Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF CONSERVATIVE DENTISTRY》 1998-02
Add to Favorite Get Latest Update

The primary culture and the identification of human fetal osteoblasts

Li Dehua, Liu Baolin, Wu Junzheng et al School of Stomatology, The Fourth Military Medical University, Xi'an 710032  
Aim:Based on the advantages and disadvantages of enzyme-disgestion technique commonly used for the isolation of osteoblasts from bone tissue, we investigated the feasibility of the explant technique and its practicable value. Methods: The bone explants were harvested from the calvaria of 6-month fetuses after legal abortion and cultured in DMEM containing 50μg/ml of L- ascordic acid. The identification of derived cells was done by histochemical staining of alkaline phosphatase( ALPase) , immunohistochemical staining of osteocalcin, von- Kossa staining of calcified nodules and the observation of cell morphology. Results: After 5 days of cell culture,some triangle-shaped or short spindle-shaped cells migrated from the explants,and at the same time,the bone explants tended to break into small pieces.After 7 days, triangle- shaped or square- shaped cells appeared under the breaking explants.And finally, the cells reached confluency after 12 days.The derived cells were identified to be osteoblastic cells by the criteria of ALPase,osteocalcin and calcifing nodules formation. Conclusions: As to the primary isolation of osteoblasts from bone,the explant technique is a reliable, efficient and effective method.
【CateGory Index】: Q343.6,
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 9 Hits
1 Chen Jun, Yin Meizhen, Li Shipu Primary Hospital, Wuhan University of Technology, Wuhan 430070, China;Primary Culture and Identification of the Rat’s Osteoblasts in Vitro[J];Journal of Hubei Medical University;2005-05
2 Wang Ping,Wang Xiaojin,Fan Jihong,Yang Jucai.School of Stomatology,The Fourth Military Medical University,Xi'an(710032);The Biological Characteristics of Cultured Human Osteoblastilike Cells From Embryo[J];Stomatology;2001-04
3 Yin Meizhen, Li Shipu, Yuan Lin. Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China.;Culture in Vitro by Multiple Enzyme Digestion and Identification of Rat Osteoblasts[J];Orthopaedil Biomechanics Maternials and Clinical Study;2005-05
4 Li Dehua,Liu Baolin. Department of Oral and Maxillofacial Surgery,Stomatological College,Fourth Military Medical University, Xi'an 710032;A three-dimensional experimental model of endosseous implant material-human fetal osteoblasts culture[J];JOURNAL OF PRACTICAL STOMATOLOGY;2000-02
5 LIU Li,CHANG Hong,HUANG Guo-wei,WANG Yuan,BAI Shao-pei (College of Health of Tianjin Medical University,Tianjin300211,China);Establishment of experimental model of cultured rat's osteoblast in vitro[J];Journal of Tianjin Medical University;2004-01
6 LI Dehua,LIU Baolin,WU Junzheng,et al. Fourth Military Medical University,Qindu Stomatological College,Xi'an 710032;Effects of modified sandblasted surface on the bone adaptation pattern of dental implant[J];Journal of Modern Stomatology;2002-05
7 JIN Li-ming,GUO Wen-ting, Fan Sheng-di.College of Biological Science, Dalian Nationalities University, Dalian 116600, China;Some Questions of Bone Defect Healing Using Tissue Engineering[J];Medicine & Philosophy(Clinical Decision Making Forum Edition);2006-06
8 LIU Yue-qiang~1,LIU Kai-tai~2,YANG Xiao-yan~1,ZHONG Jin-jie,XU Yong-hua~3,ZHANG Dong-hui~3,ZHOU Ting-ting~2(1.Department of Labour and Environment Hygiene,College of Public Health,Xinjiang Medical University,Urumqi 830000;2.National Center for Disease Control and Prevention,Beijing 102200;3.Department of Animal Experiment,Urumqi General Hospital of Lanzhou Command,PLA,Urumqi 830000,China);Osteoblasts of Newborn Mouse Cultured in Vitro[J];Acta Laboratorium Animalis Scientia Sinica;2007-03
9 LI De hua *,LIU Bao lin, SONG Ying Liang, WU Jun zheng. *Department of Implantation, College of Stomatology, The Fourth Military Medical University, Xi′an 710032,China;Improvement of osseointegration of titanium dental implants by amodified sandblasted surface[J];Chinese Journal of Stomatology;2003-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Guihua ;Wang Qimin;Chen Long (Exercise Biochemistry Teaching and Research Section,BUPE, Beijing 100084);Comparative Analysis of Contents of Alkaline Phosphatase and Bone Mineral in Blood Neutrophile Granulocytes of Athletes[J];JOURNAL OF BEIJING UNIVERSITY OF PHYSICAL EDVCATION;1997-04
2 GOU Zhong-ping,DENG Dao-cang Chengdu University of Traditional Chinese Medicine (Chengdu, 610075, China);Effect On Bu-xu Hua-yu Prescription to Bone Marrow Depression due to Hemorrheological Indices of Carcinomatosis Mice after Chemotherapy[J];Journal of Chengdu Unversity of Tarditional Chinese Medicine;2003-04
3 Wang Xiangyu;Chen Changcai;Li Tielin;Zhu Cheng(Department of Neurosurgery,Zhujiang Hospital, First Military Medical University,Guangzhou, 510282)(Department of Neurosurgery,Changzheng Hospital,Second Military Medical University, Shanghai, 200003);Immunohistochemical study of endothelin in the cerebral artery and hypothelamus after experimental subarachnoid hemorrhage in rats[J];JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY;1997-02
4 ZHANG Hong 1 , WANG Ya-dong 2 , LAI Zhuo-sheng 21 Surgical Laboratory, 2 Institute of Digestive Disease of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Application of nitrocellulose membrane in cell culture and pathological techniques[J];Journal of First Military Medical University;2003-01
5 LI Ying-jun,ZANG Li, ZHANG Nai-sheng,GUO Shun-yuan (Faculty of Animal Medicine, Quartermaster University of PLA,Changchun,Jilin,130062,China);The Optimizing of Separated Method about PMN from Dairy Cattle Peripheral Blood[J];Progress In Veterinary Medicine;2005-07
6 XU Jian\|wen, WANG Wei, KANG Zhong\|han, et al (Department of Anatomy, Fujian Medical University, Fuzhou\ 350004, China)\;;Cultivation of Mouse Brain Microvascular Endothelial Cells in Vitro[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;2000-03
7 WANG Xiangju,YAN Hong,ZHANG Da ,an,et al./Institute of Forensic Sciences, Ministry of Public Security,Beijing 100038;A rapid method of detecting G1m(3) factor by using the immunochromatography assay[J];CHINESE JOURNAL OF FORENSIC MEDICINE;2000-02
8 ZHANG Jie1, XUE Qing-jie2, TANG Guang-feng1, PENG-Lei1 (1.Criminal Police department of Taian City Public Security Bureau, Taian 271000,China; 2.Jining medicinal college,Jining 272400,China);DNA Extraction from Old Bones[J];Journal of Forensic Medicine;2006-01
9 Pu Demin, Yin Ling, Hu Fe et al (Department of Obstetrics and Gynecology ,Tongji Hospital, Tongji Medical College, Huazhong polytechnic University Wuhan 430030 China) \;;DNA CONTENT, PCNA AND P53 EXPRESSION IN VULVA LICHEN SCLEROSUS AND THEIR RELATION WITH CELL PROLIFERATION[J];Chinese Journal of Histochemistry and Cytochemistry;2001-02
10 Zeng Xianjie,Kuang Xiaocong,Wen Guanmei, et al (Department of Head and Neck Surgery,the Affiliated Tumor Hospital of Guangxi Medical University,Nanning, 530021 China);EFFECT OF Ce(NO_3)_3 ON THE GROWTH OF HUMAN LARYNGEAL CANCER CELL LINE Hep-2[J];Journal of Guangxi Medical University;2001-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Zhi ying 1, WANG Xing 1△ , XIAO Jun jun 2, DONG Xiao min 2, MENG Shu cong 2, ZHAO Man 2 (1. Peking University School of Stomatology, Beijing 100081,China; 2. Department of Cell Biology & Genetics, Peking University);Culture of osteoblasts by serial explant culture and comparison of their characteristics[J];Journal of Peking University(Health Sciences);2004-01
2 YANG Liye 1 , LIU Xiangming 1 , SUN Bing 1 , HUI Guozhen 1 , MIAO Hongsheng 1 , LI Chaoyue 1 , FEI Jian 2 , GUO Lihe 21 Department of Neurosurgery , First Hospital of Suzhou University, Suzhou 215007, China; 2 Institute of Biochemistry and Cytobiology, Shanghai Life Science Academy, Chinese Academy of Science, Shanghai 200031, China;Expression of neuronal phenotypes by adipose tissue-derived stromal cells[J];Chinese Journal of Neuromedicine;2002-01
3 ZHANG Guanglei, ZHONG Taoxing, ZHANG Guozhen, MA Jie, LIU Peng, WANG Congzeng, JI Yuanof Education,Beijing University of Technology, Beijing, 100022, China);A study of surface modification of titanium alloy by chemicaltreatment following laser fusing[J];Material Science and Technology;2003-01
4 Su Baohui Ran Xu Ran Junguo Gou Li (Department of Inorganic Material, Sichuan University,Chengdu 610065,China);Study on Biological Properties of HA/β-TCP Biphase Calcium Phosphate Ceramic Materials[J];Rare Metal Materials and Engineering;2007-S2
5 YAN Ai min,LIU Li,CHEN Bing Li,et al. (Department of Orthopadies,Central Hospital of Handan City,Handan 056001,China);Primary culture and identification of the rabbit embryo osteoblasts[J];Journal of Traumatic Surgery;2002-S1
6 YANAi min ,LIULi,CHENBing li,etal. (DepartmentofOrthopadies,CentralHospitalofHandan ,HebeiProvince ,Handan 0 5 6 0 0 1,China);Experimental study of transplantation of rabbite mbryo osteoblasts for repair of bone defect of allogeneic rabbit[J];Journal of Traumatic Surgery;2003-02
7 LUAN Bo *, MEI Ju, ZHANG Bao Ren, HUANG Sheng Dong, LI Zhi Gang(Department of Cardiothoracic Surgery,Changhai Hospital,Second Military Medical University,PLA Institute of Cardiothoracic Surgery,Shanghai 200433,China);In vitro differentiation of bone mesenchymal stem cells into cardiomyogenic cells in cardiomyocytes microenvironment[J];Academic Journal of Second Military Medical University;2003-03
8 ZHENG Qi Jun, CAI Zhen Jie, YU Shi Qiang, YANG Guang, WANG Ya Yun, HU Jun Center of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;Experimental study on differentiation of mesenchymal stem cells in bone marrow to cardiomyocytes in vitro[J];Journal of The Fourth Military Medical University;2002-20
9 GAO Feng, CAI Zhen Jie, YU Shi Qiang, HU Jun, HU Qiao Xia, CHENG Liang Centre of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;In vitro induction of rat meseachymal stem cells into cardiomyocytes[J];Journal of The Fourth Military Medical University;2002-20
10 ZHANG Zheng~1, LIU Ying-Feng~1, CHENG He-Xiang~2, WANG Hai-Chang~2, GUO Wen-Yi~2, JIA Guo-Liang~2, ZHOU Lian~3 ~1Department of Cardiology, Zhujiang Hospital, South Medical (University), Guangzhou 510282, China, ~2Department of Cardiovasology, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China, ~3Northwest Institute for Non-ferrous Metal Research, Xi'an 710016, China;Effects of constant magnetic field on secretion and expression of ICAM-1 and VCAM-1 in endothelial cells and their adhesion rates with monocytes[J];Journal of The Fourth Military Medical University;2005-13
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Guilong HOU Yudong SHI Lingbo Department of Stomotology,Binzhou Medical University,Binzhou 256603;Effects of smokeless tobacco extract on the rat's osteoblasts in vitro[J];Journal of Binzhou Medical University;2009-02
2 ZHANG Ya-fei,WANG Hui,DU Rong-gui,LIN Chang-jian(State Key Laboratory for Physical Chemistry of Solid Surfaces,Department of Chemistry,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China);Effect of the etching treatments on titanium surface morphology[J];Journal of Functional Materials;2007-06
3 Wang Shengguo1,Zhou Li2,Wang Yanmin2,Chen Yangxi2,Ren Xiaohua3(1Department of Stomatology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010;2Department of Orthodontics,West China College of Stomatology,Sichuan University,Chengdu,Sichuan Province,610041;3Department of Stomatology,People's Hospital of Sichuan Province,Chengdu,Sichuan Province,610072,China);Effect of static magnetic field on proliferation and surface ultra-structure of osteoblasts in vitro[J];Journal of Third Military Medical University;2011-12
4 DENG Tian-yan1,WANG Shao-an,XIE Zuo-li,et al.1Jiangmen Hospital of Stomatology,Jiangmen 529000,China;Influence of Surface Microstructure of Titanium on Viability and Adhesion of Human Monocytes[J];Journal of Dental Prevention and Treatment;2011-06
5 Yin Meizhen(School of Medicine,Huangshi Institute of Technology,Huangshi Hubei 435003);An Improved Method of Rabbit Osteogenesis Cell Culture in Vitro[J];Journal of Huangshi Institute of Technology;2007-04
6 DONG Fei, DING Zhong-juan, NIU Tao. (Dept. of Prosthodontics, College of Stomatology, Kunming University of Medical Sciences, Kunming 650031, China);Effects of TiO_2 blasted and acid-etched titanium surfaces on oxide-film and osteoblast[J];West China Journal of Stomatology;2008-01
7 E Ling-ling,LIU Hong-chen,WANG Dong-sheng.(Stomatologic In-stitute,General Hospital of Chinese PLA,Beijing 100853,China);Primary culture and identification of neonatal rat's mandibular osteoblasts with modified repeating enzymatic digestion-adherent explants method[J];West China Journal of Stomatology;2009-02
8 CHEN Chang-qing,YIN Xiao-feng,LIAN Ke-jian,HUANG Li-xian,ZHANG Shan-tao,LI Qiang.Orthopaedics and Traumatology Center of PLA,175th Hosoital of PLA,Zhangzhou 363000,China;Osteoblasts and xenogenic deproteinized bone composite as bone substitute for lumbar posterolateral fusion[J];Journal of Spinal Surgery;2011-01
9 ZHAO Bing-jing,ZHAO Yue,QU Shuo,LV Ming-xia,WANG Zhi-ying.(The Second Affiliated Hospital of Liaoning Medical University,JinZhou,Liaoning 121000 China);Experimental study on the growth of osteoblasts with different titanium surface treatment[J];Chinese Journal of Prosthodontics;2009-05
10 TAN Xiao-bing 1), WU En-ge 1), ZHAO De-ping 2), JIA Wei 2) (1)Dept. of Stomatology, Kunming Medical College, Kunming 650031; 2) Clinical Medical Experiment Center, The 1st Affiliated Hospital of Kunming Medical College, Kunming 650032,China);Experimental Study of the Effect of Novel Bioceramics on Osteoblasts in Vitro[J];Academic Journal of Kunming Medical College;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved