Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Pharmaceutical Analysis》 2008-07
Add to Favorite Get Latest Update

A model to screen 5α-reductase inhibitors in vitro by RP-HPLC

YAN Dong-mei TU Ling-lan LI Wen-jun (Zhejiang Academy of Medical Sciences,Hangzhou 310013,China)  
Objective:To develop an RP-HPLC model in vitro for screening 5α-reductase inhibitors.Methods: The 5α-reductase was abstracted from the liver of rat.The activity of reductase was determined by the change in testosterone concentration using RP-HPLC.The chromatographic conditions were:column,Diamonsil~(TM)C_(18)(250 mm×4.6 mm,5 μm);mobile phase,methanol-water-acetic acid(70:29.5:0.5).flow rate,1.0 mL·min~(-1); column temperature,30 ℃;detection wavelength,230 nm.Results:Testosterone was effectively separated from other component under the chromatographic conditions.Epristeride,one effective 5α-reductase inhibitor,inhibited the activity of 5α-reductase in a dose-dependent manner.Conclusion:The method is simple,accurate and suitable for screening of 5α-reductase inhibitor in vitro.
【Key Words】: HPLC 5α-reductase inhibitor testosterone
【CateGory Index】: R96
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 SUN Zu-yue,WU Jian-hui, TU Zeng-hong, ZHU Yan, ZHONG En-hong (National Laboratory of Contraceptive and Devices Research, Shanghai Institute of Planned Parenthood Research; National Evaluation Centre for the Toxicology of Fertility Regulating Drugs, Shanghai 200032, China. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica , Chinese Academy of Science, Shanghai, Shanghai 200031, China);A Convenient and Rapid Model in vitro to Screen Steroid 5 alpha-reductase Inhibitors[J];Shanghai Laboratory Animal Science;2002-04
2 Kuang Gang,Zou Wenjun,Gao Xiaoping1(Chengdu University of TCM, Chendu 610075;1 New Drug Screening Center of Chengdu Diao Group, Chendu 610041);Establishment and application of a non-isotope model in vitro for screening 5α-reductase inhibitor[J];Pharmacology and Clinics of Chinese Materia Medica;2005-04
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Jian, GAO Xiao ping (Institute of Materia Medica, Diao Pharmaceutical Group, Chengdu 610041,China);Expression and Activity Assay of Human Steroid 5 Alpha-Reductase Type Ⅱ in CHO Cells[J];Pharmaceutical Biotechnology;2004-05
2 LIU Ben~1,CHANG Yi-ling~2,CHEN Ke-li~3(1.Ningbo School of Technology,Zhejiang University,Ningbo 315100,China;2.Zhejiang Pharmaceutical College,Ningbo 315100,Chian;3.Hubei College of Traditional Chinese Medicine,Wuhan 430061,China);Progress in the Study of 5α-Reductases Used as A Target in Natural Medicine Screening[J];Pharmaceutical Biotechnology;2006-06
3 LI Xiao-Lin, LI Xiao-Mei, LU Yan-Juan, et al . Experimental Center for Biochemistry and Molecular Biology, Basic Medical College of Jilin University, Changchun 130021, Jilin, China;The content changes of SOD and MDA in important organic tissues of adult diabetic rat[J];Chinese Journal of Gerontology;2005-04
4 Kuang Gang,Zou Wenjun,Gao Xiaoping1(Chengdu University of TCM, Chendu 610075;1 New Drug Screening Center of Chengdu Diao Group, Chendu 610041);Establishment and application of a non-isotope model in vitro for screening 5α-reductase inhibitor[J];Pharmacology and Clinics of Chinese Materia Medica;2005-04
5 DING Wei, ZOU Da-jin(Changhai Hospital affiliated to The Second Military Medical University,department of endocrinology, Shanghai 200433, China);The Progress of the Research on the Key Role of 5a-reductase in Androgen-induced Acne[J];The Chinese Journal of Dermatovenereology;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 JIA Yue, WANG Xiao dong, CUI Yu gui, DI Fu song;Roles of 5 α-reductase in male reproduction[J];Journal of Reproductive Medicine;2003-02
2 WU Jian-hui, ZHU Yan, SUN Zu-yue (Nation Evaluation Centre for the Toxicology of Fertility Regulating Drug, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China);Epristeride: A New Drug for Benign Prostatic Hyperplasia[J];Progress In Pharmaceutical Sciences;2002-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved