TISSUE CULTURE OF EPIPREMNUM AUREUM LEAF BLADES IN VITRO
Lin Dehui (Kunming Institute of Botany, Academia Sinica, Kunming)
The excised segments of leaf blade of Epipremnum aureum (Linden et Andre) Bunting have been cultured on MS ( Murashige and Skoog ) medium supplemented with 6 -BA, IAA, NAA, 2,4-D and 10% coconut milk. With the enlargement of leaf area of excised explants the margin of leaf explants gradually produced some calli. When these explants having the calli are transplanted to SH (Schenk and Hildebrandt) medium supplemented with 6 -BA, the calli on the explants produce small, white and spherical outgrows, and the apices of outgrows appear green colour, and then the green outgrows gradually differentiate into young buds. When young buds are excised from the explants and transplanted to the fresh medium, the true leaves grow out of the young buds. In addition, the excised segments of leaf stalk of Epipremnum aureum also can be cultured grown out young buds and true leaves on the same medium.