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Studies on Preparation of Bioactivity Parts of total Isoflavone Aglycones from Rhizoma Iridis Tectori

Yang Yongxun1,2,Dang Xiaoping1*,Chen Hulan1,Tan Yuzhu1(1.Pharmacy College,Chengdu University of Traditional Chinese Medicine,The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicines,Chengdu 611137,China; 2.School of Aplied and Chemical Engineering of Xichang College,Xichang 615000,China)  
Objective:To optimize the Preparation of bioactivity part of total isoflavone aglycones of Iris tectorum Maxim..Methods:The optimum absorption conditions of AB-8 macroporous absorption resin is studied by orthogonal design,and eluted with a gradient of EtOH in H2O to obtain glycosides and aglycones,glycosides were hydrolyzed by acid,the acid was eliminated by macroporous absorption resin.Results:The total isoflavone aglycones' purity have 59%~63%,yield 3.59%.Conclusion:The preparation is simple,convenient and reliable,and is suitable for utilization of industry.
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