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《Chinese Journal of Pharmaceutical Analysis》 2002-05
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Determination of Biflavones from Selaginella by HPLC

LIU Hai - qing , LIN Rui - chao, FENG Fang and DANG He - qun ( Qinghai Institute for Drug Control, Xining 810000; National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050; China Pharmaceutical University, Nanjing 210009)  
bjective: To establish a HPLC method for the determination of biflavones from herbs of Selaginella Spring. Methods: The column was Elite - ODS (5μm, 150 mm ×4. 6 mm) ; the mobile phase consisted of methanol and phosphate buffer, the gradient condition was 0→6 min, methanol 47. 5%→70. 0% ; the column temperature was 40℃ ; the flow rate was 1. 5 mL · min-1 and the detection was done at 330 nm. Results: The average recoveries of amentoflavone, hinokiflavone and ginkgetin were 97. 05% , 98. 45% and 96. 88% ( RSD = 2. 1% , 2. 8% , 2. 3% , n =3) respectively. Amentoflavone was a common component and its contents from 12 species of Selaginella Spring were markedly different. It also showed from HPLC the difference in distributions and contents of hinokiflavone and ginkgetin from Selaginella Spring. Conclusion: This method was simple, sensitive and rapid and was very suitable for the determination of biflavones.
【CateGory Index】: R284
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【References】
Chinese Journal Full-text Database 10 Hits
1 SHI Zhixian, LIU Mei, YANG Yueqin and H U Fengzu(Northwest Plateau Institute of Biology, the Chinese Academy of Scienc es,Xining,810001);Advances in high performance liquid chromatography and related techniques of 200 1~2002 in China[J];Analytical Laboratory;2003-05
2 CHAI Yi-feng, WU Yu-tian and LI Xiang(Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Guohe Road 325, Shanghai 200433), Fenxi Shiyanshi,2004,23(6):71~92;Pharmaceutical Analysis I[J];Analytical Laboratory;2004-06
3 JIN Nan1,CHEN Ke-li2,CHEN Gang2,YANG Zhan-qiu3(1.Department of Pharmacy,the Hubei Institution Hospital,Wuhan 430071,China;2.Hubei Key Lab of Traditional Chinese Medicine Resource and Activity Study,Hubei College of Traditional Chinese Medicine,Wuhan 430064,China;3.Virus Research Institute,Medical College of Wuhan University,Wuhan 430071,China);Inhibitory Effect of Extracts from Selaginella Genus on Herpes Simplex Virus-Ⅰ Replication in Vitro[J];Herald of Medicine;2009-04
4 WU Cai-xia,ZHI Miao-li,HE Guang-dong,LEI Hao-zhi,KANG Wen-yi(Institute of Natural Drugs, Henan University, Kaifeng 475004,China)WU Cai-xia,KANG Wen-yi(Henan Center for Disease Control and Prevention, Zhengzhou 450003, China);Determination of Amentoflavone in Selaginella Tamariscina from Guizhou and Hunan by RP-HPLC[J];China Pharmacy;2008-15
5 Yu Jiandong, Hu Junying1, Cheng Xianglong and Ma Shuangcheng(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050; 1Guangzhou University of Chinese Medicine, Guangzhou, 510006);Study on the Quality Standard of Herba Selaginellae[J];Chinese Pharmaceutical Affairs;2008-08
6 LI Li1,2,CHEN Ke-li1,ZHU Tian-mi1,FAN Xiao-lei1 (1.Pharmaceutical Department,Hubei College of Traditional Chinese Medicine,Hubei Wuhan 430061,China;2.School of Chemical Engineering & Pharmacy,Wuhan Institute of Technology,Hubei Wuhan 430073,China);Inhibition of lipoxygenase by the extracts from the medicinal plants of Selaginella genus[J];Chinese Journal of Hospital Pharmacy;2006-12
7 FAN Xiao-lei1,WAN Ding-rong2,3,YE Cong-jin2,CHEN Ke-li2 (1.Wuhan Institute for Drug Control,Wuhan 430012,China;2.Hubei College of Traditional Chinese Medicine,Wuhan 430061,China;3.College of Life-Science,South-Central University for Nationalities,Wuhan 430074,China);Study on HPLC fingerprint characteristics of Selaginella plants[J];China Journal of Chinese Materia Medica;2007-20
8 ZHANG Jian-hua1,YANG Fan2,SHEN Jian2,LI Fu-shuang2,LIU Jian-feng2,TAN Gui-shan2,XU Kang-ping2(1.Center for Disease Control and Prevention of Dong'an County,Yongzhou Hunan 425900;2.School of Pharmaceutical Sciences,Central South University,Changsha 410013);Rapid detemination of amentoflavone in Selagingella by HPLC[J];Central South Pharmacy;2009-07
9 CUI Guohua SHI Ying GAO Bin YE Fang ZHOU Kexiang (Institute of Baiyunshan General Pharmaceutical Industry,Guangzhou 510515);TLC Qualitative Studies of 11 Species of Selaginella Medicinal Material[J];Traditional Chinese Drug Research & Clinical Pharmacology;2006-06
10 Lei Xiang1,Li Li1,Chen Keli1,Li Juan1,Yang Zhanqiu2(1 Hubei College of Traditional Chinese Medicine,Wuhan 430064;2 Virus Research Institute,Wuhan University,Wuhan 430071);Study on the inhibitory effect on Coxsackie virus B3 replication in vitro by the extracts from 7 medicinal plants of the Selaginella genus[J];Pharmacology and Clinics of Chinese Materia Medica;2007-05
China Proceedings of conference Full-text Database 1 Hits
1 Lei Xiang~1 Li Li~1 Chen Keli~1 Li Juan~1 Yang Zhanqiu~2 (~1 Hubei College of Traditional Chinese Medicine,Wuhan 430064;~2 Virus Research Institute,Wuhan University,Wuhan 430071);Study on the inhibitory effect on Coxsackie virus B3 replication in vitro by the extracts from 7 medicinal plants of the Selaginella genus[A];[C];2007
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Xiao-qing,SU Yu-cai,CHEN Zheng-bin(Department of Biology Science and Technology,Zhangzhou Normal College,Zhangzhou,Fujian 363000,China);Extraction and purification of antibacterial polysaccharide from Selaginella tamariscina[J];Subtropical Agriculture Research;2009-02
2 ZHANG Xiu-li,WANG Xin-ying,SHI Lan-xiang(College of Materials Science and Engineering,China Geology University,Beijing 100083,China);Determination of 7-amido acetoxy eicosanoic acid by ultraviolet spectrophotometric method[J];Chemical Engineer;2006-07
3 LUO Liu-bao,XIE Zhi-peng(1.Jiangxi College of Applied Technology,Jiangxi Ganzhou 341000,China;2.Chemistry School of Jiangxi University of Science and Technology,Jiangxi Ganzhou 341000,China);Study on Xanthine Oxidase Inhibitory Activity in Chinese Traditional Medicines by Spectrophotometry[J];Chemical World;2009-05
4 LUO Cheng,ZHOU Da,LU Xiao-xiang*(Biotechnology and Food Science College,Tianjin University of Commerce,Tianjin Key Laboratory Food of Biotechnology,Tianjin 300134,China);Research progress in antioxidant mechanism of natural products[J];Science and Technology of Food Industry;2009-04
5 LIU Ning 1, SHENG Rong 2, TAN Jing2 (1.College of Bioengineering, Southwest Jiaotong University, Chengdu 610031, China; 2.The Hospital Affiliated to Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China);Content determination of puerarin in Sichuan Radix thomsonii puerariae by HPLC[J];China Medical Herald;2009-18
6 YIN Dan,CHEN Ke-li (Hubei College of TCM Key Laboratory of TCM Resource and Foumulation of the Ministry of Education,Hubei Wuhan 430065,China);Study on the inhibitory action on CVB3 replication in vitro by the extracts from Selaginella moelledorfii Hieron[J];Chinese Journal of Hospital Pharmacy;2009-04
7 YIN Dan,CHEN Ke-li (Hubei College of TCM Ministry of Education Key Laboratory of Resource and Compound Recipe of Chinese Medicines,Hubei Wuhan 430065,China);Study on the inhibitory action on HSV-I replication in vitro by the extracts from Selaginella moelledorfii[J];Chinese Journal of Hospital Pharmacy;2009-05
8 ZHANG Jian-hua1,YANG Fan2,SHEN Jian2,LI Fu-shuang2,LIU Jian-feng2,TAN Gui-shan2,XU Kang-ping2(1.Center for Disease Control and Prevention of Dong'an County,Yongzhou Hunan 425900;2.School of Pharmaceutical Sciences,Central South University,Changsha 410013);Rapid detemination of amentoflavone in Selagingella by HPLC[J];Central South Pharmacy;2009-07
9 Zhao Shanli Chai Manchao HenanTumorHospital(450007);Study of Chemical Constituents of Selaginell a Labordei[J];Chinese Medicine Modern Distance Education of China;2009-08
10 SUN Ying-zheng1,2,CHE Ke-li2,YIN Dan2,XU Jia-cheng2(1.Biotechnology Department,Zilang Professional College of Technolgy,Jiangsu Nantong,226002,China;2.Hubei College of TCM,Key laboratory of Traditional Chinese Medicine Resource and Compound Ministry of Education,Hubei Wuhan,430065,China);Inhibiting action of bisflavones from Selaginella labordei on Bel-7402 cells[J];Chinese Journal of Hospital Pharmacy;2009-14
China Proceedings of conference Full-text Database 1 Hits
1 LI Li~(1,2),CHEN Ke-li~1,XU Jia-cheng~1,LEI Xiang~1 (1.Key Laboratory for Traditional Chinese Medicine Resource and Compound Prescription of Ministry of Education,Hubei College of Traditional Chinese Medicine,Wuhan 430065,China; 2.Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan Institute of Technology, Wuhan 430073,China);Inhibition of Xantine Oxidase by the biflavonoids from the medicinal plant of Selaginella labordei[A];[C];2008
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