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《亚太热带医药杂志(英文版)》 2013-01
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Preventive effects of sevoflurane treatment on lung inflammation in rats

Shu-Ye Song,Bing Zhou,Shuang-Mei Yang,Guo-Ze Liu,Jian-Min Tian,Xiu-Qin Yue~* Department of Anesthesiology,Xinxiang Medical College First Affiliated Hospital,Weihui 453100,Henan,China  
Objective:To observe the effects of sevoflurane treatment on lung inflammation in rats with lipopolysaccharide-induced acute lung injury(ALI).Methods:The rat model of ALI was established by intratracheal instillation of lipopolysaccharide(LPS).45 infantile SD rats[body weight(272±15) g]were randomly divided into 3 groups(n=15):control group,LPS group, sevoflurane group.NS(1 mL/kg) was instillated in rats'airways of control group;LPS(5 mg/ kg) was instillated in rats'airways of LPS group.Sevoflurane group rats received sevoflurane (2.4%) inhalation for a hour after LPS was instillated in rats'airways.Six hours after NS or LPS instillation,all rats were exsanguinated.Lung tissues were examined by HE staining.Expressions of TNF-αand ICAM1 mRNA were detected by semiquantitative RT-PCR techniques.The protein level of TNF-αand ICAM1 were assessed by western blot techniques.Results:In LPS group the permeability of lung tissues increased,organizational structure severely damaged and the alveolar wall turned thick,with interstitial edema and Europhiles infiltrated increasingly.The LPS group had higher mRNA expressions of TNF-αand ICAM1 than control group and sevoflurane group (P0.05),and LPS group had higher protein level of TNF-αand ICAMI than control group and sevoflurane group(P0.05).Conclusions:Sevoflurane treatment can attenuate lung inflammation in rats with lipopolysaccharide-induced acute lung injury.
【Fund】: Supported by Henan Province health science and technology creative talents innovation talents Foundation(Grant No.20114155)
【CateGory Index】: R614
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