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《Chinese Journal of Pharmaceutical Analysis》 2008-01
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HPLC simultaneous determination of the four nitroimidazoles formulation

WU Mei-xiang~1 LI Huan-de~(2*) (1.Chenzhou Institute for Drug Control,Chenzhou 423000,China;2.The Second Xiangya Hospital of Central South University,Changsha 410011,China)  
Objective:To establish a checked-technology of HPLC for four nitroimidazoles formulation in the same position.Methods:The HPLC analysis was carried on a Thermo C_8(250 mm×4.6 mm,5μm).The mobile phase was methanol(A)-50 mmol·L~(-1) potassium dihydrogen phosphate(B)with gradient elution[0-30 min,A-B(5 :95)→A-B(20:80)]at a flow rate of 1.0 mL·min~(-1),inject 20μL sample into for analysis.The detection wavelength was 310 nm and the column temperature was 40℃.Results:The established technology can be used for the analysis of the four nitroimidazoles formulation(metronidazole,tinidazole,ornidazole,secnidazole).The com- pounds were identified according to the chromatographic behavior and mass spectral data by comparison with those of reference substances.We have found that the contents of four nitroimidazoles formulation were 96.0%,82.1%, 38.0%,100.1%,respectively.Conclusion:The technology is high specificity,accurate,simple,and fast.
【CateGory Index】: R927
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