Comparison of the contents of organic acids and coumarin compounds in different parts of Notopterygium incisum
LIU Wei-gen1,3,WANG Liang-sheng2,ZHOU Guo-ying1,XU Wen-hua1, YANG Lu-cun1,ZHU Man-lan4(1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;2.Beijing Botanical Garden,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China; 3.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;4.Nanjing Agriculture University,Nanjing,210095)
Objective: To investigate the distribution characteristics of the main effective constituents in different parts(root and rhizome,stem,leaf,petiole,seed) of Notopterygium incisum,so as to provide scientific basics for quality evaluation,exploitation and sufficient application of this herb.Methods: The contents of chlorogenic acid,ferulic acid,nodakenin,psoralen,bergapten,imperatorin,isoimperatorin and notopterol in different parts of N.incisum were determined by high performance liquid chromatography(HPLC) coupled with photodiode array detector(DAD) using a Tosoh TSK gel ODS-80Ts QA C18 column(250 mm×4.6 mm,5 μm).The mobile phase consisting of methanol and 0.3% acetic acid in a gradient elution mode was carried out as follows: 0 min→15 min→17 min→45 min→55 min→70 min→75 min→80 min,methanol 25%→38%→38%→50%→75%→85%→25%→25%.The analysis was done at a flow rate of 0.6 mL·min-1,the injection volume was 10 μL and the column temperature was 30 ℃.Results: The contents of the main effective compounds in different parts of N.incisum varied greatly from each other.Notopterol and isoimperatorin were mainly concentrated in the root and rhizome,while chlorogenic acid was widely distributed in the whole plant.The contents of nodakenin,psoralen and bergapten were found to be very low in all parts of the plant and psoralen was mainly found in the leaf and petiole of N.incisum.Imperatorin was not detected in any parts of this plant.Conclusion: The total content of the main effective compounds in root and rhizome of N.incisum was much higher than that in other parts of this plant,which was in accordance with the traditional therapy that only the root and the rhizome of N.incisum were selected as the medicinal part of this plant.Besides,it should be noted that the aerial part also showed some medicinal value to be developed in view of its high content of chlorogenic acid and other unknown components.
【Fund】： 中国科学院“西部之光”人才培养计划项目(2007年);; 科技部科技人员服务企业行动项目(2009GJG20016)资助
【CateGory Index】： R284.1
【CateGory Index】： R284.1