Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Pharmaceutical Analysis》 2014-05
Add to Favorite Get Latest Update

Simultaneous determination of cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ_A in ethanol extract of Salviae Miltiorrhizae Radix et Rhizoma by ultra-performance liquid chromatography

TANG Yao;LI Zuo-gang;WANG Xiu-wen;WANG Ju-feng;LI Bo;Department of Drug Metabolism and Pharmacokinetics,National Center for Safety Evaluation of Drugs,National Institutes for Food and Drug Control;School of Pharmacy,China Pharmaceutical University;  
Objective: To establish an ultra-performance liquid chromatography method for simultaneous determination of three representative tanshinones( cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA) in ethanol extract of Salviae Miltiorrhizae Radix et Rhizoma. Methods: The method was performed on a Waters Acquity UPLC BEH Shield RP18( 1. 7 μm,2. 1 mm × 100 mm) analytical column employed with a guard column of Phenomenex SecurityGuard C18Cartridges Polar-RP( 4 mm × 3. 0 mm) at the column temperature of 35 ℃. The gradient mobile phase consisted of acetonitrile( A)-5% acetonitrile aqueous solution containing 0. 1% formic acid( pH 2. 5)( B) with a flow rate of 0. 25 mL·min-1( 0-5 min,52%A→70%A; 5-7 min,70%A→80%A; 7-10 min,80% A→ 52% A; 10-12 min,52% A). The injection volume was 5 μL. The UV detection wavelength was set at 269 nm. Results: The relationship between the concentration and the peak area of cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡAwas all in good linearity( r 0. 999) over the linear ranges. The average recoveries( n = 6) of the investigated compounds were 96. 7%( RSD = 1. 3%),96. 4%( RSD = 0. 94%) and 98. 5%( RSD = 1. 2%); the chromatographic peaks of investigated compounds and coexisting components were well isolated. Conclusion: Compared with routine liquid chromatography,this method with high separation efficiency and good repeatability can be used as a reference for improvement of laboratory methods and make preparation for improvement of the quality standard of tanshinone extract.
【Fund】: 国家“重大新药创制”科技重大专项“药物安全评价技术平台”(2012ZX09302001)
【CateGory Index】: R284.1;O657.72
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 SONG Min HANG Tai-jun~* ZHANG Zheng-xing (Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China);Studies on the fingerprints of the liposoluble components of Salvia miltiorrhiza Bge.by HPLC using fingerprint reference material[J];Chinese Journal of Pharmaceutical Analysis;2007-01
2 WU Wei-bao,TANG Jun (Liu'an Institute for Drug Control,Liu'an 237006,China);UPLC simultaneous determination of 5 lipophilic components in Salvia miltiorrhiza Bge[J];Chinese Journal of Pharmaceutical Analysis;2009-05
3 ZHU Yan-qin1,YIN Qin-hong2,WANG Wen-hui1,YANG Jun1,YANG Chao-fen1(1.Research Center for Analysis and Measurement,Kunming University of Science and Technology,Kunming 650093,China;2.Kunming Jida Pharmaceutical Co.,Ltd,Kunming 650106,China);Determination of six components in Salvia yunnanensis Roots by HPLC[J];Chinese Traditional Patent Medicine;2011-04
4 ZHANG Liang,PENG Peng,ZHANG Xin-feng,LIU Song-chan,HE Min,JIANG Jian,QIU Fu-rong(Lab of Pharmacokinetics,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China);Determination of cryptotanshinone,tanshinoneⅠ and tanshinoneⅡ_A of 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations by HPLC[J];Chinese Traditional Patent Medicine;2012-06
5 JIN Zhang-zhao, ZHU Ming, ZHANG Wen-ting, QI Yan-fei (Zhejiang Institute for Drug Control, Hangzhou 310004, China);Studies on relationship between fingerprints of hydrophilic and hydropholic components in Salvia miltiorrhiza[J];Chinese Traditional and Herbal Drugs;2004-10
6 CHEN Lei1,LU Yin1,2,ZHENG Shi-zhong1,2(1.Institute of Traditional Chinese Medicine,Nanjing University of Traditional Chinese Medicine,Nanjing210029,China;2.Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China);Integration effect on pharmacological component in Salvia miltiorrhiza[J];Chinese Traditional and Herbal Drugs;2009-03
7 LIU Gang 1, ZHANG Qi-ming 2, ZHANG Jie 1, GAO Wen-juan 1, CHANG Hua 1, TAN Sheng-jian 1 (1. Department of Pharmacy, Hospital 306 PLA, Beijing 100101, China; 2. National Instiute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China);Determination of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ_A in tanshinone ear drops by HPLC[J];Chinese Pharmaceutical Journal;2004-03
8 LU Feng~(1),LE Jian~(2),CHEN Gui-liang~(2),WU Yu-tian~(1)(1.School of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.Shanghai Institute for Drug Control,Shanghai 200233,China);Analysis of and countermeasures against traditional Chinese medicines adulterated by synthetic drugs[J];China Journal of Chinese Materia Medica;2007-01
9 LIU Jing,DAI Zhong,WANG Gang-li,LIN Rui-chao*(National Institutes for Food and Drug Control,Beijing 100050,China);Progress in Bioactive Constituents and Isolation and Analysis Methods of Salvia Miltiorrhizae Radix et Rhizoma[J];Chinese Journal of Experimental Traditional Medical Formulae;2012-11
10 Liu Mingli and Ma Shuangcheng(National Institutes for Food and Drug Control,Beijing 100050);Discussion on the Rapid Screening Test and Works Need to Be Strengthened during Application Management[J];Chinese Pharmaceutical Affairs;2012-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yuan et al(Biochemical Engineering College,Beijing Union University,Beijing 100023);Effect of Chitooligosaccharides Plant Growth Regulators on the Growth and Secondary Metabolites of Salvia mitiorrhiza Bge[J];Journal of Anhui Agricultural Sciences;2010-34
2 LI Yi-long1,LI Lei1,WANG Fa-long1,YANG Lan2,SHUAI Zhao-xia3,CHEN Guo-qian1 (1Department of Medical Laboratory,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi 214023,China;2Department of Clinical Laboratory,Wuxi Maternal and Child Health Hospital Affiliated to Nanjing Medical University,Wuxi 214002,China;3Department of Clinical Laboratory,the Second Affiliated Hospital of Wannan Medical College,Wuhu 241000,China);Suppressive effect of tanshinone ⅡA on HMGB1 release from lipopolysaccharide-induced macrophages[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-09
3 LI Yin,LV Jian-Wei,FENG Xiu-fang(Dept.of Pharmacy of Traditional Chinese Hospital of LiuZhou,Liuzhou,545001;HaiNan Medical College,HaiKou,571101);Study on Compatible Stability of Danshen Injection in six Transfusions by UV Similarity[J];Journal of Chengdu University of Traditional Chinese Medicine;2009-03
4 YANG Ping, JIA Yu-hua, LI Jie, ZHOU Feng-hua, LI Li-jun Southern Medical University, Guangzhou 510515, China;Anti-oxidation of Tanshinone Ⅱ_A and Prohibitin on Cardiomyocytes[J];中草药(英文版);2010-03
5 Ke Changyi,Chen Weifeng(Department of Pharmacy,Third People's Hospital of Chongqing,Chongqing 400014,China);Retrospective analysis of 105 cases of adverse reaction of salvia miltiorrhiza injection[J];Chongqing Medicine;2011-18
6 ZHONG Guo-cheng,ZHANG Li-wen,ZHANG Li,YANG Rui-wu,DING Chun-bang(College of Biology and Science,Sichuan Agricultural University,Ya'an 625014,China);A study on photosynthetic characteristics of different Salvia miltiorrhiza varieties[J];Acta Prataculturae Sinica;2011-04
7 CHU Hui-juan,ZHANG Jin-jin,WANG Zhe,WU Yan,ZHANG Yi(College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China);Optimization and Application of MSAP Molecular Marker Reaction System of Salvia miltiorrhiza by Orthogonal Design[J];Acta Botanica Boreali-Occidentalia Sinica;2011-05
8 ZHU Yan-Qin YIN Qin-Hong a YANG Jun YANG Chao-Fen SUN Xiao-Dong(Research Center for Analysis and Measurement,Kunming University of Science and Technology,Kunming 650093,P.R.China)a(Kunming Jida Pharmaceutical Co.,Ltd,Kunming 650106,P.R.China);Research and Development of Chemical Constituents in Salvia yunnanensis C.H.Wright and Its Pharmacological Effects[J];Chinese Journal of Spectroscopy Laboratory;2012-03
9 ZHOU Dian-feng(The Physics Department of Yancheng Teachers University,Yancheng 224002,China);Infrared Spectroscopic Analysis of Some Injections from Chinese Traditional Medicine[J];The Journal of Light Scattering;2009-01
10 WENG Xin-xin1,LU Feng1,WANG Chuan-xian2,QI Yun-peng1 1.Second Military Medical University,Shanghai 200433,China2.Shanghai Exit-Entry Inspection and Quarantine Bureau,Shanghai 200135,China;Discriminating and Quantifying Potential Adulteration in Virgin Olive Oil by Near Infrared Spectroscopy with BP-ANN and PLS[J];Spectroscopy and Spectral Analysis;2009-12
China Proceedings of conference Full-text Database 3 Hits
1 YE Longhui CHENG Jun ZHOU Li HU Xiaorong (Chengdu University of Technology, College of material and chemistry & chemical engineering,Chengdu,610059);Study on the content distribution of Fe,Al,Zn,Cu and Mn and its speciation in Salvia Miltiorrhiza[A];[C];2009
2 CHEN Wen1,GUO Li-li1,ZHOU Ting-ting 2,WANG Jie1 Cardiovascular Disease-Syndrome Pattern Research Laboratory,Guang’anmen Hospital,China Academy of Traditional Chinese Medicine,Beijing 100053,China Life science and Biopharmaceutical College,Shenyang Pharmaceutical University,Shengyang 110016,China;The protective effect of total salvianolic acid on myocardium injury induced by ischemia-reperfusion on isolated rat hearts[A];[C];2010
3 Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091;Effect of Qidan Liquid on Myocardial Ischemic Injury in Rats with Acute Myocardial Infarction[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Yuan Shu-Lan, Wang Xiu-Jie, Wei Yu-Quan Key Laboratory of Biotherapy of Human Diseases,Ministry of Education,West China Hospital, Sichuan University, Chengdu,Sichuan,610041, P.R.China;Anticancer Effect of Tanshinone and Its Mechanisms[J];Chinese Journal of Cancer;2003-12
2 ZHU Xiong Xiang, TANG Chao Wu, CHEN Bi Department of Burn Surgery, Center of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;Effect of burn patient's serum and Salvia miltiorrhiza on chemiluminescence magnitude of normal blood polymorphonuclear neutrophils[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-10
3 HUANG Xi-ru~*,CAO Dong and ZHAN Wen-hong(Department of analytical chemistry,The School of pharmaceutical sciences,Hebei Medical University,Shijiazhuang 050017);Determination of cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA in compound danshen tablet by RP-HPLC[J];Chinese Journal of Analysis Laboratory;2006-08
4 BI Xian,LI Tong-Hua *,WU Liang [WT5”BX](Department of Chemistry,Tongji University,Shanghai 200092,China) [WT5HZ];Application of Independent Component Analysis to the IR spectra Analysis[J];Chemical Research In Chinese Universities;2004-06
5 YANG Zai-jun2, 3, ZHANG Li2, 3, YANG Rui-wu1, 2, DING Chun-bang1, 2, ZHOU Yong-hong2, WAN De-guang31. College of Biology and Science, Sichuan Agricultural University, Yaan 625014, China2. Key Laboratory of Crop Genetic Resources and Improvement of Ministry of Education, Sichuan Agricultural University, Yaan625014, China3. Pharmacy School of Chengdu University of Traditional Chinese Medicine, Chengdu 610015, China;Principal Component and Cluster Analysis of Trace Elements in Chinese Herb Salvia Miltiorrhiza and Its Relative Species[J];Spectroscopy and Spectral Analysis;2008-10
6 Wang Chaolin(Department of Pathophysiology,Guangxi Medical University,Nanning\ 530021)Arsalan Kharazmi,Viggo Faber(Department of Clinical Microbiology,Rigshospitalet,Copenhagen DENMARK);MODULATION OF HUMAN NEUTROPHIL AND MONO CYTE CHEMOTAXIS AND SUPEROXIDE RESPONSES BY SALVIA MILTIO RRHIZA BUNGE(SM)[J];ACTA ACADEMIAE MEDICINAE GUANGXI;1997-03
7 FANG CHI-NIEN;CHANG PEI-LING;HSU TSONG-PEI Institute of Materia Medica,Chinese Academy of Medical Sciences;THE ANTIBACTERIAL COMPONENTS OF DAN-SHEN[J];Acta Chimica Sinica;1976-03
8 SONG Zhan Jun,DONG Fang Ting,WEI Kai Hua,LIANG Yue Qin,WU Sheng Ming,ZHAO Fa,YANG Gen Suo,LIU Bin Yu,ZHENG Yong Hong,YANG Song Cheng (National Center of Biomedical Analysis,Beijing 100850 China);Identification of sildenafil in the patent Chinese medicine-Traditional Chinese Viagra by FTIR[J];Pharmaceutical Journal of Chinese People's Liberation Army;2003-05
9 Xu Qiang,Ni Shen,Feng Ying (Zhenjiang Research Institute of Chinese Meteria Madica,Zhengjiang 310023);Determination of TanshinoneⅡA of Fujin Granules by TLC-scanning[J];PRIMARY JOURNAL OF CHINESE MATERIA MEDICA;2000-03
10 WANG Yan, LING Li-mei(Huangshan Institute for Drug Control,Huangshan 245000, China);Determination of Cryptotanshinone in Danshentong Capsules by HPLC[J];Research and Practice on Chinese Medicines;2009-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved