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《Chinese Journal of Pharmaceutical Analysis》 2008-10
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ICP-MS determination of intracellular cisplatin in human lung ladenocarcinoma cell and its application of drug-resistance

WU Cui-fang~1 LIU Shi-kun~(1**)ZHOU Yu-lu~1 PEI Qi~1 LI Bang-rui~2(1.Department of Pharmacy,the Third Hospital of Xiangya,Central South University,Changsha 410013,China 2.Hunan Center for Disease Control and Prevention,Changsha 410005,China)  
Objective:Determination of intracellular cisplatin in human lung ladenocarcinoma cell and study the re- lationship of drug-resistanee and its content.Methods:The drug-resistance of A549/DDP to DDP was tested by MTT assay.The content of intracellular cisplatin in cell was tested by inductively coupled plasma mass spectrometry (ICP-MS).Results:The calibration curve was linear when the concent of the Pt were in the range of 0.5-50 μg ·L~(-1) (r=0.9997).The detection limit of Pt was 0.029 μg·L~(-1).The average rate of recovery of Pt was 98.2% -99.7%,with RSD 0.41%-4.7% (n=5).The accumulation of DDP in A549/DDP were remarkably lower than these in A549 cell.Conclusion:ICP-MS determination of the content of intracellular cisplatin in human lung lade- nocarcinoma cell is established for the first time.The method is accurate,sensitive and simple.It can be used for the determination of the content of intracellular cisplatin and the study of resistance of human lung ladenocarcinoma cell to cisplatin.
【Fund】: 湖南省教育厅科学研究项目(NO04C432)
【CateGory Index】: R734.2
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