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《亚太热带医药杂志(英文版)》 2013-12
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Effect of different anesthesia methods on erythrocyte immune function in mice

Wen-Long Zhang;Meng-Ying Liu;Zhen-Chen Zhang;Chen-Yang Duan;Department of Anesthesiology,No 175Hospital of PLA,Affiliated Southeast Hospital of Xiamen University;Department of Anesthesiology,Third Military Medical University,Student Cadets' Teams 2;Department of Anesthesiology,Southwest Hospital,Third Military Medical University;  
Objective:To explore effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice.Methods:The mice were anesthetized by isoflurane and ether inhalation,and also under intraperitoneal anesthesia with sodium pentobarbital and chloral hydrate,Blood was collected from the ventro-cardinal vein.Automatic blood cell analyzer was used for routine blood examination,and the canthine oxidase method was used to measure the superoxide dismulase(SOD) activity.Lipid peroxidation product malondialdehyde(MDA) was measured with TBA and glutathione peroxidase(GSH-Px) was measured with DTNB,and then the effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice was observed.Results:Hct level of chloral hydrate intraperitoneal injection group was significantly higher than the other three groups(P0.05).And the MDA levels in the pentobarbital sodium group were significantly higher than the other three groups(P0.05).SOD and GSH-Px of the chloral hydrate and sodium pentobarbital intraperitoneal injection group were significantly lower than the other two groups:RBC-C 3bRR and RBC-ICR of the chloral hydrate and sodium pentobarbital intraperitoneal injection group were significantly lower than the other two groups.Conclusions:Different drugs can induce changes in immune Function of mice at different levels.Isoflurane and ether have less damage to animal body,while chloral hydrate and sodium pentobarbital intraperitoneal injection have a certain inhibitory effect on the animal body respiratory system and can cause greater damage to the body.Therefore,the reasonable selection and control of anesthetics are very important in order to avoid the experimental errors caused by anesthesia.
【Fund】: supported by National Natural Science Foundation(No.81171035)
【CateGory Index】: R614
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Feng,TONG Yeling,DAI Guanhai,REN Zeming,ZHU Jianping,WANG Bobo(Zhejiang Institute of Traditional Chinese Medicine,Hangzhou 310007,Zhejiang,China);Effects of Propolis and Ginseng Soft Capsule on the Immunological Function of Mice[J];Chinese Archives of Traditional Chinese Medicine;2013-02
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 HU Zhi-ping;HUANG Cheng;Gannan Medical University;;The effect of electroacupuncture with different frequencies and number of stimuli on immune function in mice[J];Lishizhen Medicine and Materia Medica Research;2013-10
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