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《Journal of West China Forestry Science》 2015-04
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Callus Induction of Taxus wallichianga Zucc.

ZHUO Ga;YANG Xiao-lin;XIN Fu-mei;ZHA Sang;CHEN Xiang-zhen;Farming and Husbandry College,Tibet University;  
Using young leaf,stems and root of Taxus wallichianga Zucc. as material,the effects of different explants,basic medium, plant growth regulators, antioxidants, and culture conditions on callus induction were studied. The results showed that the callus induction rate of young leaves was higher than that of the young stems and roots and the callus volume was bigger than that of others. The basic medium of MS,1 /2 MS,B5 and WPM all could produce callus induction. Among them,the induction effect of MS medium was the best,significantly higher than other basic medium. When adding 1 mg / L 6-BA and 0. 5 mg / L 2,4-D on MS medium,induction rate reached 93. 6 %,and the callus growth is most productive. When adding 1 mg / L Vc to MS basal medium,the browning phenomenon has been greatly inhibited and callus growth was better. Dark culture conditions is good for callus induction and it could induct qualified callus.
【Fund】: 国家自然科学基金项目(31330015);; 国家科技支撑项目(2013BAC04B01)
【CateGory Index】: S791.49
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