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《Chinese Journal of Pharmaceutical Analysis》 2015-09
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HPLC fingerprint of Lamiophlomis rotata

ZHONG Shi-hong;GU Rui;LIAO Yan-feng;WANG Ling-xin;ZHENG Huan;ZHENG Xiao-hua;HUANG Ming-gui;Pharmaceutical School,Chengdu Medical College;Chengdu University of TCM;  
Objective: To establish a fingerprint analysis method for quality evaluation of Lamiophlomis rotata.Methods: HPLC was employed,and the separation was performed on a Welchrom- C18 chromatographic column( 250 mm × 4. 6 mm,5 μm) using acetonitrile( A)- 0. 1 % orthophosphoric acid( B) as mobile phase with gradient elution( 0-10 min,5 % A→8 % A; 10-27 min,8 % A→12 % A; 27-35 min,12 % A; 35-70 min,12 % A→17 % A; 70-80 min,17 % A →19 % A; 80-110 min,19 % A →36 % A; 110-120 min,36 % A →50 %A) at a flow rate of 1. 0 m L·min- 1. The detection wavelength was set at 235 nm and the column temperature was controlled at 30 ℃. The data were analyzed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2004A). Results: The total 23 batches of aerial part of Lamiophlomis rotata were evaluated,and fourteen common chromatographic peaks were observed. Then,the No. 3,No. 4,No. 5,No. 8,No. 9,No.10,No. 11 and No. 12 peaks were identified as sesamoside,shanzhiside methylester,chlorogenic acid,loganin,8-Oacetyl shanzhiside methylester,forsythiaside B,luteolin glycosides and acteoside,respectively. Conclusion: The established method is more comprehensive and accurate in evaluating the quality of Lamiophlomis Herba and can provide the scientific basis for quality control.
【Fund】: 国家自然科学基金资助项目(编号:81403040)
【CateGory Index】: R284.1;O657.72
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