Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Conservative Dentistry》 2010-02
Add to Favorite Get Latest Update

Growth and biofilm forming capability of a wild-type Streptococcus mutans strain and a fluorescence protein reporter gene-labeled Streptococcus mutans strain

WANG Wei,HU Nan,SU Ling-yun,NI Long-xing(School of Stomatology,The Fourth Military Medical University,Xi'an 710032,China)  
AIM:To compare the growth and biofilm forming capability of a wild-type Streptococcus mutans strain(UA140) and a fluorescence protein reporter gene-labeled Streptococcus mutans strain(UA140-mrfp).METHODS:The growth of UA140 and UA140-mrfp in BHI broth were measured at different time points at OD600.Biofilm amount and structure were observed with scanning electron microscope(SEM).Data were analyzed by "repeated measurement design ANOVA" with SPSS 10.0.RESULTS:There was no significant growth difference between the 2 strains although the OD 600 value of UA140-mrfp was slightly lower than that of the UA140(P0.05).SEM demonstrated that there was no significant difference on the amount of biofilm between the two groups(P0.05).Furthermore,the structure of the biofilms was similar.CONCLUSION:UA140-mrfp could be used as a substitute for UA140 in the research of biofilm formationce in vitro model systems.
【Fund】: “十一五”国家科技支撑计划课题(2007BAI18B01);; 全军医药卫生科研基金课题(06MA236);; 军队十一五攻关课题(08G104)
【CateGory Index】: R378
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved