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《Chinese Journal of Pharmaceutical Analysis》 2008-07
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A model to screen 5α-reductase inhibitors in vitro by RP-HPLC

YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China)  
Objective:To develop an RP-HPLC model in vitro for screening 5α-reductase inhibitors.Methods: The 5α-reductase was abstracted from the liver of rat.The activity of reductase was determined by the change in testosterone concentration using RP-HPLC.The chromatographic conditions were:column,Diamonsil~(TM)C_(18)(250 mm×4.6 mm,5 μm);mobile phase,methanol-water-acetic acid(70:29.5:0.5).flow rate,1.0 mL·min~(-1); column temperature,30 ℃;detection wavelength,230 nm.Results:Testosterone was effectively separated from other component under the chromatographic conditions.Epristeride,one effective 5α-reductase inhibitor,inhibited the activity of 5α-reductase in a dose-dependent manner.Conclusion:The method is simple,accurate and suitable for screening of 5α-reductase inhibitor in vitro.
【Key Words】: HPLC 5α-reductase inhibitor testosterone
【CateGory Index】: R96
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