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《Chinese Journal of Pharmaceutical Analysis》 2002-02
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Discussion about NaNO_2 - Al (NO_3)_3 - NaOH Colorimetry for Determination of Total Flavonoids

GUO Ya - jian, FAN Li, WANG Xiao - qiang and ZHANG Lan - zhen (Phytochemistry Department, Institute of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100029)  
Objective: To probe into the speciality of NaNO2 - Al ( NO3) 3 - NaOH colorimetry for determination of total flavonoids. Method: Make a series of flavonoids and non - flavonoids react with NaNO2, Al (NO3)3, NaOH solution, then mensurate their absorption curves at the range of 400-700 nm. Result: At the wavelength 500 nm, some flavonoids showed no λmax or very weak absorption, and to the opposite, some non -flavonoids have maximized absorption or have upper absorption. Conclusion: It is not exclusive to determine total flavonoids by u-sing NaNO2 - Al ( NO3) 3 - NaOH colorimetry with rutin as reference substance.
【Key Words】: colorimetry determination of total flavonoids
【CateGory Index】: R284
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