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《Chinese Journal of Pharmaceutical Analysis》 2015-03
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Determination of five effective components in Yushu tablets by UPLC

XIA Zhong-ting;BAO Li-ying;HUI Ting-ting;DENG Yan-ru;ZHOU Shui-ping;Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis,Tianjin University of Traditional Chinese Medicine(TCM);Division of Modernized TCM,Taslyholded Group Co.,Ltd.;School of Integrative Medicine,Tianjin University of TCM;  
Objective: To establish a UPLC method for the simultaneous determination of five effective components( albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin) in Yushu tablets. Methods: The analysis was performed on a Waters Acquity UPLC system with a Waters ACQUITY UPLCTMBEH C18column( 100 mm × 2. 1 mm,1. 7μm). The five effective components were separated with a gradient mobile phase consisting of 0. 1% phosphoric acid aqueous( A)- acetonitrile( B) at a flow rate of 0. 4 m L·min- 1,and the UV detection wavelength was set at203 nm,the column temperature was 30 ℃. Results: The contents of albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin in the corresponding linear ranges showed good linear relationships( r≥0. 999 0); the average recoveries( n = 6) of the five components were 101. 4%( RSD = 0. 82%),101. 9%( RSD = 1. 0%),99. 0%( RSD =2. 6%),98. 9%( RSD = 2. 0%),100. 0%( RSD = 2. 4%),respectively. In the 3 batches of samples,the contents of albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin were in the ranges of 3. 53- 3. 80,9. 33- 10. 01,0. 89-0. 98,0. 72- 0. 76,and 0. 82- 0. 86 mg per tablet. Conclusion: The developed method is time- saving and easy to operate,which can be used as a method of quality control of Yushu tablets.
【Fund】: 国家“重大新药创制”科技重大专项(2013ZX09402202)
【CateGory Index】: R286.0;O657.72
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