal of Anhui University of Technology and Science(Natural Science);2006-03
3 CHEN A-na,TANG Bin,ZHANG Qing-qing,ZHAI Guang-wen,ZHOU Feng-yun (Dept.of Bioch.Engn.,Anhui University of Technology and Science,Wuhu 241000, China);Fermentation conditions for cellulase production by Rhizopus sp. TC1[J];Journal of Anhui University of Technology and Science(Natural Science);2006-04
4 Tang Bin Zhang Qinqing (Anhui Institute of Mechanical and Electrical Engineering Wuhu 241000);Study of Lipidase Production with Liquid Culture in Monascus Anka[J];JOURNAL OF ANHUI INSTITUTE OF MECHANICAL AND ELECTRICAL ENGINEERING;1997-04
5 Liu Bin et al (Department of Biotechnological and Sciences Hefei University, Hefei 230022);Study on Liquid Fermentation of Rhizopus delemar Lipase and Enzyme Properties[J];Journal of Anhui Agricultural Sciences;2003-06
6 ZHANG Hong et al (Department of Chemical and Biological Engineering of Yancheng Institute of Technology, Yancheng, Jiangsu 224003);Effect of Culture Condition on the Kinds and Activities of Degrading Enzyme of Cell Wall[J];Journal of Anhui Agricultural Sciences;2005-07
7 ZHANG Su-jiang et al (Institute of Animal Science and Technology,Tarim University,Alar,Xinjiang 843300);Effect of Cellulase on Cotton Straw Fermentation[J];Journal of Anhui Agricultural Sciences;2007-04
8 GU Song et al (Biotechnology Research Institute,CAAS,Beijing 100081);Research Progress on Cellulase[J];Journal of Anhui Agricultural Sciences;2007-25
9 LIN Yuan-shan et al (Department of Bioengineering,College of Bioscience & Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128);Screening and Preliminary Identification of a Nuclease Producing Strain[J];Journal of Anhui Agricultural Sciences;2007-28
10 YAO Fu-rong et al (College of Chemistry and Environmental Science,Guizhou University for Nationalities,Guiyang,Guizhou 550025);Study on the Extraction of Lysozyme from Eggshell[J];Journal of Anhui Agricultural Sciences;2007-34
China Proceedings of conference Full-text Database 1 Hits
1 Jiang Zheng-yu1,Zhou Yan-min,Wang Tian (College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095);Advances of Studies on Activity Change Pattern of Endogenous Digestive Enzyme in Mono-gastric Animals[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lin Qinghua Li Changjian Li Yan (School of Life Science, Wuhan University Wuhan 430072);Preliminary Study on the Production of Singlecell Protein from Bagasse[J];AMINO ACIDS & BIOTIC RESOURCES;1998-01
2 DAI Si\|fa\ \ HE\ Yan\|cai 2 (1.Department of Animal Science,Anhui Technical Teacher College,Fengyang 233100; 2.College of Chemical Engineering.Huaqiao University,Quanzhou 362011);Study on Secretory Characteristics and Enzymolysis Condition of Cellulase System from Tvichoderma Vivide[J];Journal of Anhui Agrotechnical;2001-04
3 Sun Junshe; Li Xue; Dong Xiuqin. College of Food Science, China Argri. Univ.,100083, P.R.China.;Induced breeding and conditions of producing cellulase by Trichoderma koningii.[J];Journal of Beijing Forestry University;2002-02
4 Wu ping,Li Zhengpeng(college of life science,Anhui Science and Technology University,Fengyang Anhui 233100);Study of the screening of the superior strain cell for the production of single cell protein by sugar cane residues[J];Forest By-Product and Speciality in China;2007-04
5 Wang Weiyun Zhu Jinhua Wu Shouyi;Research Progress of Cellulose Science and Cellulase[J];JOURNAL OF JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY;1998-03
6 Nie Yanli1,2 Liu Yongguo3 Li Ya1 Zhao Yonghong4 Li Yuan2 (1.Academy of Yunnan Forestry, Kunming, 650204,2.Yunnan Agricultural University, Kunming, 650201, 3.Wenshan Institute of Forestry Science in Yunnan,Wenshan 663000 4. Academy of Yunnan Tropical Forestry, XSBN,666102);Report on Utilization and Prospect of Sugar Cane Bagasse Resources[J];Forestry Economics;2007-05
7 PanFeng YangShulin ShiXiaoli BaoXiaofeng (School of Chemical Engineering,NUST,Nanjing 210094);Purification and Properties of Cellulose Activity from A.usamii[J];Journal of Nanjing University of Science and Technology;2001-04
8 Du Zongjun 1 Chen Guanjun 2 Gao Peiji 2 Xu Huaishu 1(1 College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003,China)(2 College of Life Sciences, Shandong University, Jinan 250100, China);Purification and Properties of Cellulases from Stachybotrys Atra[J];Journal of Ocean University of Qingdao;2002-01
9 JIANG Jun-Ping; YAN Zi-Zheng and ZHANG Shu-Zheng(Institute of Microbilogy, Academia Sinica, Beijing100080);The Mode of Action of Xylanase from Aspergillus phoenicis on Xylooligosaccharides[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1995-03
10 Yang Shengyuan;Study on β - glucodydase Production Conditions of Aspergillus niger(As. n. XD - 1)[J];Food Science;2002-11
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