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《Chinese Journal of Pharmaceutical Analysis》 2015-10
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Simultaneous determination of the content of seven components in Kanggan granules by HPLC wavelength switching technology

GUO Dong-xiao;LIN Lin;WANG Bing;CUI Wei-liang;LIU Hong-chao;XU Li-hua;LIN Yong-qiang;Shandong Institute for Food and Drug Control;  
Objective: To develop an HPLC method for simultaneous determination of seven components in Kanggan granules. Methods: The determination was carried out with Kromasil 100- 5C18column( 250 mm × 4. 6 mm,5μm). The mobile phase consisted of acetonitrile( A)-0. 4% phosphoric acid solution( B) with gradient elution( 0-14 min,8% A "16% A; 14-22 min,16% A; 22-23 min,16% A "24% A; 23-38 min,24% A; 38 – 39 min,24% A "8% A) at a flow rate of 1. 0 m L·min#1at 10 ℃. The detection wavelengths were set at 327 nm in 0-22 min to determine 3-O-caffeoyl quinic acid,chlorogenic acid( 5-O-caffeoyl quinic acid),and 4-O-caffeoyl quinic acid; 230 nm in 22-26 min to determine paeoniflorin; and 327 nm in 26-38 min to determine 4,5-O-dicaffeoyl quinic acid,3,5-O-dicaffeoyl quinic acid,and 3,4-O-dicaffeoyl quinic acid. Results: The linear ranges of 3-O-caffeoyl quinic acid,5-O-caffeoyl quinic acid,4-O-caffeoyl quinic acid,paeoniflorin,4,5-O-dicaffeoyl quinic acid,3,5-O-dicaffeoyl quinic acid,and 3,4-O-dicaffeoyl quinic acid fell within the ranges of 0. 010 7-0. 214 6μg,0. 044 9-0. 898 0 μg,0. 010 0-0. 199 6 μg,0. 099 9-1. 998 6 μg,0. 010 3-0. 206 2 μg,0. 010 6-0. 211 4 μg,and 0. 010 1-0. 201 4 μg,respectively. The average recoveries( n = 6) were 101. 6%,102. 0%,101. 1%,101. 6%,102. 0%,100. 6%,and 102. 1%; the relative standard deviations were 0. 9%,0. 9%,0. 6%,0. 8%,1. 5%,1. 0%,and 0. 5%,respectively. The content ranges of seven aforementioned components in ten samples were 0. 040-0. 640 mg·g#1,0. 157-1. 639 mg·g#1,0. 061-0. 850 mg·g#1,6. 519-9. 225 mg·g#1,0. 010-0. 339 mg·g#1,0. 021-0. 353 mg·g#1,0. 046-0. 635 mg·g#1,respectively. Conclusion: The established method for the determination of multi-components is accurate,reliable,and reproducible,which can be applied to the quality control of Kanggan granules.
【Fund】: 国家重大新药创制中药质量安全检测和风险控制技术平台(2014ZX09304307-002);; 山东省自然科学基金项目(ZR2013HM074)
【CateGory Index】: R286.0;O657.72
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