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《Chinese Journal of Pharmaceutical Analysis》 2014-02
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Identification and simultaneous determination of caffeoylquinic acids in Huahong capsules

SHEN Shou-mao;SHEN Lian-gang;ZHANG Jing;LI Guang-zhi;ZHANG Yan-hua;LIU Chun-ming;SI Jian-yong1#;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;School of Pharmacy,Yancheng Teachers' University;Guangxi Huahong Pharmaceutical Co. Ltd.;  
Objective: To analyze the caffeoylquinic acids and to develop an HPLC method for simultaneous determination of neochlorogenic acid,chlorogenic acid and cryptochlorogenin acid in Huahong capsules. Methods: A UPLC / Q- TOF- MS / MS method was developed to rapidly identify the caffeoylquinic acids in Huahong capsules. And the UPLC separation was performed on a Thermo BEH C 18 column( 2. 1 mm × 100 mm,1. 7 μm) with 0. 1% formic acid acetonitrile- 0. 1% formic acid as the mobile phase in gradient elution at a flow rate of 0. 4 μL · min- 1. The column temperature was set at 40 °C. The MS spectrometry detection was performed with electrospray ionization in negative ion mode,and using the following operating parameters: scan range,100- 1500 m / z; capillary voltage,2. 5 kV; skimmer voltage,40 kV; ion source temperature,120 °C; drying gas temperature,450 °C and sample collision energy,15- 40 eV. Besides,HPLC was performed on a Phenomenex C18( 250 mm ×4. 6 mm,4 μm)column with 0. 1% formic acid acetonitrile- 0. 1% formic acid as the mobile phase in gradient elution( 0- 7 min,5% →8% A; 7- 17 min,8% →15% A; 17- 45 min,15% →30% A; 45- 55 min,30% →50% A; 55- 60 min,50% →100% A) at a flow rate of 1. 0 mL·min- 1. The detect wavelength was set at 326 nm,and the column temperature was set at 30 °C. Results: Based on the UPLC / Q- TOF- MS / MS analysis,four caffeoylquinic acids,neochlorogenic acid,chlorogenic acid and cryptochlorogenin acid and isochlorogenic acid A,were identified from Huahong capsules. In addition,by using HPLC method,the correlation between the concentration and the chromatographic peak area of neochlorogenic acid,chlorogenic acid and cryptochlorogenin acid was linear in ranges of 3. 54-176. 80 μg·mL- 1( r = 0. 9999),6. 98- 348. 80 μg·mL- 1( r= 0. 9999),4. 06- 202. 80 μg·mL- 1( r =0. 9999),respectively. The average recoveries of the three caffeoylquinic acids were in the range of 101. 5%-102. 2%,and the relative standard deviations( RSD) were in the range of 1. 9%- 2. 4%. Conclusion: The UPLC /Q- TOF- MS / MS and HPLC methods do not only pave a way for further understanding of the chemical constituents in Huahong capsules,but also provide scientific evidences for the quality control of Huahong capsules.
【Fund】: 国家“重大新药创制”科技专项(Nos.2011ZX09307-002-01 2012ZX09301-002-001)
【CateGory Index】: R284.1
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Chinese Journal Full-text Database 10 Hits
1 ZHANG Yu-ping and HUANG Ke-long(Centural South University,Changsha 410083);Study on HPLC method for determination of five kinds of organic acids in flos lonicerae[J];Chinese Journal of Analysis Laboratory;2007-07
2 Wei Feiyan Zhang Yanhua (Guangxi Huahong Pharmaceutical Co.Ltd.,Liuzhou,545007) Si Jianyong (Institute of Medicinal plant Development Beijing 100193) Jia Xiaoguang (Xinjiang Institute of Chinese Materia Medica and Ethnical Materia Medica,Wulumuqi 830002);A Quality Standard Study for Huahong Capsule[J];World Science and Technology-Modernization of Traditional Chinese Medicine and Materia Medica;2008-04
3 HU Zhong-qiu,HONG Xiao-di,YUE Tian-li (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China);Extraction of Chlorogenic Acid from Fruit of Chaenomeles sinensis Koehne and Evaluation of Its Antibacterial Activity[J];Food Science;2010-24
4 SHI Xin1,WANG Li-sheng1,PANG Sai2,DUAN Man1,YIN Yong1(1.Guangxi University,Nanning 530004,China;2.Guangxi Traditional Chinese Medical University,Nanning 530001,China);Isolation and identification of isoquercitrin andHPLC fingerprint study of Herba Emiliae[J];Chinese Journal of Pharmaceutical Analysis;2012-05
5 LIN Jun~1,HE Ping~1,QUAN Min-min~2,HE Wei-long~2(1.Department of Pharmacology,Guangxi Medical University,Nanning 530021,China;2.Guangxi Huahong Pharmaceutical Co.,Ltd.,Liuzhou,545007,China);Pharmacodynamics of Huahong Capsule[J];Chinese Traditional Patent Medicine;2006-03
6 ZHANG An ling 1, MA Qiong 2, GAO Jin ming 1, ZHANG Kang jian 1, WANG Lan 1 (1 Key Laboratory of Development and Application of Resources of Medicinal Plant in Shanxi Province, Northwest Science and Technology University of Agriculture and Fores;Studies on bioactivities of chlorogenic acid and its analogues[J];Chinese Traditional and Herbal Drugs;2001-02
7 ZHANG Yan,ZHANG Hong-bin,CHEN Guang-zhou,WANG Yan(Department of Life and Science,Qiongzhou College,Wuzhishan 572200,Hainan,China);Determination of Tannins and Chlorogenic Acid of Different Vegetative Organs in Emilia sonchifolia(Linn.) DC[J];Chinese Archives of Traditional Chinese Medicine;2010-01
8 GUI Yi1,XU Li-hua2,LIN Yong-qiang2(1.Beijing North Hospital,Beijing 100089,China;2.Shandong Institute for Food and Drug Control,Jinan 250101,China);HPLC method for simultaneous determination of three caffeoylquinic acids in Laiyang pear clear paste[J];Chinese Journal of Pharmaceutical Analysis;2012-09
9 WU Ting1,ZHANG Jun 1,ZHOU Ling 2,JU Wen-zheng1,TAN Heng-shan3(1.Department of Clinical Pharmacology,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210009,China; 2.Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China; 3.Department of Clinical Pharmacology,General Hospital of Armed Forces of Nanjing,Nanjing 210002,China);Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC[J];Chinese Journal of Experimental Traditional Medical Formulae;2012-22
10 SHEN Shou-mao;ZHANG Jing;LI Guang-zhi;LIU Chun-ming;LU Hui;SI Jian-yong;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College;School of Pharmary,Yancheng Teachers' University;Guangxi Huahong Pharmaceutical Co.Ltd.;;Chemical Constituents of the Aerial Parts of Emilia sonchifolia(Ⅱ)[J];Chinese Pharmaceutical Journal;2013-21
Chinese Journal Full-text Database 10 Hits
1 YU Xiao-liang,WANG Jian-hua(College of Agronomy,Shandong Agricultural University,Taian 271018,China);Determination of Effective Components in Chaenomeles Fruits[J];Shandong Agricultural Sciences;2012-02
2 JIN Guang-ming1,2,PENG Li-juan1,2,QI Yu1,2,DING Chang-hong3 1.College of Animal Science,Anhui Science and Technology University,Fengyang 233100;2.Anhui Key Laboratory of Poultry Disease Prevention and Surveillance,Fengyang 233100;3.Anhui Anfengtang Animal Medicine Co.,Ltd.,Hefei 230000;Effects of Chinese Herb Extract on Performance of Local Chicken[J];畜牧与饲料科学(英文版);2009-02
3 LAI Pu-hui et al(College of Chemistry,Shaaxi University of Technology,Hanzhong,Shaanxi 723001);GC-MS Analysis of the Composition of Petroleum Extraction from Stem of Panax Japonicus[J];Journal of Anhui Agricultural Sciences;2008-23
4 LI Hong-fang et al (State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany of Chinese Academy of Sciences,Kunming,Yunnan 650204);Research Progress on the Chemical Components and Pharmacological Action of Polygonum perfoliatum L.[J];Journal of Anhui Agricultural Sciences;2008-27
5 CAI Zheng-jun et al(Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province,Hubei Institute for Nationalities,Enshi,Hubei 445000);Study on in vitro Antibacterial Activity of Caulophyllum robustum Maxim.[J];Journal of Anhui Agricultural Sciences;2008-35
6 WANG Shi-kuan et al (Institute of Biological Engineering,Sichuan Ligong College,Zigong,Sichuan 643000);Research on the Extraction of Chlorogenic Acid from Sweet Potato Leaf and its Antibacterial Efficiency[J];Journal of Anhui Agricultural Sciences;2010-11
7 ZHAO Jing et al(College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000);Determination of Chlorogenic Acid in Eucommia Ulmoides of Different Genotypes[J];Journal of Anhui Agricultural Sciences;2011-04
8 LI Cheng-bin1,2,LI En1,ZHENG Yan1,2,SHI Mei1,WU Qiong1(1.College of Life Sciences,Anhui Normal University,Wuhu 241000,China;2.Institute of Traditional Chinese Medicine Resources,Anhui Normal University,Wuhu 241000,China);Analysis of Nutrient Components in Luffa Cylindrical Stem and Leaf[J];Journal of Anhui Normal University(Natural Science);2009-01
9 FAN Zhenyu,ZHANG Liqiong,LI Lianhua(Department of Chemistry and Life Science,Ankang University,Ankang725000,Shaanxi,China);Effect of Different Medium on Inducement and Differentiation of Nicandra Physaloides[J];Journal of Ankang University;2008-05
10 PENG Qiong,SUN Yan-juan,YANG Zhen-de,LI Ming,ZHOU Jian-mei(Forestry College of Guangxi University,Nanning,Guangxi 530004,China);The Antimicrobial Activity of Tadehagi triquetrum(L.) Ohashi Extract and Its Preservative Function on Cut Carnation[J];Northern Horticulture;2009-03
China Proceedings of conference Full-text Database 8 Hits
1 SHEN Wei-Zhi[1,2], LIAO Sen-Tai12*, ZOU Yu-Xiao1, LIU Ji-Ping2, CHEN Zhi-Yi1, YANG Chun-Ying1 (1. Sericulture and Farm Produce Processing Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou 510610;2. College of Animal Science, South China Agricultural University, Guangzhou 510642);Study on the Change Law of Polyphenol Components in Mulberry Leaves of Different Varieties[A];[C];2009
2 Liang Qiao Wenhan Lin Congyuan Gao Qifeng Shen (Beijing Medical University 100083) Guangzhong Tu Libin Ma (Beijing Institute of Microchemistry 100091);THE STUDY ON THE STRUCTURE OF NEW IRIDOID GLYCOSIDES BY NMR[A];[C];1994
3 HU Run-huai WAN Yan (Zhejiang Forestry University,Zhejiang Lin' an 311300) (Pharmacy college,Henan University of Traditional Chinese Medicine,Zhengzhou 450008);The Determination of the content of chlorogenic acid in Folium Lonicerae in different regions and periods[A];[C];2007
4 ;Prepare Fufanghongteng Granules and Study on Quality Control Method[A];[C];2009
5 ;Prepare Fufanghongteng Granules and Study on Quality Control Method[A];[C];2010
6 CHEN Qiang (Department of Traditional Chinese Medicine, Military Region Institution hospital, Chengdu 610011,China);Using Ultraviolet Spectrophotometry to Determine the Content of Arteannuin in Artemisia annua L.in different areas in Chongqing[A];[C];2008
7 YUAN Xiao-yan GAO Ming-zhe XIAO Hong-bin TAN Cheng-yu DU Yi-guang (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023,China);Study on Extraction Process of Chlorogenic Acid in Helianthus tuberosus Linn leaves[A];[C];2008
8 LI Zhen-yu ,ZHI Hai-juan,ZHANG Fu-sheng,QIN Xue-mei;Research on metabolomic profiling and the antitussive and expectorant effect of different parts(bud,leaf,root) from Tussilago farfara L.[A];[C];2012
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xiuxin1,WANG Ronghua2,YANG Xiuping1(1 College of Forestry,Northwest SciTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 College of Horticulture,Northwest SciTech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);An analysis of flavonoids in Emilia sonchifolia[J];Acta Botanica Boreali-occidentalia Sinica;2003-04
2 WU Qing-hua1, LU Hui-zhen1, HU Dong-nan1, HAN Shi-yu2, WEI Fei-peng2.Guangxi Botanical Garden of Medicinal Plant、Institute of Guangxi Medicinal Plant、The Guangxi Brach,Institute of Medicinal Plant,Chinese Academy of Medical Sciences (Nanning,530023);2.Guangxi Huahong Pharmaceutical Co., Ltd (Liuzhou 545007);Studies on artificial cultivation of Emilia sonchifolia[J];Guangxi Medical Journal;2006-06
3 Zhang Kangjian Wang Yaqin Ma Xihan Wang Lan Zhang Tan (Northwestern University of Forestry,Yangling 712100);AN ECOLOGICAL STUDY ON SECONDARY METABOLITES OF THE LEAVES OF EUCOMMIA ULMOIDES[J];SCIENTIA SILVAE SINICAE;1999-06
4 Zhang Kangjian\ Ma Xihan\ Ma Mei\ Wang Lan\ Zhang Tan (Northwestern College of Forestry\ Yangling 712100);A STUDY ON DYNAMIC ACCUMULATION OF METABOLITES DURING THE GROWTH OF EUCOMMIA ULMOIDES OLIV[J];SCIENTIA SILVAE SINICAE;1999-02
5 JIANG Hai-qiang1,WANG Jian-ping2,LIU Yu-hong2,GONG Li-li1,LIU Hong-yan1(1.Experiment Center,2.College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China);Separation and Identification of Chemical Compositions fromBidens bipinnata L.[J];Food and Drug;2008-09
6 Ding Zhengyi Department of Instrument Analyses, Capital Institute of Medicine;Determination of Rutin Content in Two Groups Coexistence of Rutin and Quercetin with Double Wavelengh Method[J];Journal of Capital University of Medical Sciences;1992-01
7 MENG Fan-hao,CHEN Qiu-chen,WANG Lin,LIU Zhen,SUN Ye-zhi(School of Pharmacy,China Medical University,Shenyang 110001,China);Determination of quercetin in Herba Emiliae by HPLC[J];Chinese Journal of Biochemical Pharmaceutics;2007-06
8 LI Jun-sheng,YAN Liu-juan,SU Hui-wu,LIN Zheng(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006,China);Study on Separations of Emilia sonchifolia Flavonoids and Their Antibacterial Activities[J];Food Science;2007-09
9 SHU-YUAN LI#, CUI-QING CHANG#,2, FU-YING MA*, AND CHANG-LONG YU# #Division of Nutrition and Biochemistry, Institute of Sports Medicine, Third Hospital, Peking University, Beijing 100191, China; *Department of Pediatrics, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China;Modulating Effects of Chlorogenic Acid on Lipids and Glucose Metabolism and Expression of Hepatic Peroxisome Proliferator-activated Receptor-α in Golden Hamsters Fed on High Fat Diet[J];生物医学与环境科学(英文版);2009-02
10 YUAN Bo, LI Lei, LI Fa-mei(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China);Determination of the contents of chlorogenic acid and caffeic acid in the compound Daqingye Injection by RP-HPLC[J];Journal of Shenyang Pharmaceutical University;2004-02
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