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《Chinese Journal of Pharmaceutical Analysis》 2012-05
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Isolation and identification of isoquercitrin andHPLC fingerprint study of Herba Emiliae

SHI Xin1,WANG Li-sheng1,PANG Sai2,DUAN Man1,YIN Yong1(1.Guangxi University,Nanning 530004,China;2.Guangxi Traditional Chinese Medical University,Nanning 530001,China)  
Objective:To isolate the flavonoid compound isoquercitrin from Herba Emiliae and establish the HPLC fingerprint of Herba Emiliae.Methods: Isoquercitrin was isolate from Herba Emiliae by semi-preparative HPLC,and its chemical structures was elucidated by 13C-NMR,1H-NMR and MS.The fingerprint was analyzed by HPLC;chromatographic conditions: Suzhou universal C18(250 mm×4.6 mm,5 μm) chromatographic column was adopted,the mobile phase was acetonitrile-0.4% acetic acid with elution gradient at the flow rate of 1 mL·min-1,the detection wavelength was set at 273 nm.Result: The purity of isoquercitrin was above 98%.In accordance with the provisions of China pharmacopoeia,11 batches of Herba Emiliae fingerprint had good similarity.Sixteen common peaks in chromatograms were determined.Conclusion: Semi-preparative HPLC is a simple and efficient method to prepare isoquercitrin.Fingerprint analysis method,being with high accuracy and repeatability,can be used in the quality control of Herba Emiliae.
【CateGory Index】: R284
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